Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato Plants
Evaluation of Some Therapies to Eliminate Potato Y Potyvirus from Potato Plants
Sabry Y. M. Mahmoud; Maher H. Hosseny and Mamdouh H. Abdel-Ghaffar
ABSTRACT
Viral infection of potato (Solanum tuberosum L.) plants is very important due to their effect on potato yield and degeneration of seed tubers. This study aimed to eliminate potato virus Y (PVY) by tissue culture technique using different therapies, such as thermo-, chemo- and electrotherapies. Potato plants cv. Diamond cultivated at Faculty of Agriculture farm, Sohag University were tested by direct antigen coatingenzyme linked immunosorbent assay (DAC-ELISA) using antisera against PVY, Potato virus X (PVX) and Potato leaf roll virus (PLRV). The results indicated the occurrence of single and mixed infections of three viruses in potato plants. Survey results indicated highly distribution of PVY infected plants, which its yield was affected strongly. Tubers of potato plants which gave a positive reaction to PVY only were collected at harvest, stored in cold store room for three months and then planted in a greenhouse to obtain materials for virus elimination studies. Virus status in vitro plantlets was determined by double antibody enzyme linked immunosorbent assay (DAS-ELISA) and biologically test before and after treatments The two cycles of thermotherapy at approximately 370C, during 40 and 30 days, resulted in 33.3 and 14.2% PVY elimination, respectively. Thus, these results indicated that thermotherapy is moderately efficient for viral eradication. Chemotherapy was undertaken with 20 mg Ribavirin (RBV); it is showed the medium rate of virus elimination (30.0 - 42.9% virus-free plantlets) when added to the tissue culture media. Several stems including axillary buds were excised from the potato plants grown for 45 days and electric-shocked treated. Stems were directly connected to the electrodes of power supply or indirectly by immersion of plant tissue in electrified water in a wide range of intensity-time. Axillary buds excised from electric-shocked stems or tissues immersed in electrified water, were transferred into the medium supplemented with or without ribavirin (RBV) •to examine its combination effect. With an electric shock treatments alone (5, 10 and 15 mA electric current), the replicates become PVY-free in rates53.8,72.7 and 87.5%, respectively. Simultaneous, RBV and electric shock had higher efficiency for PVY elimination, reaching rates of healthy plantlets of 66.7% with 5 mA, 90.0% with 10 mA, and 100.0% with 15 mA.
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