Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
...ahexaenoic acid (DHA) on frozen-thawed quality of bull semen. Twenty-four ejaculates (8 from each bull) were collected from three bulls. Semen ejaculates having motility ≥70% and normal morphology ≥80% were extended into Tris extender consist of equal concentration of each ALA and DHA (0, 3, 5, 10,15ng/ml). Extended semen was cooled at 5oC for 2 hours at 5oC and then packaged in 0.25ml straws and stored into liquid nitrogen for 24 h...
Alicja Kowalczyk1, Marian Kuczaj2, Anna Szul3 and Ewa Czerniawska-Piątkowska4*
...etate) on the quality of frozen-thawed bovine spermatozoa. In this study, ten Polish Holstein-Friesian bulls with an average age of 3.5 ± 0.5 years were housed individually in pens. From each bull, two ejaculates were collected using an artificial vagina. Semen samples that showed more than 60% motility and 60% viability were selected for this experiment. To the four diluted samples were added 0µg/mL successively; 2µg/mL; 4µg/mL and 8&...
Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...ris based) and number of frozen-thawed ram sperm (100 × 106 and 50 × 106 sperm dose) on pregnancy and lambing rate following laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assigned to one of four groups; G1 (n = 18; commercial Triladyl®/ 100 &time...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...TCA extender ameliorated frozen-thawed quality of beetal buck spermatozoa.
... 
Asmatullah Kaka1*, Wahid Haron2, Nurhusien Yimer3, Abdullah Channo1, Ali Raza Jahejo2, Mahdi Ebrahimi3, Dildar Hussain Kalhoro4
...to depict the quality of frozen-thawed bull sperm extended in Tris-buffered extender (TBE) supplemented with docosahexaenoic acid (DHA). Electro ejaculator was used to collect the semen and ejaculates having ≥ 20 × 109 spermatozoa with an initial motility rate above 70 %, and normal morphology and viability of ≥ 80 % were extended in TBE, to which different concentrations (0, 3, 5, 10 and 15 ng/ml) of DHA were enriched. Semen sample with supplemen...
Nguyen Van Vui*, Nguyen Luu Trong Nghia, Huynh Thanh Tan
Nguyen Van Vui*, Huynh Thanh Tan
...ro;g/ml) in extenders on frozen-thawed sperm. The oil was extracted via steam distillation and sperm quality was assessed by motility, viability, membrane integrity and lipid peroxidation. The results indicated that concentrations below 25 µg/ml improved sperm quality, while 30 µg/ml had a negative impact. The 25 µg/ml extender showed the best sperm quality (P<0.05). In conclusion, Perilla frutescens essential oil positively affects sperm ...
