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Evaluation of the Anti-Coccidial Effect of Cashew Oil and/or Toltrazuril in Chickens Experimentally Infected with Eimeria tenella

PJZ_56_6_2859-2868

Evaluation of the Anti-Coccidial Effect of Cashew Oil and/or Toltrazuril in Chickens Experimentally Infected with Eimeria tenella

Kamal Ahmed El-Shazly1, Amira Abd El-Latif1, Nagwa Elhawary2, Asmaa F. Alswaf1, Foad Farrag3, Samy Sayed4, Mohamed El-Sharnouby5 and Mustafa Shukry6*

1Pharmacology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

2Parasitology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

3Anatomy and embryology department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

4Department of Science and Technology, University College-Ranyah, Taif University, B.O. Box 11099, Taif 21944, Saudi Arabia

5Department of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia

6Physiology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt

ABSTRACT

This research defined assessed the therapeutic effect of cashew oil, toltrazuril, and their combination to broiler chickens experimentally induced cecal coccidiosis. For this study, divided randomly one hundred broiler chicks at 15th-day old into equal five groups (20 each). kept G1 as control negative; G2, non-treated infected; G3, infected treated with cashew oil (1.5ml/liter drinking water); G4, infected treated with toltrazuril (2.5ml/liter drinking water); G5, infected treated with a mixture of cashew oil at a dose of 1ml and toltrazuril 1.25ml/liter drinking water. On the 15th the oocysts of the Eimeria tenella parasite were implanted orally into all but the negative control groups. According to the current findings, G2 demonstrated a significant increase in feed conversion rate, oocysts count, lesion score, mortality rate, count total leucocytic, ALP, ALT, AST, total bilirubin, creatinine, urea, uric acid, and MDA level, the body weight significally reduction, weight gain in the body, feed consumption, hematological parameters, lymphocytes%, cholesterol level, and Catalase enzyme activity. These results followed the histopathological findings. Treatment with the tested drugs improved all the tested parameters. Still, the control of coccidiosis caused by Eimeria tenell is highly effective by combination of cashew oil and toltrazuril.


Article Information

Received 04 August 2022

Revised 06 August 2022

Accepted 10 August 2022

Available online 02 June 2023

(early access)

Published 31 October 2024

Authors’ Contribution

KAE, AAE and NE designed, performed, and supervised experiments, critically revised and finalized the manuscript. AFA, FF, SS, ME and MS performed experiments, wrote and finalized the manuscript. ME and MS analyzed data. AFA, FF and SS interpreted data. All authors have read and approved the final version of the manuscript.

Key words

Broilers, Coccidiosis, E. tenella, Experimental infection, Toltrazuril, Cashew oil

DOI: https://dx.doi.org/10.17582/journal.pjz/20220804120813

* Corresponding author: [email protected]

0030-9923/2024/0006-2859 $ 9.00/00

Copyright 2024 by the authors. Licensee Zoological Society of Pakistan.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).



INTRODUCTION

The administration of anti-coccidial drugs is a highly effective method of controlling coccidiosis (Soutter et al., 2020). The anti-coccidial drugs control coccidiosis by inhibiting Eimeria sp.’s proliferation (Craig et al., 2020). Toltrazuril is a highly effective anti-coccidial drug. It is effective against all Eimeria infecting chicken and has activity against all of the Eimeria sp.’s developing intracellular phases (Mathis et al., 2003).

The search for products derived from natural sources and plants has increased in the last years to produce drug-free birds. These natural origin compounds related to their ability to improve immune response, body weight gain, and destroy Eimeria oocysts. Also, the development of new drugs to avoid the main problem of drug resistance to control avian coccidiosis and make poultry valid for human consumption (Quiroz-Castañeda, 2018). Among the natural products is the cashew nut shell oil (Anacardium occidentale L.), which comprises anacardic acid, cardanol, cardol, and 2-methylcardol acids (Trevisan et al., 2006). These phenolic oil components biological activities have attracted considerable attention due to their activity as molluscicidal (Kubo et al., 1986), activity as anti-tumor (Itokawa et al., 1989), activity as antimicrobial (Kubo et al., 2003), and properties as antioxidant (Abreu et al., 2017). The synergistic antimicrobial property to cashew nut shell oil with other antibiotics is fascinating (Muroi and Kubo, 1996). The commercial mixture of castor oil and cashew nut liquid reduced the excretion of oocysts and increased the heterophile/lymphocyte ratio (Collares et al., 2018). The study of Toyomizu et al. (2003) during experimental coccidial infection provides the first evidence of the reducing effects to the severity of cecal lesions in chickens by cashew nut shell oil.

The aim of this study was estimation the effects of cashew oil compared to toltrazuril, administered alone or in combination at their recommended oral doses against cecal coccidiosis in broiler chickens. Moreover, the growth performance, hematological, biochemical, histopathological, and antioxidant effects in chicken were also examined.

MATERIALS AND METHODS

Chemicals

The diagnostic kits were obtained from Bio-Diagnostic Co., Giza, Egypt to assaying kidney and liver function tests, the lipid peroxidation levels, and antioxidants. Toltrazuril (Toltrasol 2.5 % oral solution) ® was obtained from ARABCOMED, Egypt. Cashew oil (Cardox 200) ® was obtained from Souter Trade Company for importing and trading veterinary pharmaceuticals, Egypt.

Collection and preparation of Eimeria tenella oocysts

The isolated oocysts from the ceci of chickens naturally infected. They were separated using sieving and sedimentation techniques (Soulsby, 1968). The sporulated Eimeria oocysts were kept in 2.5% potassium dichromate solution at 4oC until their use for experimental infection.

Experimental design and grouping

Using one hundred chicks broiler chicks of the Cobb strain, one-day old. The program of vaccination was conducted using the Hitchner BI strain (eye drops on the 7th day of age). At the 13th day of age, the Gambero vaccine and ND clone 30 were administered in drinking water. The birds fed on anti-coccidial drug free and balanced ration. Five groups of birds were formed. Five groups of birds were formed (20 chicks each). G1 (control negative), G2 (control positive), G3, infected + cashew oil at a 1.5ml/liter drinking water (according to Souter Trade company), G4, infected + toltrazuril at 2.5ml/liter drinking water (according to ARABCOMED company, Egypt) and G5: Infected + a mixture of cashew oil at a dose of 1ml and toltrazuril at 1.25ml/liter of drinking water. All except one of the groups (G1) were exposed to oocysts E. tenella. Infection was performed orally on the 15th day of age by a single dose of 5×104 sporulated oocysts. Administered the drugsfrom the 5th day post-infection for three successive days in the drinking water. All groups were kept under observation until the end experiment (36th day of age).

Criteria of the efficacy of the tested drugs

Growth performance parameters

Chickens of each group were individually marked and weighed before feeding at the beginning of the experiment, on 15th day of age the day of infection, and then weekly till the end of the investigation. The body weight (BW), body weight gains (BWG), feed intake (FI) and feed conversion ratio (FCR) were observed according to Davis et al. (1986) and Seddiek et al. (2008).

Oocysts reducing percentage

Daily collected three fresh fecal samples spread on the ground from each group from different parts of the litter for oocysts count at day 5th until day 20th post-infection. Stored the samples in 2.5 % potassium dichromate until counting by McMaster technique (Levine, 1988).

Hematological and biochemical analysis

Blood samples were collected on the 9th and 23rd days post-infection from vein of the wing randomly selected five birds from each group. The hematological analysis following Schalm et al. (1975). The serum enzymatic activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were assessed following Reitman and Frankel (1957), serum ALP activity according to Belfield and Goldberg (1971), serum cholesterol level according to Richmond (1973), serum bilirubin level according to Walter and Gerade (1970), serum uric acid level following Barham and Trinder (1972), serum creatinine level following Larsen (1972) and serum urea level following Fawcett and Soctt (1960). the colorimetrical estimations of hepatic malondialdehyde (MDA), the primary end of lipid peroxidation product, according to the protocol of Ohkawa et al. (1979) and catalase activity following Aebi (1984).

Histopathological examination

The kidney, cecum, and part of the liver for histopathological examination were put in 10% neutral buffered formalin and examined according to Lillie (1954).

Statistical analysis

Using graph pad prism software, one-way ANOVA and Tukey multiple comparisons were used to evaluate the data. For this study, P < 0.05 considered an acceptable threshold of statistical significance.

Results

Growth performance

In the infected non-treated group (G2), body weight, weight gain, and feed consumption decreased while feed conversion ratio increased (G1). G3, G4, and G5 showed significant growth improvement over G2, with G5 showing the best outcomes (Tables I, II).

 

Table I. The effect of cashew oil, toltrazuril, and their combination on the body weight in broiler chickens experimentally infected with Eimeria tenella.

Group

Day (age)

15th

22nd

29th

36th

G1

260.1± 7.71a

588.25± 11.20a

1097.66± 20.37a

1604.00± 19.20a

G2

267.30± 7.22a

430.71± 11.23d

800.00± 10.22d

1220.88± 22.23d

G3

253.00± 9.14a

500.00± 15.38 bc

920.66± 17.41 bc

1362.27± 25.19 c

G4

253.00± 6.75a

547.61± 13.34b

1004.00± 23.42b

1450.82± 22.91b

G5

265.00± 5.23a

589.12± 10.26a

1080.00± 18.34a

1580.00± 30.51a

 

Means within rows with different letters differ at P ≤ 0.05. n = 20.

G1, negative control; G2, positive control; G3, infected + cashew oil at 1.5ml/l; G4, infected + toltrazuril at 2.5ml/l; G5, Infected + mixture of cashew oil at 1ml/l and toltrazuril at 1.25ml/l.

 

Oocysts count

The discharge oocyst in faeces developed on day 7, peaked on day 9, and dropped till day 19 post-infection. G2 produced more oocysts than the other groups. G5 had the largest reduction in faecal oocyst count, as seen in Table III.

Lesion score and mortality %

Table IV shows that G3, G4, and G5 reduced lesion score significantly compared to G2. At 9th and 23rd dpi, G5 was much lower than G2, G3, and G4. Mortality in G2 decreased 30% and in G3, G4, and G5 it decreased 15%.

Hematological parameters

G2, G3, G4, and G5 had lower RBCs, Hb, and PCV than G1. G3, G4, and G5 had significantly higher parameters than G2, with G5 showing the most improvement (Tables V, VI). There is significant improvement in the DLC in G3, G4, and G5 compared to G2.

 

Table II. The effect of cashew oil, toltrazuril, and their combination on the body weight gain (in g), feed consumption (in g) and feed conversion ratio (mean ± S.E.) in broiler chickens experimentally infected with Eimeria tenella n = 20.

Group

Day (age)

15-22

22-29

29-36

Body weight gain

G1

328.15± 2.20a

509.41± 4.27a

506.34± 3.22a

G2

163.41± 2.50d

369.29± 4.14d

420.88± 1.21c

G3

247.00± 3.11bc

420.66± 1.28bc

441.61± 2.29b

G4

294.61± 1.12b

456.39± 2.17b

446.82± 4.12b

G5

324.12± 2.20a

490.88± 5.53a

500.00± 3.21a

Feed consumption (g)

G1

687.50± 11.23a

840.61± 10.88a

1120.00± 12.58a

G2

555.35± 10.74d

760.25± 6.22cd

1090.00± 8.92c

G3

583.00± 9.23c

780.37± 11.44c

1100.00± 9.58b

G4

620.50± 5.45b

822.00± 12.25ab

1100.00± 13.34b

G5

660.00± 11.57a

845.50± 9.67a

1150.00± 16.55a

Feed conversation ratio

G1

2.09± 0.02 e

1.65± 0.01 d

2.21± 0.01 d

G2

3.39± 0.03 a

2.06± 0.02 a

2.59± 0.08 a

G3

2.36± 0.03 b

1.86± 0.01 b

2.49± 0.03 b

G4

2.11± 0.01 c

1.80± 0.06 b

2.46± 0.05 b

G5

2.04± 0.05 de

1.72± 0.08 cd

2.30± 0.08 cd

 

Means within rows with different letters differ at P ≤ 0.05. n = 20

 

Table III. The oocysts count (x 103/gm feces) (mean ± S.E.) from 7th to 19th day post-infection in broiler chickens experimentally infected with Eimeria tenella and treated with the tested anticoccidial drugs.

Day

Group

G2

G3

G4

G5

7th

24.6 ± 2.42 a

12.6 ± 2.58 bc

13.8 ± 1.66 b

11.5 ±1.22 d

8th

34.8 ± 3.16 a

10.6 ± 1.11 bc

12.2 ± 1.76 b

9.0 ± 1.66 d

9th

97.0 ± 3.58 a

9.6 ± 1.22 bc

10.4 ± 1.23 b

5.6 ± 0.45 d

10th

65.0 ± 2.68 a

9.0 ± 1.02 bc

9.8 ± 1.11 b

4.9 ± 1.18 d

11th

17.6 ± 1.12 a

8.4 ± 1.56 bc

9.0 ± 1.22 b

4.4 ± 1.23 d

12th

12.8 ± 1.76 a

7.5 ± 1.23 bc

8.1 ±1.22 b

4.0 ± 1.13 d

13th

6.8 ± 1.55 a

4.3 ± 1.02 bc

5.3 ± 1.10 b

2.9 ± 0.02 d

14th

6.1 ± 1.13 a

3.8 ± 0.88 bc

4.4 ± 1.01 b

2.6 ± 0.54 d

15th

5.8 ± 1. 08 a

2.2 ± 0.05 b

2.5 ± 0.44 b

2.0 ± 0.07 c

16th

2.8 ± 0.03 a

1.3 ± 0.03 b

1.5 ± 0.08 b

1.2 ± 0.06 c

17th

2.0 ± 0.02 a

0.0 ± 0.00 b

0.0 ± 0.00 b

0.0 ± 0.00 b

18th

1.8 ± 0.01a

0.0 ± 0.00 b

0.0 ± 0.00 b

0.0 ± 0.00 b

19th

1.5 ± 0.01 a

 

Means within rows with different letters differ at P ≤ 0.05. N= 3

 

Table IV. The effect of cashew oil, toltrazuril, and their combination on lesion score (mean ± SE) and mortality % in broiler chickens experimentally infected with Eimeria tenella.

Group

Lesion score

no. of deaths

Mortality %

At 9th dpi

At 23rd dpi

G1

00 ± 00

00 ± 00

0

00.00%

G2

3.60±0.11 a

2.60±0.09 a

6

30.00%

G3

2.60 ±0.03 b

2.20 ± 0.01 b

4

20.00%

G4

2.40 ± 0.03 b

1.80 ± 0.05 bc

4

20.00%

G5

1.60 ± 0.04 c

1.20 ± 0.05 d

3

15.00%

 

Means within rows with different letters differ at P ≤ 0.05. n= 5

 

Biochemical parameters

Tables III and IV show a considerable increase in blood ALT, AST, ALP activity, total bilirubin, urea, creatinine, and uric acid levels in G2, G3, G4 and G5 compared to G1. G3, G4, and G5 showed considerable improvements over G2. G2, G3, G4, and G5 have lower Catalase enzyme activity and higher MDA levels than G1. G3, G4, and G5 have higher catalase activity and lower MDA levels than G2.

Histopathological results

At 1st sacrifice (9th dpi)

Histopathologically, the intestines (Ceci) Covering epithelium and intestinal crypts were normal in G1 control birds. In G2, the intestinal crypts were heavily infected with coccidial stages. Cashew oil-treated chickens decreased significantly (G3). Live coccidial schizont stages decreasedin toltrazuril-treated chicken (G4). In cashew oil + toltrazuril-treated hens (G5), the mucosa was normal with dead parasite stages (Fig. 1).Normal hepatocytes are radially organised in the portal area of uninfected, untreated hens (G1). Infected, untreated hens (G2) had necrotic foci and mononuclear cell infiltration. Cashew oil (G3)-treated chickens demonstrated periportal eosinophil infiltration. Toltrazuril (G4)-treated liver demonstrated periportal eosinophilic cell infiltration. Finally cashew oil + toltrazuril hens (G5) revealed mononuclear and eosinophilic cell infiltration (Fig. 2). Non-infected, untreated chickens (G1) had normal glomeruli and tubules. Infected, untreated chickens (G2) have interstitial nephritis. Cashew oil-treated hens (G3) had interstitial nephritis. In toltrazuril-treated chickens (G4), renal tubules revealed moderate degeneration. Cashew oil + toltrazuril hens (G5) showed minor renal tubule cloudiness (Fig. 3).

 

 

 

At the 2nd sacrifice (23th dpi)

Histopathologically, non-infected, non-treated hens had normal intestines (Ceci) (G1). Untreated chickens (G2) had dead coccidial stages. In cashew oil (G3) and toltrazuril (G4)-treated hens, epithelial crypts hyperplasia and interstitial mononuclear cell infiltration occurred. In cashew oil + toltrazuril-treated chickens (G5), crypt hyperplasia was seen (Fig. 1). Hepatocytes were normal in non-infected, non-treated control chickens (G1). Non-treated infected hens (G2) had moderate hepatic vacuolation. Hepatic vacuolation was modest in cashew-oil-treated chickens (G3). Hepatocytes were normal in toltrazuril-treated (G4) and cashew oil-treated (G5) chicken livers (Fig. 2). Uninfected, untreated hens (G1) had normal renal glomeruli and tubules. E. tenella-infected untreated hens (G2) had regenerated tubular basophilia. Cashew oil-treated kidney (G3) revealed modest degenerative tubular epithelial alterations. Kidneys treated with toltrazuril (G4) and cashew oil + toltrazuril (G5) had normal glomeruli and tubules (Fig. 3).

Discussion

Anticoccidials and good management are essential for controlling and preventing coccidiosis in domestic chickens (Abdisa et al., 2019). Anti-coccidial medicines have been the subject of contemporary study. As a result, new medications must be developed to limit the spread of coccidiosis in animals that are consumed by humans (Quiroz-Castañeda, 2018).

Infection with E. tenella is characterized by rapid replication of the parasite in a host cell with extensive damage to intestinal mucosa within 4-7 days (Olanrewaju and Agbor, 2014). The feed efficiency and body weight gain in birds treated with toltrazuril better than infected non-treated birds. Toltrazuril act against all intracellular schizonts give

increased weight gains by reducing in excretion oocyst and lesion scoring (Elkhouly et al., 2016). The cashew oil has the same effect of toltrazuril improved body weight gain and decreased the lesion score (Ferket et al., 2020) and oocysts count (Quiroz-Castañeda, 2018). Also, have antibacterial activity, preventing secondary bacterial infection after infection by coccidiosis (El-Sawah et al., 2020) and improved the immune parameters (Moraes et al., 2019; Yusuf and Aliyu-Paiko, 2020). It can reduce the intestinal microbial load, resulting the toxins associated with adverse morphology changes in intestine (Xu et al., 2003). The cashew oil-treated group showed a reduction in lesion score, fecal oocysts count, and mortality % similar to the toltrazuril-treated group, which coordinated with Collares et al. (2018). They concluded that mixture of cashew nut liquid and castor oil reduced the excretion of oocysts at 7and 14 days post-challenge. Cashew act as a natural anti-coccidial drug contains alkyl phenolic oil and anacardic acid, which showed in previous studies an improvement in energy utilization, livability, and reduced the severity of the lesions in infected chickens (Farias et al., 2017). Therefore, their potential protonophore/ionophore properties, anacardic acids have the potential to be used as anti-coccidial and/or anti-inflammatory drugs (Toyomizu et al., 2003). The oil’s antioxidant effect on the host cell (Murakami et al., 2014). The E. tenella reduced RBCs count, Hb concentration, and PCV %; this may be due to cecal hemorrhages, bloody diarrhea, and loss of appetite, which leads to macrocytic hypochromic anemia (Hana et al., 2011). The reduction in Hb concentration may be attributed to hemorrhages in the caeca followed by the development of caecal lesions (Patra et al., 2010). According to Kavitha et al. (2018), on the other hand, the significant increase in total leucocytic count differential leucocytic count (monocytosis and eosinophilia) with a significant decrease in lymphocytes % confirmed by Bremner et al. (2021). The hematological parameters were markedly improved in the toltrazuril treated group (Harfoush et al., 2010; Youssef et al., 2015). Also, the hematological parameters in the cashew oil-treated group may be attributed to decreased cecal lesions and hemorrhage (Murakami et al., 2014; Sanches et al., 2019). Our study revealed a significant increase in serum creatinine, urea, uric acid, total bilirubin, ALT, AST and ALP in the infected non-treated group due to the harmful effect on liver function, liver injury and kidney parenchyma (Adamu et al., 2013). These findings are similar to those obtained by Harfoush et al. (2010), Mondal et al. (2011) and Patra et al. (2010). Moreover, Dovhiy et al. (2020) concluded that infestation with Eimeria showed a feasible increase in the total bilirubin content, urea, and AlAT enzyme activity. The current study revealed a significant decrease in serum cholesterol levels. The declined triglyceride levels may due to anorexia (Allen and McMurtry, 1984). There were no abnormalities in enzymes AST and ALT in the liver functions. These findings imply that cashews at the amounts tested were not harmful to laying hens (Braz et al., 2018). Flavonoids in cashew nut shell liquid can foster several mechanisms of action on lipid metabolism (Deszcz et al., 2000). Multiple pathological disorders are exacerbated by oxidative stress. Antioxidant defence mechanisms are reduced or absent in the presence of elevated levels of reactive oxygen species (ROS) (Heyman et al., 2011). Oxidative stress is a key player in a wide range of diseases. An increase in ROS or a decline in antioxidant defence mechanisms are two ways to describe it (Ighodaro and Akinloye, 2018). Various pathological disorders are exacerbated or even caused by oxidative stress. An increase in ROS or a decline in antioxidant defence mechanisms are two ways to define this phenomenon (Koinarski et al., 2005). Oxidative stress is a key player in a wide range of disease states. It is characterised by a rise in ROS and/or a decrease in antioxidant defence systems (Yusuf and Aliyu-Paiko, 2020), cashew nut shell liquid increased broiler chicks’ antioxidant enzyme activity. Cashew oil contains anacardic acid, which acts as coccistacis by blocking prooxidative enzymes involved in ROS generation (Green et al., 2007; Ha and Kubo, 2005; Sun et al., 2006), acting directly on the chelation of metallic acids but do not quench reactive oxygen species (Kubo et al., 2006; Tsujimoto et al., 2007), and preventing the generation of superoxide anion (Kubo et al., 2006). In the current study, contrast histopathological lesions revealed a heavy infestation of the intestinal crypts with different coccidial stages at 9th dpi and dead coccidial stages at 23rd dpi (Olabode et al., 2020). The trial boosted the cecal mucosa’s ability to heal. This may be due to the chronic stage, when the host can regulate its defensive mechanisms by boosting local mucosa immune responses (Khalafalla, 2009). The parasite’s life cycle in an infected host is self-limiting due to immunity from sickness and/or repeated infection. The groups treated with cashew oil or toltrazuril or cashew oil combined with toltrazuril showed a marked reduction of crypts affected which contained the parasite schizont and intestinal hyperplasia at 9th dpi, and slight hyperplasia of the lining crypts epithelium with mild interstitial mononuclear cells infiltration at 23rd dpi (Ashraf, 2011). Histopathological analysis of liver and kidney samples from the infected non-treated group exhibited necrotic foci associated with mononuclear cells infiltration 9th dpi, moderate hepatic fat vacuolation, and regenerating tubular basophilia 23th dpi., These findings match (Saber, 1995). Compared to the infected non-treated group, cashew oil, toltrazuril, and cashew oil combination improved livers and kidneys. Cashew oil and toltrazuril was best. At 9 dpi, cashew oil-treated mice had periportal eosinophilic cells and interstitial nephritis (mononuclear infiltration). 23 dpi, there was modest hepatic vacuolation and renal tubular epithelial degeneration. The toltrazuril-treated group had periportal esinophilic cell infiltration and significant degenerative and desquamative renal tubule alterations at 9 dpi, but normal hepatocytes, glomeruli, and tubules at 23 dpi. Cashew oil mixed with toltrazuril treated group showed slight mononuclear and esinophilic cells infiltration and mild hazy renal tubule enlargement with normal glomerulus at 9th dpi, and normal hepatocytes, renal glomeruli, and tubules at 23rd dpi.

Conclusion

Controlling avian coccidiosis requires collaborative efforts that will create new research avenues. Based on this study’s results, toltrazuril (control medicine) and cashew oil had the same anti-coccidial action. Toltrazuril and cashew oil worked best.

Acknowledgment

Authors would like to thank Deanship of Scientific Research, Taif University, Saudi Arabia for supporting this work.

IRB approval

The experimental methods, procedure were approved by the Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Ethical statement

The rules for the care and use of the animals used in the experiments were authorized by the Research Ethical Committee of the Faculty of Veterinary Medicine at the University of Kafr El-Sheikh, Egypt.

Statement of conflict of interest

The authors have declared no conflict of interest.

References

Abdisa, T., Hasen, R., Tagesu, T., Regea, G. and Tadese, G., 2019. Poultry coccidiosis and its prevention, control. J. Vet. Anim. Res., 1: 1-6.

Abreu, V.K.G., Pereira, A.L.F., de Freitas, E.R., Trevisan, M.T.S., da Costa, J.M.C. and de Melo Braz, N., 2017. Cashew nut shell liquid supplementation and the effect on lipid oxidation and color in fresh and spray-dried eggs. J. Fd. Proces. Preserv., 41: e13001. https://doi.org/10.1111/jfpp.13001

Adamu, M., Boonkaewwan, C., Gongruttananun, N. and Vongpakorn, M., 2013. Hematological, biochemical and histopathological changes caused by coccidiosis in chickens. Agric. Nat. Resours., 47: 238-246.

Allen, P. and McMurtry, J., 1984. Changes in pancreatic hormones associated with coccidiosis. Poult. Sci., 63: 1129-1135. https://doi.org/10.3382/ps.0631129

Ashraf, M., 2011. Evaluation of therapeutic and prophylactic potentials of toltrazuril against caecal coccidiosis of chickens in Bangladesh. PhD dissertation, M.Sc. thesis. Bangladesh Agricultural University, Mymensingh, Bangladesh

Braz, N., Freitas, E., Trevisan, M., do Nascimento, G., Salles, R., Cruz, C., Farias, N., da Silva, I. and Watanabe, P., 2018. Serum biochemical profile, enzymatic activity and lipid peroxidation in organs of laying hens fed diets containing cashew nut shell liquid. J. Anim. Physiol. Anim. Nutr., 102: 67-74. https://doi.org/10.1111/jpn.12659

Collares, L., Melo, A.D., Rodrigues, G., Papenborg, A., Cavenaghi, F., Joaquim, R. Ribeiro, A. and Moraes, P.D.O., 2018. Effect of functional oils on the excretion of oocysts and on the leukogram of broilers challenged by coccidiosis. In: 55a Reunião Anual da Sociedade Brasileira de Zootecnia, 28° Congresso Brasileiro de Zootecnia, Goiânia, Brasil, 27 a 30 de agosto de 2018. Sociedade Brasileira de Zootecnia-SBZ, Associação Brasileira dos Zootecnistas.

Craig, A.D., Khattak, F., Hastie, P., Bedford, M.R. and Olukosi, O.A., 2020. The similarity of the effect of carbohydrase or prebiotic supplementation in broilers aged 21 days, fed mixed cereal diets and challenged with coccidiosis infection. PLoS One, 15: e0229281.

Bremner, A., Kim, S., Morris, K.M., Nolan, M.J., Borowska, D., Wu, Z., Tomley, F., Blake, D.P., Hawken, R. and Kaiser, P., 2021. Kinetics of the cellular and transcriptomic response to eimeria maxima in relatively resistant and susceptible chicken lines. Front. Immunol., 12: 653085.

Davis, G., Siopes, T., Peiffer, R. and Cook, C., 1986. Morphologic changes induced by photoperiod in eyes of turkey poults. Am. J. Vet. Res., 47: 953-955.

Deszcz, L. and Kozubek, A., 2000. Higher cardol homologs (5-alkylresorcinols) in rye seedlings. Biochim. biophys. Acta Mol. Cell Biol. Lipids, 1483: 241-250. https://doi.org/10.1016/S1388-1981(99)00187-0

Dovhiy, Y., Kot, T., Rudik, O., Dovhiy, M. and Rudik, V., 2020. Changes in clinical characteristics and biochemical indices of fur animals and poultry blood under eimeria invasion. Colloquium-J. Голопристанський міськрайонний центр зайнятості, pp: 24-30.

Elkhouly, M., Khairy, M., Alim, A.E., Alim, A.E. and Ali, A., 2016. Effect of phytobiotics, probiotics and toltrazuril on chicken coccidiosis. Zagazig Vet. J., 44: 214-223. https://doi.org/10.21608/zvjz.2016.7875

El-Sawah, A., Aboelhadid, S., El-Nahass, E., Helal, H., Korany, A. and El-Ashram, S., 2020. Efficacy of probiotic Enterococcus faecium in combination with diclazuril against coccidiosis in experimentally infected broilers. J. appl. Microbiol., 129: 1020-1028. https://doi.org/10.1111/jam.14691

Farias, N.N.P., Freitas, E.R., Xavier, R.P.d.S., Braz, N.d.M., Souza, D.H. and Tavares, T.C.L., 2017. Cashew nut meal subjected to prolonged storage for quail feeding. Rev. Brasil. Zoot., 46: 576-583. https://doi.org/10.1590/s1806-92902017000700004

Ferket, P., Malheiros, R., Moraes, V., Ayoola, A., Barasch, I., Toomer, O. and Torrent, J., 2020. Effects of functional oils on the growth, carcass and meat characteristics, and intestinal morphology of commercial turkey toms. Poult. Sci., 99: 3752-3760. https://doi.org/10.1016/j.psj.2020.03.050

Green, I.R., Tocoli, F.E., Lee, S.H., Nihei, K.I. and Kubo, I., 2007. Molecular design of anti-MRSA agents based on the anacardic acid scaffold. Bioorg. med. Chem., 15: 6236-6241. https://doi.org/10.1016/j.bmc.2007.06.022

Ha, T.J. and Kubo, I., 2005. Lipoxygenase inhibitory activity of anacardic acids. J. Agric. Fd. Chem., 53: 4350-4354. https://doi.org/10.1021/jf048184e

Hana, A., Salasia, S., Mangkoewidjojo, S. and Kusindarto, D., 2011. Blood profile of rabbits infected with Eimeria magna. Anim. Prod., 13: 185-190.

Harfoush, M., Hegazy, A., Soliman, A. and Amer, S., 2010. Drug resistance evaluation of some commonly used anti-coccidial drugs in broiler chickens. J. Egypt. Soc. Parasitol., 40: 337-348.

Heyman, S.N., Rosen, S. and Rosenberger, C., 2011. A role for oxidative stress. Controv. Acute Kidn. Inj., 174: 138-148.

Ighodaro, O. and Akinloye, O., 2018. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the entire antioxidant defence grid. Alex. J. Med., 54: 287-293. https://doi.org/10.1016/j.ajme.2017.09.001

Itokawa, H., Totsuka, N., Nakahara, K., Maezuru, M., Takeya, K., Kondo, M., Inamatsu, M. and Morita, H., 1989. A quantitative structure-activity relationship for antitumor activity of long-chain phenols from Ginkgo biloba L. Chem. Pharma. Bull., 37: 1619-1621. https://doi.org/10.1248/cpb.37.1619

Kavitha, G., Giridhara, P., Byregowda, S., Sanganagouda, K. and Rajashekar, B., 2018. Diagnosis and management of caecal coccidiosis outbreak in a broiler farm. Intas Polivet, 19: 357-359.

Khalafalla, R.E.B.I., 2009. Evaluation of inhibition of Eimeria tenella sporozoites by antibody fragments expressed in pea. PhD thesis, University of Leipzig, Leipzig, Germany.

Koinarski, V., Georgieva, N., Gadjeva, V. and Petkov, P., 2005. Antioxidant status of broiler chickens, infected with Eimeria acervulina. Rev. Méd. Vét., 156: 498.

Kubo, I., Komatsu, S. and Ochi, M., 1986. Molluscicides from the cashew Anacardium occidentale and their large-scale isolation. J. Agric. Fd. Chem., 34: 970-973. https://doi.org/10.1021/jf00072a010

Kubo, I., Masuoka, N., Ha, T.J. and Tsujimoto, K., 2006. Antioxidant activity of anacardic acids. Fd. Chem., 99: 555-562. https://doi.org/10.1016/j.foodchem.2005.08.023

Kubo, I., Nihei, K.I. and Tsujimoto, K., 2003. Antibacterial action of anacardic acids against methicillin resistant Staphylococcus aureus (MRSA). J. Agric. Fd. Chem., 51: 7624-7628. https://doi.org/10.1021/jf034674f

Levine, N., 1988. The protozoan phylum Apicomplexa Volume 1. Chemical Rubber Company press. Inc., Boca Raton, Florida. USA.

Lillie, R.D., 1954. Histopathologic technic and practical histochemistry. The Blakiston Division, McGraw-Hill, New York, NY, USA

Mathis, G., Froyman, R., Irion, T. and Kennedy, T., 2003. Coccidiosis control with toltrazuril in conjunction with anticoccidial medicated or nonmedicated feed. Avian Dis., 47: 463-469. https://doi.org/10.1637/0005-2086(2003)047[0463:CCWTIC]2.0.CO;2

Mondal, D.K., Chattopadhyay, S., Batabyal, S., Bera, A.K. and Bhattacharya, D., 2011. Plasma biochemical indices at various stages of infection with a field isolate of Eimeria tenella in broiler chicken. Vet. World, 4: 404–409. https://doi.org/10.5455/vetworld.2011.404-409

Moraes, P. andretta, I., Cardinal, K., Ceron, M., Vilella, L., Borille, R., Frazzon, A., Frazzon, J., Santin, E. and Ribeiro, A., 2019. Effect of functional oils on the immune response of broilers challenged with Eimeria spp. Animal, 13: 2190-2198. https://doi.org/10.1017/S1751731119000600

Murakami, A., Eyng, C. and Torrent, J., 2014. Effects of functional oils on coccidiosis and apparent metabolizable energy in broiler chickens. Asian-Australas. J. Anim. Sci., 27: 981-989. https://doi.org/10.5713/ajas.2013.13449

Muroi, H. and Kubo, I., 1996. Antibacterial activity of anacardic acid and totarol, alone and in combination with methicillin, against methicillin resistant Staphylococcus aureus. J. appl. Bact., 80: 387-394. https://doi.org/10.1111/j.1365-2672.1996.tb03233.x

Olabode, V.B., Gunya, D.Y., Alsea, U.M., Choji, T.P.P. and Barde, I.J., 2020. Histopathological lesions of coccidiosis natural infestation in chickens. Asian J. Res. Anim. Vet. Sci., 5: 41-45.

Olanrewaju, C.A. and Agbor, R.Y., 2014. Prevalence of coccidiosis among poultry birds slaughtered at Gwagwalada main market, Abuja, FCT, Nigeria. Int. J. Eng. Sci., 3: 41-45.

Patra, G., Ali, M.A., Chanu, K.V., Jonathan, L., Joy, L., Prava, M., Ravindran, R., Das, G. and Devi, L.I., 2010. PCR based diagnosis of Eimeria tenella infection in broiler chicken. Int. J. Poult. Sci., 9: 813-818. https://doi.org/10.3923/ijps.2010.813.818

Quiroz-Castañeda, R.E., 2018. Avian coccidiosis, new strategies of treatment. In: Farm animals diseases, recent omic trends and new strategies of treatment (ed. R.E. Quiroz-Castañeda). IntechOpen, London. pp. 119. https://doi.org/10.5772/intechopen.74008

Saber, M., 1995. Pharmacological studies on some anticoccidial drugs in chickens. MVSc. thesis. Zagazig University, Egypt.

Sanches, L., Eyng, C., Garcia, R., Alves, G., Sangalli, G. and Nunes, R., 2019. Technical cashew nutshell liquid in diets of growing meat-type quails. Braz. J. Poult. Sci., 21: 1-6. https://doi.org/10.1590/1806-9061-2018-0823

Schalm, O.W., Jain, N.C. and Carroll, E.J., 1975. Veterinary hematology. Lea and Febiger.

Seddiek, S.A., Mobarak, M., Ali, M. and Metwaly, A., 2008. Potentiation of salinomycin anticoccidial effect with butylated hydroxy toluene (BHT) in broilers, fifth scientific conference. Suez Canal Vet. Med. J., pp. 241-258.

Soulsby, E.J.L., 1968. Helminths, arthropods and protozoa of domesticated animals. 7th ed. Bailliere Tindall, London, UK.

Soutter, F., Werling, D., Tomley, F.M. and Blake, D.P., 2020. Poultry coccidiosis: Design and interpretation of vaccine studies. Front. Vet. Sci., 7: 1-12. https://doi.org/10.3389/fvets.2020.00101

Sun, Y., Jiang, X., Chen, S. and Price, B.D., 2006. Inhibition of histone acetyltransferase activity by anacardic acid sensitizes tumor cells to ionizing radiation. FEBS Lett., 580: 4353-4356. https://doi.org/10.1016/j.febslet.2006.06.092

Toyomizu, M., Nakai, Y., Nakatsu, T. and Akiba, Y., 2003. Inhibitory effect of dietary anacardic acid supplementation on cecal lesion formation following chicken coccidial infection. Anim. Sci. J., 74: 105-109. https://doi.org/10.1046/j.1344-3941.2003.00094.x

Trevisan, M., Pfundstein, B., Haubner, R., Würtele, G., Spiegelhalder, B., Bartsch, H. and Owen, R., 2006. Characterization of alkyl phenols in cashew (Anacardium occidentale) products and assay of their antioxidant capacity. Fd. chem. Toxicol., 44: 188-197.

Tsujimoto, K., Hayashia, A., Ha, T.J. and Kubo, I., 2007. Anacardic acids and ferric ion chelation. Z. Naturforsch. C, 62: 710-716. https://doi.org/10.1515/znc-2007-9-1014

Xu, Z., Hu, C., Xia, M., Zhan, X. and Wang, M., 2003. Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult. Sci., 82: 1030-1036. https://doi.org/10.1093/ps/82.6.1030

Youssef, F., Abd El-Hamid, H.A. and El-Sheshtawy, E.A., 2015. Clinicopathological studies on the effect of Artemisia cina (Sheih Baladi) on coccidiosis in chickens. Egypt. J. Vet. Sci., 46: 11-24. https://doi.org/10.21608/ejvs.2015.800

Yusuf, H. and Aliyu-Paiko, M., 2020. Evaluation of cashew nut meal as phytobiotics in diet of broiler chickens and effects on feed efficiency, growth performance, blood metabolic and antioxidant profiles. Int. J. Vet. Sci. Anim. Husband., 5: 108-114.

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