Cloning and Sequencing of a cDNA Encoding the Coat Protein of an Egyptian Isolate of Pepper Mild Mottle Virus
Cloning and Sequencing of a cDNA Encoding the Coat Protein of an Egyptian Isolate of Pepper Mild Mottle Virus
S.A. Sidaros, S.A. El-Kewey , Hala A. Amin;Eman A.H. Khatab , A.A. Emeran l , Samaa Abd El-Khalilk and M.A.S. El-Kady
ABSTRACT
A Tobamovirus isolated from pepper plants grown in Egypt has been characterized. Pepper mild mottle Tobamovirus (PMMoV) was isolated from naturally infected pepper plants grown in Kafr El-sheikh Governorate. RT-PCR using a specific non:åegenerate primer pair for the PMMoV coat protein gene (PMM-F and PMM-R) revealed 470 bp amplified product. Dot blot hybridization was used to establish the authenticity and specificity to the RT-PCR amplified Products of PMMoV. The coat protein gene of an Egyptian isolate of PMMoV was cloned and sequenced, The sequence contained a full-length ORF coding for the viral CP. It comprises 473 nt and a polypeptide chain of 157 amino acids with a M(r) of 17.27. The comparison of the CP ORF nucleotide sequence of Egyptian isolate (PMMoV-E2) with those of another isolates revealed 'that it was very close to the Brazilian and Japanese isolates (99 % and 98% respectively). Whereas the cp of PMM0V-E2 isolate was closely related to TMV-CP (99 & 97%) of Japan and Korea isolates, respectively. In the meantime it appeared far from Tropica Soda Apple Mosaic Virus-CP (TSAMV-CP) Florida isolate (USA) of Tobomovirus
To share on other social networks, click on any share button. What are these?
