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Evaluation of the Immune Effect of Oral Sustained-Release Vaccine pcDNA3-3-1E-PLGA Microsphere of Eimeria acervulina in Chicken

Evaluation of the Immune Effect of Oral Sustained-Release Vaccine pcDNA3-3-1E-PLGA Microsphere of Eimeria acervulina in Chicken

Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*

1Faculty of Life Sciences, Tangshan Normal University, Thangshan, Hebei, 063001, China
2College of Veterinary Medicine, Hebei Agricultural University, Baoding, Hebei, 071001, China
 
*      Corresponding author: [email protected]

Fig. 1.
The tissue distribution of 3-1E gene in chicken in DNA microspheres vaccine group. 
A-E represent PCR amplification of the 3-1E gene in chicken at 7th, 14th, 21st, 28th, 35th day after primary inoculation, respectively. Lane M, DL1000 DNA Marker; Lanes 1-8, show products of the gastric, duodenal, splenic, lung, heart, liver, intestinal sample and blank control, respectively.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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