Factors Affecting the Absorption of Exosomes by Sertoli Cells
Factors Affecting the Absorption of Exosomes by Sertoli Cells
Ma Hong*, Fu Bo, Wang Liang, Wang Fang, Guo Zhenhua and Liu Di*
ABSTRACT
Exosomes play an important role in the signal transmission process of Sertoli (ST) cells. However, what factors affect the ability of ST cells to take up exosomes remains unclear. To investigate the influences of time, temperature, cytochalasin D (Cyt D), and Fingolimod (FTY720) on exosome absorption by ST cellls, ST cells and exosomes were co-incubated under different environmental conditions. Then After exosome up taken by ST cells, cell surface fluorescence were observed and mean fluorescence intensity were measured. Furthermore, the cytoskeleton disruption and cell apoptosis after treatment by Cyt D and FTY720 treatment were detected, respectively. Our results showed that the time and temperature for co-incubation had a direct effect on the ability of ST cells to take up exosomes. Under our experimental conditions, co-incubation at 37°C for 4 hours gave maximum absorption, indicating that this was the optimal co-incubation condition. The Cyt D destroyed cytoskeletal structure of ST cells, while FTY720 induced ST cells apoptosis. With the increase of CYTD and FTY720 concentrations, the ability of ST to absorb exosomes is significantly reduced. It is concluded that the factors like time, temperature, Cyt D and TFY720 have important impacts on the ability of exosome absorption by ST cells, but different factors affect it in different ways.
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