Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation
Enhanced Bacterial α-Amylase Production Using Mutant Strains Through Submerged Fermentation
Muhammad Adeel Farooq1, Shaukat Ali1*, Ali Hassan1, Rida Sulayman1, Muhammad Ahsan Kaleem2, Hafsa Shahzad1, Muhammad Summer1, Arooj Latif1, Tahreem Tanveer1
Production of α-amylase (U/ml) obtained by wild and different mutant strains of (A) Bacillus subtilis and (B) Bacillus licheniformis is compared, using medium M1 containing Soya bean meal as agro-waste. Each bar represents SEM (standard error of the mean) for the mean of three replicates. Different alphabets on different columns represent that these are statistically different (p< 0.001).
Biosynthesis of α-amylase (U/ml) obtained using different media containing different agro-wastes like soybean meal M1, wheat bran M2, apple peels M3, rice hull M4, cucumber peels M5 by (A) BSAA-25 and (B) BLAA-25 strains. Each bar represents SEM for the mean of three replicates. Different alphabets on different columns represent that these are statistically different (p< 0.001).
Influence of different temperatures and pH values on the biosynthesis of α-amylase produced by BSAA-25 and BLAA-25. Influence of temperatures on the biosynthesis of α-amylase by (A) BSAA-25 and (B) BLAA-25 strains. Influence of pH values on the biosynthesis of α-amylase produced by (C) BSAA-25 and (D) BLAA-25 strains, using the medium M2 containing agro-waste (wheat bran). Each bar represents SEM for the mean of three replicates (p< 0.001).
Influence of different temperatures, pH values and concentrations of starch solution on the activity of α-amylase (U/ml) synthesized by BSAA-25 and BLAA-25. Influence of different temperatures on the activity of α-amylase (U/ml) synthesized by (A) BSAA-25 and (B) BLAA-25 strains. Influence of pH values on the activity of α-amylase (U/ml) synthesized by (C) BSAA-25 and (D) BLAA-25 strains. Influence of the concentrations of starch solution on the activity of α-amylase (U/ml) synthesized by (E) BSAA-25 and (F) BLAA-25 strains, utilizing the medium M2 containing agro-waste (wheat bran). Each bar represents SEM for the mean of three replicates (p< 0.001).
