Curcuma Longa Methanolic Extract and Losartan Improves Memory Impairment and Oxidative Stress induced by a High Caloric Diet in Wistar Rats
Curcuma Longa Methanolic Extract and Losartan Improves Memory Impairment and Oxidative Stress induced by a High Caloric Diet in Wistar Rats
Sara Brikat, Mouloud Lamtai*, Miloud Chakit, Laila Ibouzine-Dine, Ilhame Fitah, Oumaima Abouyaala, Abdelhalem Mesfioui, Aboubaker El Hessni
ABSTRACT
The objective of the current study was to assess the methanolic extract of Curcuma longa (Curc) and losartan on memory impairment induced by a high fructose diet (HFD) in Wistar rats. 36 female Wistar rats aged 2 months and weighing 74 to 100 g were randomly divided into 6 groups as follows: 2 groups would receive a standard diet which a group serve as control and a group receive 100 mg/kg/d of Curc and 30 mg/kg/d of Losartan (Curc + Los), 4 groups would receive the high HFD diet: one group serve as control (HFD), a group will receive 100 mg/kg/day of Curc, qualified (HFD + Curc), a group will receive 30 mg/kg/day of Losartan, qualified (HFD + Los) and a group will receive HFD + Curc + Los. After 8 weeks of experience, working and recognition memory were evaluated using the Y-maze and Object Recognition (OR) tests. The oxidative stress (OS) was assessed by measuring the levels of Thiobarbituric acid reactive substances (TBARS), nitric oxide (NO) and catalase activity (CAT) in the hippocampus. The administration of Curc and/or Los increased significantly the recognition index (p < 0.01) as compared with the HFD group, and were able to prevent this effect on the memory induced by the HFD (p < 0.01). Additionally, the levels of TBARS and NO in HFD group significantly exceeded the respective values in female animals when compared to control rats (p < 0.05). In contrast, the administration of HFD + Curc + Los lowered NO levels significantly in rats in comparison with the standard diet-treated rats (p < 0.01). In summary, our results suggest that treatments with Curc and/or Los could serve as possible therapeutic agents for memory impairment and OS provoked by HFD.
Keywords | Curcuma longa, Losartan, Memory, High caloric diet, Wistar rats
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April 2024
Vol. 12, Iss. 4, pp. 586-801
