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Aiza Tahir, Muhammad Asif, Zaigham Abbas and Shafiq ur Rehman*

...t in milk indicated that proliferation of S. aureus was successfully ceased until six hours post infection when applied at and MOI of 100.

...
Pinar Oztopcu-Vatan1, Gokhan Kus2, Emine Inan3, Melek Gunindi Korkut3, Selda Kabadere4,* and Ruhi Uyar4
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2
...mphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked immuno sorbent assay (ELISA) in 2015 and 2016. The diagnosis of avian leukosis virus subgroup A (ALV-A) infection was confirmed by necropsy, hist...

Zahir Shah, Ahmed Nawaz Tunio, Shakoor Ahmad, Ijaz Ahmad, Javid Ali, Sher Bhader Khan

...moved with care to avoid proliferation of uterine contents into the peritoneal cavity. Penicillin powder was dusted into the surroundings area of the uterus. The cow was treated with antibiotics, analgesics and liver tonics during the first week post-surgery. Local antiseptic dressing was performed using povidone-iodine daily for one week and skin sutures were removed at 14 day post-operation.

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Sitansu Pan and Amrita Das

Population proliferation and spread of Trichoderma spp. in soil under two different delivery systems
...t application population proliferation (build-up) and spread of Trichoderma spp., Trichoderma harzianum and Trichoderma viride in three different types (sterilized, solarised and natural) clay loam soil at 50% water holding capacity (pH 6.5) was studied both under laboratory and field conditions following two types of delivery systems, through organic based (vermicompost + neem cake (90% + 10% w/w)) and seed based (sterilized wheat seed) inocula. The populatio...
Beenish Zahid1,*, Asim Aslam2, Zafar Iqbal Chaudhry2 and Raheela Akhtar2
...ation and fibrous tissue proliferation at medullar region of thymus and bursa were observed. These results indicated that pathogenic field IBDV caused the serum biochemical changes by damaging the liver and kidney tissues with histopathological effect on lymphoid organs of chicken.
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Sanjukta Chakrabarti1, Colin J. Barrow2, Rupinder K. Kanwar3, Venkata Ramana1*, Rakesh N. Veedu4,5 and Jagat R. Kanwar3* 

...tors (VEGFRs). Survival, proliferation, migration and invasion of endothelial cells which give rise to the vascular network, is mediated through VEGF/VEGFR activation of the signal transduction pathways. Thus blocking VEGF is an efficient strategy for blocking tumor angiogenesis. Several anti-VEGF drugs have been developed over the last two decades and some are still at different development stages of pre-clinical and clinical trials. In this study, we explore...
Zhenyu Chang1, Hui Zhang1, Khalid Mehmood1,2, Mujeeb Ur Rehman1, Xiaodan Yuan1, Zhaoyang Li1, Fazul Nabi1, Xiaoxing Wu1, Xinxin Tian1, Xueting Liu1, Jingen Xu3 and Donghai Zhou1,*
...nt human (rh) TFF3. Cell proliferation and TFF3 mRNA expression were examined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays and real-time quantitative PCR, respectively. The results showed that rhTFF3 effectively prevented the cell injury induced by LPS (P<0.05). A low concentration of LPS (10 µg/mL) down-regulated the expression of TFF3 mRNA, whereas a high concentration of LPS (100 µg/mL) up-regulated TF...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
... which can regulate cell proliferation, differentiation, apoptosis, and involve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analys...
Sumaira Zareen1, Syeda Sadaf Zahra2, Ayeza Mehmood3, Muhammad Asadullah4* and Aish Muhammad5

 

...yric acid (IBA) for root proliferation. Best shoot proliferation (4.48 per explant) was observed in MS medium containing 0.2/0.4 mg/L BAP/IAA combination. For rooting of the micro shoots, MS medium was supplemented with 1.0 mg/l IBA exhibited best results with 4.69 mean value of roots per shoot at an average root length of 3.86 cm. After acclimatization and transplantation, 90% of the In- vitro derived plants were found heal...

 Sadia Tehrim*, M. Yasin Mirza** and G. Mustafa Sajid*

COMPARATIVE STUDY OF DIFFERENT GROWTH REGULATORS FOR EFFICIENT PLANT REGENERATION IN GRAPES
... Additionally, the shoot proliferation response to increasing level of BAP was found genotype dependent. Naphthaleneacetic acid (NAA) also showed a positive effect on in vitro growth responses (shoot proliferation and root induction) when combined with BAP. The media containing NAA, BAP and kinetin accelerated the growth in terms of shoot mass in two accessions, 4494(1) Kowar Sufaid and 4472(12) Harker Agoon and again the cu...
Muhammad Shahbaz Aslam1, Iram Gull1, Zaigham Abbas2,* and Muhammad Amin Athar1
...lpha;1 demonstrated anti-proliferation activity as assessed by MTT assay. This study also showed that N-terminal fusion of SUMO withIFNα2-Tα1 is effective for its soluble expression and to make its purification process more convenient.
...
Guifen Liu1,2, Xiaomu Liu1,2, Khuram Shahzad3, Wei You1,2, Juan J. Loor3,* and Fachun Wan1,2,4,*
...to disease, cell growth, proliferation and lipid metabolism, including PPAR, are important during development of the adipose tissue. These data indicate that future research should emphasize the study of key genes related to synthesis, metabolism, and growth of adipose tissue during development. This will allow to determine the functional relevance of the genes and pathways uncovered.
...
Ayesha Noreen1, Dilara A. Bukhari2 and Abdul Rehman1,*
...pathways, promoting cell proliferation, invasion, inducing angiogenesis, inflammation and cellular transformation in cancer. A better understanding into the mechanism of ROS in cancer progression might be useful for the development of biomarkers and therapeutic strategies. The objective of this review was to summarize the roles of ROS in different stages of cancer, cell invasion, angiogenesis and metastasis.
...
Muhammad Khan1,*, Amara Maryam2, Hafiz Abdullah Shakir1, Javed Iqbal Qazi1 and Yongming Li3,*
...on without enhancing the proliferation of cells and BHS-I was equally toxic to both BaP-treated and untreated A549 cells. Taken together, the data of present study indicate that BHS-I not only inhibits metastasis but also induces cell death in BaP-treated A549 cells. Therefore, BHS-I may become a potential lead compound for treatment of lung adenocarcinoma.
...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...ed microscope, and MDSCs proliferation pattern were determined through growth curve analyses. MDSCs were identified by immunofluorescence and RT-PCR, and the immunofluorescence antibody invoved Sca-1, CD34, CD144, Desmin and CD45 which are the makers of MDSCs. Finally, MDSCs were induced into Adipocytes, osteoblasts, chondrocytes and neuron-like cells by the optimized inducing medium. After the two-step digestion and differential attachment technique, high pur...

Mehboob Arslan* and Xin Yang 

.... For absolute survival, proliferation, and propagation inside the host cell, viruses modulate certain metabolic pathways to destabilize the host metabolism. These alterations in host metabolic signaling as a result of virus-host interaction generate a favorable environment for the viral pathogens. This phenomenon is a hotspot for investigations in order to improve the diagnosis and drug development of future antiviral therapeutics. In fact, metabolism is cons...
Tian-Yi Zhao1, Zi-Qing Liu2, Bo Yang1, Qiao Gao1, Hong-Yu Zhang1, Pei-Yu He1, Ming-Hua Duan1* and Yu-Li Yan1*
...APs promoted erythrocyte proliferation and increased hemoglobin content. The possible mechanism is by increasing Cyclin D1, Stat5 and Bcl-2 protein expression.
...
Xiaopeng Tang1, Wen-qin Su1 and Re-jun Fang1,2,*
... calcitonin (CT) on cell proliferation, NaPi-IIb expression, and the absorption of phosphorus in porcine intestinal epithelial cells (IPEC-1). The experiment consisted of 1 control treatment and 5 levels of CT treatments (1×10-12 to 1×10-8 mol/L), and each treatment has 6 replications. Cell proliferation was determined by the MTT method. Cell morphology and phosphorus concentration in the ce...
Qiuxia Lei1, 2, Haixia Han1, 2, Jinbo Gao1, 2, Yan Zhou1, 2, Wei Liu1, 2, Chunlei Wang3, Jie Liu1, 2, Dingguo Cao1, 2, Huimin Li1 , Baohua Huang1,* and Fuwei Li1, 2, *  
...a improved the microbial proliferation and shortened the time that the substrate reached to the fermentation temperature, resulting in the retention of N, P, K and the reduction of the NH3, H2S from the fermentation litter.
...
Zijuan Li1,2, Rong Ma2, Muhammad Khan3*, Chenchen Liu2, Xiaolin Cui2 and Yongming Li1,2*
...sarcoma U2OS cells. Cell proliferation was measured using CCK-8 assay and colony forming assay. Flow cytometry was used to determine apoptosis and cell cycle profile. Western blot was used to measure the expression of various proteins. The data demonstrated that piceatannol inhibited growth and induced G2/M phase arrest and apoptosis. Further studies showed that piceatannol induces mitochondrial apoptosis as shown by Bcl-2 family proteins modulation. Finally, ...

 Li-na Li1, Sheng Li2, Ping Gui3, Jing-hui Li1, Fen Ai4,* and Li-li Cai1,*

...CCM inhibited MRC-5 cell proliferation and reduced collagen deposition as measured by CCK-8 assay, Sircol assay, qPCR and western blot. MSCCM also reduced the expression of the pro-fibrotic TGF-β1 and increased the expression of anti-fibrotic IFN-γ at both mRNA and protein levels. Furthermore, we found that MSCCM inhibited the activation of NF-κB signaling pathway induced by irradiation as well. Our data suggest that MSCCM could reduce irradia...
Lin Sun1, Xiaofang Bu2, Jian Wang1*, Xiaorui Liu1 and Zhanyi Kong2
...athway of inhibiting the proliferation of MP.
...
Rimsha Munir1, 2*

 

...ased or increased– proliferation rates when cultivated as 3D tumor spheroids. The presented work aimed to study the interplay between cell proliferation rates and metabolic stress in 2D and 3D cell culture systems. Two cell line models (HepG2; liver cancer cell line and HCT-116; colorectal cancer cell line) were selected that showed inverse proliferation trends under 2D and 3D cell c...
Tian-Yi Zhao1, Zi-Qing Liu2, Shu-Fei Ma3, Bo-Yang1, Fan-Fan Guo1 and Ming-Hua Duan1*

 

...ractylolide on PC12 cell proliferation, growth inhibition rate and apoptosis. In order to assess the spatial learning and memory abilities of AD rats, Morris water maze model was applied in vivo, and the activity of the NF-κB signaling pathway and concentrations of TNF-α, IL-6, and IL-1β were measured. The results demonstrated that biatractylolide can reduce apoptosis in hippocampal cells, prevent and improve the cognitive decline induced by A...
Larysa Kladnytska1, Anatoliy Mazurkevych1, Natalia Bezdieniezhnykh2Oleg Melnyc1, Sergiy Velychko3, Mykola Malyuk1, Vasyl Danilov1Yuriy Kharkevych1 and Magdalena Gryzinska4*
...termination the index of proliferationdogs adipose-derived stem cells (DADSCs) on IVth and Xth passages. Established that DADSCs contains multipotent stem cells, that are characterized by an almost homogenous fibroblast-like cells оn the IVth and Хth passages. It was difined, that fibroblast-like cells оn the IVth and Хth passages was expressed cytoplasmic and membrane proteins, which are sp...

Mohammad Aslam Khan*, Samiullah* and Zulfiqar Ali*

DYNAMICS OF URBAN SPATIAL GROWTH AND PLANNING: A CASE STUDY OF PESHAWAR
...le to control their proliferation for sustainable development of the city in future.
...
Feng-Mei Yang1, Rui Li2, Xiu-Xue Hu3, Yong Liang4, Bo Gao3* and Wei Chen3*
...) and western blot; cell proliferation inhibition rates (CPIR) of HSF were obtained by MTT, and HSF apoptosis was detected using Flow Cytometry (FCM). UVB irradiations significantly inhibit HSF growth, showing positive correlation with the increasing of CPIRs and apoptotic rates; The relative expression levels of β-catenin and Senescence marker protein-30 (SMP30) gene in UVB treated group were significantly lower than that of the control group; Gene expre...
Aribah Naz1*, Uzma Badar1,Afsheen Arif2*, Zaid Qureshi1, Sondas Saeed1,Erum Shoeb1, Maqsood Ali Ansari1 and Obaid Yusuf Khan1
... stability, survival and proliferation of species in their respective habitat.
...
Yujun Zhao and Jianping Fan*
... without medication, the proliferation inhibition rate of cells processed with Kanglaite injection (10, 20 and 40 μL/mL) significantly increased (p <0.05), the apoptosis rate increased (p <0.05), the expression of Bcl-2 protein decreased (p <0.05), the expression of Bax protein significantly increased (p <0.05), and the activity of Caspase-3 and Caspase-8 increased (p <0.05) in a dose-dependent manner. Hence...
Shikang Deng1,2, Yan Jin2, Jing Xu2, Xiufang Zhu2, Pinghai Hu2, Jiao Li2, Li Zhang2 and Jianzhong Tang2,*
... used to detect the cell proliferation level of each group; Western blot was used to detect the expression of TGF-β1, cyclinD1, and CDK4 protein in each group of cells; and real-time polymerase chain reaction was used to detect the expression levels of TGF-β1, cyclinD1, and CDK4 mRNA in each group of cells. The results showed that after 5-FU intervention, the proliferation of bile duct scar fibroblasts was inhibite...

Inam Ul Haq1*, Humara Umar2, Naeem Akhtar3, Muhammad Azhar Iqbal2 and Muhammad Ijaz1

Techniques for Micropropagation of Olive (Olea europaea L.): A Systematic Review
...is technique is used for proliferation of olive (Olea europaea L.) tree which is globally cultivated for centuries and its value added products are consumed worldwide. The traditional propagation techniques of olive are uneconomical with wastage of time while micropropagation of olive may expedite the development of important characteristics like biotic and abiotic resistance with high yield and premium quality olive oil. However, the commercial micropropagati...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
... and are also capable of proliferation and differentiation, ensuring the efficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then ...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...on, degradation and cell proliferation.
...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...The relationship of cell proliferation and apoptosis is important in normal kidney development. The ZFX gene is important for regulation of growth, proliferation and differentiation in tissue cells while the Bcl-2 and BAX genes regulate apoptosis. This experiment adopted the real-time fluorescence quantitative technique to test ZFX, Bcl-2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after...

Mai A. Kilany1; Hanaa H.A. Gomaa1; Yousry E. Abo El-magd2 and Nermin Raafat2.

...tocytes leading to their proliferation and triggers HCC. Manganese superoxide dismutase (MnSOD) is a nuclear-encoded antioxidant enzyme that neutralizes free radicals. Val16Ala single nucleotide polymorphism (SNP) is one of the most widely investigated SNPs in the MnSOD gene, where C to T transition resulting in the con-version of valine to alanine at amino acid 16 within the mitochondrial signaling se-quence. This study was designed to investigate the associa...

Shimaa M. Gad1, Ahmed A. Kheder1, Mohamed A. Awad 2

...ing phyllody, yellowing, proliferation, virescence and little leaf symptoms
was observed Giza, Egypt.
Objective: The current work aims the detection and molecular identification of phytoplasma infecting
Gazania in Egypt.
Methods: Phytoplasma disease was detected and isolated from naturally infected gazania plants during
surveys in flower nurseries and open filed in Giza governorate, it was transmitted...

Abdelaziz A. Yassin, Ayman H. El Deeb, Abeer E. Mansour, Hussein A. Hussein

...vaccination. Lymphocytic proliferation assays demonstrated the continuous response of lymphocytes started at the 3rd day till reaches peak at 28th day post vaccination then start to decline. Whereas the peak titers for antibodies were reported at the 28th day post vaccination. Indeed, the study reports the role of ail adjuvant in stimulating the cellular response for inactivated vaccine.

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Bingjie Zhou1, Hitesh Bhagavanbhai Mangukiya1, Siva Bharath Merugu1, Fakhar-un-Nisa Yunus1, Yuchen Fan1, Zhenghua Wu1,* and Dawei Li1,2,*
... AGR2 was tested by cell proliferation and wound healing assays, while the auto-fluorescent property of DsRed was confirmed by fluorescence microscopy and immunofluorescence. The potential application of his-AGR2-DsRed was demonstrated by its internalization in fibroblasts. Therefore, the purified recombinant fluorescent protein would be a very useful tool for further investigation of AGR2’s extracellular function and molecular mechanism.
...
Kanwal Haneef1,2, Rakhshinda Habib1,3, Nadia Naeem1,3 and Asmat Salim1,*
...their ease of isolation, proliferation, anti-apoptotic and proangiogenic properties. MSCs have the ability to fuse with somatic cells in vivo and depending upon the microenvironment, the fusion products may perform tissue-specific function. Stem cell factor (SCF) is one of the important cytokine for cardiomyocyte survival following cardiac injury. The main objective of the present study is to determine the fusion potential of SCF transfected MSCs and ca...
Junli Liu1, Tongfang Yang2 and Lisheng Wu3*
... of homoharringtonine on proliferation and apoptosis of osteosarcoma cells through long non-coding RNA (lncRNA) LINC00857/microRNA (miR) -340. U2OS of osteosarcoma cells was treated with harringtonine at a concentration of 0.25, 0.5, 1, 2, 4 μg/ml. P21 and cysteine-containing aspartic protease 3 (Caspase-3) protein expression in U2OS cells were detected by western blot, thiazole blue (MTT) and plate cloning experiments to determine cell survival and colony ...

Jiao Ma*

...d and studied. Increased proliferation and differentiation of these cells in areas of cardiac ischemia is a significant factor in the repair and enhancement of heart injury. This role of endogenic cardiac stem cells can be regulated by factors. Numerous causes, such as paracrine and autocrine factors, extracellular matrixes and genetic factors. In general, it is well known that these variables could have an appropriate role to improve the effectiveness of the ...

Dabeeran Zehra1, Zahida Memon1, Shumaila Usman2,*, Almas Jabeen3, Kauser Ismail1 and Rehan Ahmed Siddiqui2

...associated with cellular proliferation, however the effects on cellular apoptosis were found to be insignificant.

...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...increase in cell growth, proliferation and mineralization markers of osteoblast cells that increased with time as the highest concentrations of CeO2 innanobioactive glass (sample CL/CH/C10). Cell viability proved also that all scaffolds and their extracts showed proliferation inhibition with time < 25% reference to final cell number of control cells. Among the composites, having CL/CH/C5 showed the highest...

Sheng Dong1*, Ning Li2, Jiawei Zhang2 and Ting Wang2

...scurainia sophia (DS) on proliferation, apoptosis and endoplasmic reticulum stress of high glucose (HG)-induced mouse podocytes and its regulation of LncRNA KCNQ1OT1. In vitro cultured immortal mouse podocytes were divided into Con group, HG group, HG+DS-L group, HG+DS-M group, HG+DS-H group. si-NC and si-KCNQ1OT1 were transfected respectively into podocytes, followed by 30 mmol/L glucose intervention for 24 h. pcDNA, pcDNA-KCNQ1OT1 were transfected into podoc...

Xian Zhang1, Erjiang Lin1, Shizhe Hong1 and Zhengjie Zhu2*

...ransferase (SSAT) on the proliferation and apoptosis of prostate cancer LNCaP cells under hypoxic conditions. Prostate cancer LNCaP cells were transfected with the eukaryotic expression plasmid pcDNA3.1 (D)/V5-His-SSAT, and cultured at 1% oxygen concentration. Western blotting was used to detect expression of SSAT, Akt, GSK-3β and β-catenin proteins; and flow cytometry was used to detect cell apoptosis rate and mortality rate. SSAT was successfully t...

Wei Cui1, Yanwei Du1, Lijuan Jiang1, Yan Wei1, Yuguo Li1, Wenfeng Zhang1* and Ling Zhang2*

...el was established, cell proliferation and apoptosis were detected. The protein expression levels were detected by western blot. Rats with type 2 diabetes mellitus were fed high-fat-sugar diet and low dose of streptozotocin for 4 weeks to observe the body weight, fasting blood glucose, etc. The pathological changes of hippocampal CA1 region were observed through hematoxylin-eosin staining, and the expression of related proteins was detected. The results showed...

Feng Xu, Weijing Fan and Guobin Liu*

... rutin on ox-LDL-induced proliferation, apoptosis and oxidative stress of vascular endothelial cells. It is concluded that rutin had a protective effect on ox-LDL-induced vascular endothelial apoptosis and oxidative injury, and its mechanism may be related to the up-regulation ofNEXN-AS1/miR-410-3p pathway.
...
Noha M El-Motaily1, Ossama M Abdou1, Heba S Farag1, Kawkab A Ahmed2, Mahmoud Saber1*
... and caused by excessive proliferation of the normal skin commensal Demodex mites. Hemato-biochemical changes were previously reported in patient dogs with demodicosis due to the effect of stress caused by mites. This study was conducted on 38 dogs, 20 of them were used as control group and 18 were demodicosis infected dogs, for evaluation of hematological, biochemical and histopathological alterations. Beside detection of the antioxidant trace elements (Zn, C...

Rizwana Abdul Ghaffar* and Kamil Nadeem

...ecrosis and dilation and proliferation of blood vessels.

...

Xiaoguang Su1,*, Yanjun Gao2, Yanling Wang1 and Yaohui Ma2

...the effect of HAE on the proliferation and apoptosis of keloid fibroblasts and its mechanism. Primary HFB cells were isolated and cultured, and treated with different concentrations of HAE for 48 h. Cell viability was measured by MTT. Flow cytometry was used to detect the apoptosis rate. qRT-PCR and Western blot were used to detect the effects of HAE on miR-128-3p and EPHB2. The above method was used to detect cell viability and apoptosis rate after overexpres...

Guang Yin, Wencheng Kong, Yuqiang Shan, Jian Zhang, Rongchao Ying and Sixin Zheng*

...t of LncRNA TINCR on the proliferation, invasion, migration and chemotherapy sensitivity of hepatocellular carcinoma Hep-3B cells and its mechanism. In present study Hep-3B cells cultured in vitro were divided into control group (normal culture), NC-siRNA group (transfected with NC-siRNA), and TINCR-siRNA group (transfected with TINCR-siRNA), In addition, cells treated with cisplatin after transfection of TINCR-siRNA were designated as the TINCR-siRNA + cispla...

Mehroze Amin1, Afifa Yaqub1, Qindeel Fatima1, Rabail Hassan Toor1,3 and Abdul Rauf Shakoori1,2*

... of the cytotoxicity and proliferation parameters showed that higher concentrations of metformin were cytotoxic for the breast cancer cells with EC50 of 22.75µM. Analysis of expression of p53 and the interacting partner genes showed that metformin upregulated p53 expression in a dose-dependent manner, while the expression of interacting partners is downregulated indicating that metformin exhibits the anti-cancerous effect by regulating the p53 pathway. A...
Afifa Yaqub1, Mehroze Amin1, Qindeel Fatima1, Rabail Hassan Toor1,3, Saira Aftab1 and Abdul Rauf Shakoori1,2*
.... Analysis of growth and proliferation parameters by various cell-based assays such as neutral red assay and BrdU cell proliferation assay showed that higher concentrations were lethal for the cancerous cells. IC50 was found to be 12.6 µM. Further contextualization of the therapeutic potential of G9a inhibition was provided by expression analysis of the G9a gene and genes associated with cell p...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang*, Jinhua Chen and Miao Tian

...and HDAC8 inhibiting the proliferation, metastasis and invasion of breast cancer cells. Tissue samples (28) were collected, and malignant and normal tissues were confirmed pathologically. The breast cancer cell line MDAMB-231 and the immortalized normal breast epithelial cell line MCF-10A were used in this study. Western blot analysis of miR-216b-5p and HDAC8 protein expression in tissue samples; reverse transcription quantitative PCR (RT-qPCR) real-time analy...

Xiajun Zhang1, Jie Yang2, Wenjun Zhou1, Zhenshi Chen2, Weidong Wu3, Shaoru Zhang2 and Lihui Wang2*

...GF-β regulates cell proliferation, angiogenesis and metastasis, and is an inducer of epithelial-mesenchymal transformation. Tumor cells activate the TGF-β/Smad pathway, and inhibiting its signal transduction pathway is an important strategy for tumor therapy. First, we have found lncRNA CASC7 is a poor prognostic marker of endometrial cancer. Then, ASPN was specifically expressed in endometrium and cell specific expression in basophils, among the mos...

Qingyue Han1, Ying Li1, Jichang Deng1, Kunxuan Huang1, Yanyang Yang1, Quanwei Li1, Zhuowei Zhang1, Na Qiao1, Yanju Ji2, Khalid Mehmood3, Sarfaraz Ali Fazlani4, Hui Zhang1 and Zhaoxin Tang1*

...way and myocardial cells proliferation.

...

Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1 

... of the gene expression, proliferation, and differentiation of the recipient cells. Methodology: 24 male New Zealand albino rabbits were used in this study, divided randomly into 4 groups (6 rabbits per group), induction of corneal ulcers centrally in groups (I, II) and peripherally in (III, IV). Treated groups are (I, III) using topical lyophilized canine Mesenchymal Stem Cells derived EVs & control groups are (II, IV) using topical normal saline. Result:...

Hussein Jabar Jasim1*, Amer Murhum Al-Amery2

...wed hyperplasia, fibrous proliferation in the portal area and coagulative necrosis of hepatocytes with large numbers of inflammatory cell infiltration such as eosinophil, Kupffer cell and scattered lymphocytes in the area previously migrated by young flukes as well hepatocytes displayed a fatty change with dilated hepatic sinusoids. Furthermore, our findings show that the bile ducts have hyperplasia, which is associated with fibrous cholangitis and an abundanc...

Jie Chen, Qinghua Qiu, Yanjiao Li, Xianghui Zhao, Lanjiao Xu, Xiaowen Xiong, Qingqi Wen, Mingren Qu and Kehui Ouyang*

...cutaneous pre-adipocytes proliferation rate, differentiation degree and lipid accumulation were higher than that of visceral pre-adipocytes both in HGM and LGM. In HGM group, autophagy marker genes i.e., microtubule-associated protein 1A/1B-light chain 3 (LC-3) and lipolysis protein i.e., hormone-sensitive lipase (HSL) expression in visceral pre-adipocytes were higher than that in subcutaneous pre-adipocytes, but adipogenesis transcriptional factors such as st...

Dalia Zaafar1*, Heba M.A Khalil2, Soha Hassanin3, Mohamed R. Mousa4, Mona G. Khalil1 

...an either inhibit cancer proliferation or promote cancer cell apoptosis. Methods: This study examined the effectiveness of pioglitazone (Pio) against mice bearing Ehrlich solid carcinoma in terms of its ability to inhibit cell proliferation and angiogenesis. Six groups were created using sixty male mice after the Ehrlich inoculation tumor growth was confirmed: control, Cis (5 ml/kg), Pio low dose (LD) (17.5 mg/kg), Pio high ...

Yuhua Chen1 and Song Lu2*

...rence of tumors, promote proliferation and activation of T cells, and strengthen the immune clearance of tumor cells.

...

Yang Hu, Jingxin Ding, Beilei Xu, Xiangming Sun, Guoyu Li, Hui Song, Zheng Zong and Wenlan Li*

...uated by determining the proliferation rate of Michigan Cancer Foundation-7 cells. The spectrum-effect relationship was analyzed by serum fingerprint of TGs to characterize the activity in vivo and identify the active compounds of TGs. In the results, a total of 75 chemical compounds of TGs were identified in serum, including 11 parent molecules and 64 metabolites. The serum fingerprint of TGs were established, and 36 common peaks were identified. The correlat...

Muhammad Akram¹,²*, Rashid Mahmood³, Fahim Arshad4 and Umer Farooq Gohar5

...r shoot regeneration and proliferation in the growth regulator-free MS liquid medium (95.25%) as compared to the solid medium (89.00%). Shoot cultures remained viable for up to 15 days without refreshing the medium and could be sustained for 12 months when sub-cultured every 15 days. Invigoration of albino shoots with fresh MS basal medium resulted in multiple green buds, which successfully transformed into 54.60 and 652.78 healthy shoots in 30 and 120 days, r...

Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2

... effect that reduces the proliferation of cancer cells caused by the carcinogenic substance. 
 
Keywords | Anticancer, Biochemical indices, Histopathology, Herbal medicine, Saussurea costus root extract
...

Lu Xie1,2,3,4,5, Ye Gu1,2,3,4, Qiang Liu1,2,3,4, Hongzhang Shen1,2,3,4, Yifeng Zhou1,2,3,4, Jiangfeng Yang1,2,3,4, Xiaofeng Zhang1,2,3,4* and Jinyu Huang1,5*

...cellular carcinoma cells proliferation, invasion and migration via post‑translational regulation of E2F1. In this study, we comprehensively analyzed the functions and mechanisms of the SET7/9-E2F1 axis using data mining. Data from the UALCAN database showed abnormal expression of both SET7/9 and E2F1 in multiple cancer types. Survival curves and correlation analysis by GEPIA supported the significant roles of SET7/9 and E2F1 in the progression of HCC. Functi...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang* and Jinhua Chen and Miao Tian

...vely. We found that cell proliferation rates in CON, TGT and DGT groups showed that cells were transfected with trangelin gene, the cell proliferation rate died down, significantly lower than that in CON and DGT groups (p<0.05). Cells migration and invasion results in all groups showed that after cells were transfected with trangelin gene, the number of migrated cells decreased significantly lower than in CON and DGT grou...

Dajun Su1, Tao Deng2* and Mingshui Xie2

...LOXL1-AS1 regulating the proliferation and apoptosis of ovarian cancer cells by targeting miR-761. Ovarian cancer cells SKOV-3 and normal ovarian cells FTE187 were divided into groups according to the experimental procedures, and were transfected negative, respectively. The expressions of lncRNALOXL1-AS1 and miR-761 in ovarian cancer cells were measured by qRT-PCR (quantitative real-time PCR). LncRNALOXL1-AS1 targeting miR-761 was detected by dual luciferase r...

Yakun Ge

...ntensity detection. Cell proliferation was detected by MTT method. Combined treatment promoted the production of ROS. The cell viability of the combined treatment group was lower than that of the control group at 24h, 48hs and 72h (P<0.05). Compared with the control group, the expression level of E-cadherin mRNA in the combined treatment group increased (P<0.05), and the expression levels of N-cadherin and vimentin decreased (P<0.05). Compared with th...

Lili Zhao1, Fan Zhao1 and Yaping Jin2*

...nism of trophoblast cell proliferation, invasion and hormone secretion remains elusive. In this study, we explored the role of GRP78 in the functionalities of goat trophoblast cells (GTCs). The Grp78 gene was efficiently knockout by using the CRISPR/Cas9 system, which resulted in altered morphology and function of GTCs. The cell shape showed a subrounded configuration, and the cell size also significantly increased. Furthermore, Grp78 knockout significantly de...
Nabila Rasheed1, Shumaila Usman2*, Madeeha Sadiq3, Bushra Wasim3, Kanwal Haneef4, Hafsa Shafqat3, Tabinda Urooj3 and Saman Rashid4
 
...abetes mellitus (GDM) on proliferation of WJMSCs (GDM-WJMSCs). Mesenchymal stem cells were isolated from Wharton’s jelly tissue of normal(healthy) samples and from the GDM affected sample. The isolated cells were characterized for the presence of stem cells markers by Immunocytochemistry. After characterization, the analysis of GDM effect on WJMSCS proliferation rate was analyzed by calculating the population doubling ...

Fatima Ali1*, Rimsha Tahir1 and Tahir Rehman Samiullah2

...ing in rats by enhancing proliferation, mobilization and angiogenesis at wound site.

...

Mahmoud Abdelhady Metwally1*, Mona Abdelftah Ali1, Farouk Abdelmohdy1, Mohamed Kassab1, Tarek Kamal Abouzed2 and Khalil Fathy Abou-Easa1

...) assay showed decreased proliferation, while flowcytometry showed increased apoptosis and cell cycle arrest in the G0/G1 phase with inhibition of entry into the S phase. RT-PCR showed p53 upregulation and Bcl-2 downregulation in mRNA expression. Moreover, Western blotting and flowcytometry revealed elevated p53 and lowered Bcl-2 protein levels. In addition, ELISA findings showed decreased toll-like receptor 4 (TLR4) concentration. Taken together, our findings...

Di Zhou1, Qingmeng Long1*, Rong Yang1, Xiaoshan Tan1, Jun Li1, Mingyan Tang1, Zhonghai Zhao3, Ye Ao2, Zhinan Zhou1 and Changxue Chen1

...alytic activity and cell proliferation. Moreover, these DEGs were enriched in the categories of signal transduction, reproductive system, endocrine system and cancer-related pathways and linked the cGMP-PKG, cAMP, TGFβ and mTOR signaling pathways to reproductive performance. We verified these results using qRT-PCR for 9 up-regulated (AMH, CYP19A1, FOXL2, FSHR, GATA4, INHBA, LHB, LHX9 and ZP3) and 9 down-regulated (CNOT1. DMRTA1, GHRHR, Hox-All, Oviductin,...

Salma Jameel Askar 

...d increased Kupffer cell proliferation, enlarged and apoptotic hepatocytes. Liver sections of G3 showed less side effect than Meloxicam-treated group. From this study concluded supplementation with beetroots ethanolic extract may attenuate the toxicity of Meloxicam, leading us to deduce that Meloxicam induces hepatotoxicity and creates abnormalities in hematological and biochemical markers. 

...

Yanmei Wang1,2, Haoyun Li1, Lijuan Han1 and Wenkui Wang1*

...2F, thereby induced cell proliferation, caused pulmonary artery vascular remodeling, and lead to PH. This study aims to provide theoretical basis for finding new therapeutic targets for broiler PH.

...

Khamiss l , Omaima; El Helaly , Alexandra and Abol Ela 3, Said

...hat PEG improve the cell proliferation and decrease cell death .An infection with Sl NPV baculovirus (Nucleopolyhedrovirus) was carried out on (SL Omi) new established cells in the presence of PEG treatments which demonstrate that PEG has affected the penneability of cells to the NPV infection and enhances the viral replication.

...

Ahmed*, Amal A.; Khatab*, Eman A.H. ; Dawood*, Rehab A. and Ismeil*, Amira .M.

... kinetin ) were used for proliferation and micropropagation of the infected shoot clumps respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg/l NAA ) . Plantlets regenerated from meristem measuring 0.1 mm and 0.2 mm yielded (90 and 80 % ) for CLV and (92 and 80 ) for CarVMV virus-free tested plantlets respectively,while larger meristems (0.3 and 0.4 mm ) were not effective . As well as the plantlets survival was (20 , 35 , 65 and 80% ...

Eman, M. Abd El-Mottaleb.*; Ahmed, S.A."; Maysa, H.M.*; Salem, S.A.* and M. M. El-Shenawy

...terstitial pneumonia and proliferation of the cells lining the bronchioles. Severe edema and infiltration of mononuclear inflammatory cells with proliferative fibrous connective tissues were seen in the heart. Liver showed severe edema, fibrous connective tissues proliferation, various sized organized thrombus with severe infiltration by mononuclear inflammatory cells. Depletion of the white pulp of the spleen as well as ede...

Sahar, A. Youssef t ; M.M.A. Al-Dhaher 2 and A.A. Shalaby 

...AP) (1.5 mg/L) for shoot proliferation, and Indol butyric acid (IBA) (0.05 mg/L) for plants rooting. Before acclimatization, the plantlets were submitted to DAS-ELISA and RT-PCR in order to evaluate virus eradication. GFLV- and GLRaV-l-free plants (92.5 and 95 %, respectively) were obtained from the optimum size (I mm) of meristem tips (as indexed by DAS-ELISA). Of these, 85 and 87.5 % plants were found negative from GFLV and GLRaV-l, respectively, as indexed ...

S.Y.M. Mahmoud1 and M. Hashem2

...ed roots which developed proliferation of rootlets. The foliar symptoms appeared as yellowing rounded patches in the field, including general chlorosis, wilting and necrotic vein yellowing. Samples Of rhizomania-like symptom roots and its adjacent soil were taken from these areas and tested for the presence of BNYVV using indirect DAS-ELISA with specific polyclonal antibodies to C-terminal 60 amino acids of BNYVV coat protein and bait plants test, respectively...

Yan Wang1,2*, Lin Liu3, Na Li4, Yijun Zong1, Wei Liu1, Wenhua Xu1, Qi Wang1, Peijuan Zhang1 and Huiling Feng2,5*

...n aerobic glycolysis and proliferation of lung adenocarcinoma cells. Database analysis confirmed the association between LY6K expression and survival prognosis. Western blot was used to detect the protein expression of proteins. RT-PCR was used to detect the mRNA expression level of genes. Cell proliferation was discovered using the CCK8 test and plate cloning. The impact on the cell cycle was examined by using flow cytometr...

Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*

...ipheral blood lymphocyte proliferation response was evaluated by cell proliferation assay. The results showed that a target band of 583 bp was obtained by PCR in DNA vaccine PLGA microspheres group at 7th, 14th, 21st, 28th, 35th day post-injection. No 3-1E gene target band was found in the control group and the naked plasmid DNA vaccine group. It indicated that plasmid pcDNA3-1E existed in tissues of immunized chickens with ...

Li Min1, Yong Chen2, Shunying Wu3, Xuefei Zheng1 and Mucheng Xu1*

... that LPS can induce the proliferation of JDPSCs and ADPSCs, and the proliferation ability of JDPSCs is stronger than that of ADPSCs, and LPS could up regulate the expression of mineralization related proteins.

...

Sura Ayed Radam*, Inam Badr Falih

...uently used to stimulate proliferation of T-cells and activate the immune response. Aims The present study conducted for showing effect of Concanavalin-A, whole killed lyophilized Streptococcus pyogenes antigen(WKLA) on immune response of rat. Methods To achieve this purpose, 20 Albino male rats were divided equally into four groups and immunized subcutaneously(s/c), 1st group was immunized with (100 mg/ml) of S. pyogenes antigen with added equal volume of Fre...

Hong Chen1,2, Jiawei Liu1,2, Chuan Lin1,2, Hao Lv1,2, Jiyun Zhang1,2, Xiaodong Jia1,2, Qinghua Gao1,2 and Chunmei Han1,2*

...ment mainly promotes the proliferation and differentiation of antler chondrocytes, osteoblasts, and T lymphocytes through biological signals such as WNT, IHH, and IL2RA to ensure the development of antler tissue again after top pruning. To sum up, our data implied that BVES gene regulated mesenchymal stem cells’ proliferation and differentiation activities in response to ischemic stimuli to promote the healing and rapi...

Hafiz Ishtiaq Ahmad1, Jinlong Zhang1, Fuxun Luo1, Owais Iqbal2 and Yuying Wang1*

...number, root length, and proliferation rate as compared to the others. Therefore, seedlings exposed to the light combination of red and blue (RB) exhibited the highest chlorophyll a and b, total chlorophyll content, catalase and root activity. The soluble sugar, soluble protein, superoxide dismutase and peroxidase levels increased significantly under compound red, blue and green (RBG) light conditions, while malondialdehyde content was highly decreased in the ...

Zhiwen Xu

.../Slug promoted OSCC cell proliferation by regulating yes-associated protein 1 (YAP). In this cell culture study, an OSCC cell model was constructed, followed by transfection with Snail-inhibitor. The OSCC HSC-3 cells were treated, and their apoptosis and viability were evaluated. Finally, Western blot, flow cytometry assay, Transwell assay, and scratch assay were used to explore the molecular mechanisms by which Snail/Slug promoted OSCC cell

Tanveer Hussain1, Abdul Wajid1, Jabbar Khan2, Asif Nadeem1, Misbah Hussain1, Qurat ul-Ain1 and Masroor Babar1*

... plays a crucial role in proliferation of T cells. IL-2 triggers the release of pro and anti- inflammatory cytokines by activating several pathways. In present study, exon 1 of IL-2 gene of four local Pakistani breeds (Dera Din Panah, Beetal, Nachi and Kamori) was amplified by using reported ovine IL-2 primers. Amplified products of 4 breeds of goat were bidirectionally sequenced to decipher polymorphisms. Only a single substitution (T→A) was found in non...

Quratulain Amjad1,2 and Abdul Rauf Shakoori1,2*

...rix. Slit2 reduces tumor proliferation in lung cancer and glioblastoma cells whereas TGF-β is a well-described tumor inducer. The present study was aimed at deciphering the role of heparan sulphate in Slit2 mediated inhibition of cancer metastasis. Cancer proliferation was induced by TGF-β in lung cancer cells (H1650) and glioblastoma cells (SF767) and then the anti-proliferative role of Slit2 was analyzed in prese...

Wan Nurfarzana Wan Mohamad Zani1, Norrizah Jaafar Sidik1*, Asmah Awal2, Nurul Izzati Osman3, Lyena Watty Zuraine Ahmad1 and Mohd Khairi Nordin4

...onstrated superior shoot proliferation compared to semi-solid media, recording the highest mean shoot number of 3.5 ± 0.8 shoots per explant in media supplemented with 0.5 and 4.0 mg L-1 BAP. The longest shoots were observed in liquid media with 0.5 mg L-1 BAP, with an average length of 3.57 ± 0.44 cm. Subsequently, the explants underwent rooting in both semi-solid and liquid MS media, supplemented with various IBA concentrations over a 5-week pe...

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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