Impact of Ocimum basilicum Essential Oils Supplementation in Semen Extenders on the Quality of Cryopreserved Canine Sperm
Impact of Ocimum basilicum Essential Oils Supplementation in Semen Extenders on the Quality of Cryopreserved Canine Sperm
Nguyen Van Vui*, Nguyen Luu Trong Nghia, Huynh Thanh Tan
ABSTRACT
During cryopreservation, canine sperm faces a significant challenge: oxidative stress, which leads to reduced sperm quality. This study was conducted to evaluate the effects of Ocimum basilicum essential oils supplementation in the dilution medium on the quality of canine sperm frozen at -196℃. Sperm quality was assessed based on progressive motility, viability, membrane integrity, and oxidative resistance. The results showed that sperm quality improved at lower concentrations of essential oils (below 25 µg/ml) and decreased at concentration of 25 µg/ml. Notably, the essential oils concentration of 20 µg/ml yielded the best sperm quality, with significant differences compared to other levels (P<0.05). The study concludes that Ocimum basilicum essential oils affects canine sperm quality in a concentration-dependent manner, with 20 µg/ml being the optimal concentration to reduce oxidative stress and enhance sperm quality during cryopreservation.
Keywords | Canine sperm, Frozen-thawed, Ocimum basilicum, Essential oils, Antioxidants, Cryopreservation
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