Submit or Track your Manuscript LOG-IN

Effect of Glutathione and L-Cysteine Supplementation on the Quality of Frozen Beetal Buck Spermatozoa

Effect of Glutathione and L-Cysteine Supplementation on the Quality of Frozen Beetal Buck Spermatozoa

Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider

Animal Reproduction and Genetics Program, Animal Sciences Institute, National Agricultural Research Centre, PARC, Islamabad 44000

 
* Corresponding author: [email protected]

ABSTRACT

This study was designed to assess the cryosurvival of beetal buck spermatozoa with the supplementation of Glutathione (GLU) and L-cysteine (CYS) in the extender. In the first experiment, semen samples from each of the five mature bucks were pooled and diluted in Tris-citric acid (TCA) extender containing Glutathione (0.5mM, 1mM), L-cysteine (5mM CYS, 10mM CYS), and control. In experiment 2, semen samples (n=5) were diluted in TCA extender containing differential doses of combined antioxidants (0.5 mM GLU+ 5mM CYS, 0.5mM + 10mM CYS, 1mM GLU + 5mM CYS, 1mM GLU + 10mM CYS and Control). At post-thawing, progressive motility, plasma membrane integrity (PMI), and livability of buck spermatozoa were higher (P< 0.05) with 1mM GLU and 10mM CYS as compared to control. In the second experiment, post-thaw progressive motility, PMI, and livability were higher (P< 0.05) with 1mM GLU + 10mM CYS and 1mM GLU + 5mM CYS compared to other doses of GLU+CYS and control. We concluded that optimized supplementation of Glutathione and L-cysteine in TCA extender ameliorated frozen-thawed quality of beetal buck spermatozoa.

To share on other social networks, click on any share button. What are these?

Pakistan Journal of Zoology

December

Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2501-3000

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe