Identification and Antifungal Susceptibility Profile of Aspergillus niger Isolates from Chicken Blood in the Federal Capital Territory, Nigeria
Identification and Antifungal Susceptibility Profile of Aspergillus niger Isolates from Chicken Blood in the Federal Capital Territory, Nigeria
Bridget Maria Jessica Adah1, Samuel Mailafia1, H.O.K Olabode1, James Agbo Ameh1, Martha Echioda Ogbole1, Ebenezer Odey Odey1, Ifeanyi Cajetan Cashmir1, Hakeem Onigbanjo1, Monday Onakpa2, Enid Godwin3, Chinwe Elizabeth Okoli3, Nicodemus Nnabuike Mkpuma4, Oluwa Adikpe Agbonu5, Rabi Rebecca Mairabo6
ABSTRACT
Increasing incidence of invasive aspergillosis necessitated this study which aimed to isolate and identify Aspergillus niger in chicken blood from local markets within F.C.T, Nigeria. Three hundred and sixty (360) chicken blood samples were collected from six Area Councils (AC) in FCT. Isolation and preliminary identification were done using Potato Dextrose Agar (PDA) and Microscopy. Antifungal Susceptibility Testing (AFST) was done with liofilchem® antifungal disc using Disc Diffusion method by Kirby bauer and Ten (10) antifungal agents were tested. Fifty-four (54) isolates were confirmed positive for Aspergillus niger with prevalence of 16 (29.6%). The distribution of A, niger from the different Area councils were 8 (2.2%) from Abaji, AMAC 5 (1.4%), Bwari 6 (1.7%), Gwagwalada, 21 (5.8%), Kuje 10 (2.8%) and Kwali A4 (1.1%) bringing the overall prevalence to 15%. Isolates showed 100% susceptibility to Posaconazole, Variconazole, Nystatin, Ketoconazole and Itraconazole while multiple drug resistance was observed in routinely used antifungal agents like Metronidazole, Fluconazole, Capsofugin, Amphotericin B, and Griseofulvin with a MARI of greater than 0.2 from all antifungal agents tested which was significant indicating that isolates were all multi drug resistance organisms. This study discovered the presence of A. niger from chicken blood in the Federal Capital Territory which is a first-time report in Nigeria and hence there is need for isolation and invivo AFST studies with regards to other animal species in Nigeria, as this will guide clinicians in antifungal agents’ selection towards achieving effective therapy as a control strategy for eradicating the disease.
Keywords | Aspergillus niger, MARI, Zoonotic, Antifungal agents, Potato dextrose agar, Aspergillosis
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September 2024
Vol. 12, Iss. 3
