Effects of Quorum Sensing AHL Signaling on the Biological Characteristics of Porcine Derived F4ac+ Enterotoxigenic Escherichia coli
Yang Yang1,2, Ji Shao1,2, Mingxu Zhou3,4, Qiangde Duan1,2, Xinyi Zhang1,2 and Guoqiang Zhu1,2*
1Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China
2College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China
3Institute of Veterinary Immunology and Engineering, National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
4Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China
Yang YangandJi Shao contributed equally to this work.
* Corresponding author: yangf@aset.ac.cn
Fig. 1.
The signal molecule detection of AHL. A, Detection the ability of C83902/pyenI to synthesize short side chain AHL by reporter strain CV026. B, Detection the ability of C83902/plasI to synthesize long side chain AHL by reporter strain JZA1. C6-HSL and 3OC12-HSL were used as positive controls.
Fig. 2.
The growth curves of C83902 and its recombinant strains under endogenous (A), and exogenous AHLs influence (B).
Fig. 3.
Quantitative test of biofilm formation of C83902 and its recombinant strains under endogenous (A) and exogenous AHLs influence (B).
Fig. 4.
The adherence ability of IPEC-J2 cells of C83902 and its recombinant strain under endogenous (A), and exogenous addition of AHL (B). **, P<0.01; ***, P<0.001.
Fig. 5.
Transcriptional levels of virulence related genes of C83902 and its recombinant strains endogenous and exogenous C6-HSL (A) and OC12-HSL (B). * P<0.05