Haemolymph Assay and Morphological Changes of Silkworm, Bombyx mori L. (Bombycidae: Lepidoptera) Infected with Bacillus thuringiensis var. sotto
Muhammad Zeeshan Nazar*, Ayyan Umer, Zahid Mahmood Sarwar, Faiz Ullah Faiz and Sikandar Hussain
Department of Entomology, Faculty of Agricultural Sciences and Technology, Bahauddin Zakariya University, Multan, Pakistan.
[email protected]
Figure 1
(a) Healthy and uninfected larva of B. mori. (b) Collecting of Haemolymph from caudal horn of B. mori. (c) Infected larva of 2nd day with light brown colour. (d) Larval color changed to coffee brown on 3rd day. (e) Yellowish body fluid discharged from the midgut of B. mori by rupturing the integument after highly infected with B. thuringiensis.
Figure 2
Concentration of total haemocyte count/ml after infection with B. thuringiensis var. sotto.