Evaluating the Efficacy of Berberine Supplement versus Ozempic® Injection in Modulating Glycemic State, Lipid Metabolism and Body Weight in Streptozotocin-Induced Diabetic Rats
Evaluating the Efficacy of Berberine Supplement versus Ozempic® Injection in Modulating Glycemic State, Lipid Metabolism and Body Weight in Streptozotocin-Induced Diabetic Rats
Wassan Mhammed Husain1*, Israa J. Mohammad2, Dahlia N Al-Saidi1
ABSTRACT
The goal of this study was to see the effect of berberine Hcl supplement versus the effect of Ozempic® injection on body weight, glycemic states, and lipid profile in diabetic rats. The experiment includes two parts: in the first part, forty adult rats with a body weight of 250 ± 50 are randomly divided into two groups. G1: are a control group, twenty rats intraperitoneal (IP) injection of normal saline twice weekly along with a normal (pellet) diet for four weeks. G2: are twenty rats induced diabetes by intraperitoneal injection of a low dose of Streptozotocin (35 mg/kg. BW) twice weekly along with a high-cholesterol diet for four weeks. Rats with fasting blood glucose greater than 190 mg/dl were considered diabetic animals. The second parts include three groups T1: Six diabetic rats were orally administered distilled water and subcutaneously injected with 0.25 mg/kg BW of normal saline for four weeks. T2: Six diabetic rats, orally administered with berberine Hcl 100 mg/kg B.W. and subcutaneously injected with 0.25 mg/kg B.W. normal saline one time per week for four weeks. T3: six diabetic rats treated by subcutaneously injecting Ozempic® 0.25 mg/kg B.W. one dose per week for four weeks with orally administration of distilled water. The result of first part were showed significant (p≤0.00001) elevation in the body weight from (250±50 gm) of the control group to the (420±30 gm) in the STZ group also elevated in the fasting blood glucose from (90mg/dl) in the control group to the (270 mg/dl) in STZ group. Result of second part of experiment to the body weight and blood collection at three times (zero time, 2nd week, and 4th week) for fasting blood glucose, insulin, insulin C-peptide, and lipid profile measurement were display a significant (p≤0.05), (p≤0.00001) decrease in body weight, fasting blood glucose, insulin, insulin C-peptide, and lipid profile in diabetic rats after treatment with berberine supplement and Ozempic® injection. When compared with zero time in the T1 group. At the end of the experiment, we can suggest the ability of 100 mg/kg B.W. of berberine supplement and 0.25 mg/kg B.W. of Ozempic® injection to control unintentional weight gain and improve glycemic status and lipid profile in STZ-induced diabetes within short treatment times (four weeks) and we recommend the further experiments with longer time (three month) for more current result.
Keywords | Ozempic® injection, Semaglutid, Berberine Hcl, Streptozotocin, Type two diabetes, Lipid profile, Insulin resistance
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