Effects of Shenling Baizhu Powder on the Proliferation and Invasion of Tumor Cells: Regulating Polarization of Macrophages in Co-Culture System
Effects of Shenling Baizhu Powder on the Proliferation and Invasion of Tumor Cells: Regulating Polarization of Macrophages in Co-Culture System
Wei Tian, Junquan Han*, Wenheng Han, Jia Wei and Hong Wang
ABSTRACT
The objective of this study was to explore the effects of Shenling Baizhu powder on the proliferation and invasion of tumor cells by regulating macrophage polarization in the co-culture system. Raw264.7 macrophages were added with IL-424h to lead M2-like macrophages, and the activation phenotype was identified. The Lewis lung cancer (LLC) mouse lung cancer cell line was used for experimental studies. The cultured cells were separated into six groups. RT-PCR was used to test the mRNA expression of iNOS and M2 type I arginase (ArgI) genes in M1 macrophages at 12h, 24h, 36h, and 48h. Using Elisa technology, the contents of cytokines IFN-γ and TNF-α in the co-culture supernatant were tested at 12h, 24h, 36h, and 48h. RT-PCR and Western blot were used to test PD-L1 mRNA and protein expression in lung cancer tumor cells of LCC mice. CCK-8 method was used to test the multiplication of LCC mouse lung cancer tumor cells, and flow cytometry was used to test cell apoptosis. Transwell chamber was used to test cell invasion ability. The cell viability of the co-cultured drug-added group was significantly lower than the co-cultured blank group at 12h, 24h, and 48h. Cell apoptosis in the co-cultured drug group was significantly higher than the co-cultured blank group at both 12h and 24h. The number of cell invasion in the co-culture blank group increased compared to the LLC blank group and LLC drug-added group, while the co-culture drug-added group showed reduction in the number of cell invasion compared to the co-culture blank group. Alteration in macrophage phenotype greatly hampers the growth and infiltration of lung cancer cells. The discovery not only enhances our comprehension of the functioning of Shenling Baizhu Powder, but also presents novel molecular indicators and possible treatment objectives.
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