Evaluation of the Immune Effect of Oral Sustained-Release Vaccine pcDNA3-3-1E-PLGA Microsphere of Eimeria acervulina in Chicken
Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*
1Faculty of Life Sciences, Tangshan Normal University, Thangshan, Hebei, 063001, China
2College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 071001, China
Fig. 1.
The tissue distribution of 3-1E gene in chicken in DNA microspheres vaccine group.
A-E represent PCR amplification of the 3-1E gene in chicken at 7th, 14th, 21st, 28th, 35th day after primary inoculation, respectively. Lane M, DL1000 DNA Marker; Lanes 1-8, show products of the gastric, duodenal, splenic, lung, heart, liver, intestinal sample and blank control, respectively.