Advancement of KIF20B in the Process of Invasion and Metastasis of Colon Cancer Induced by EMT Through Gli1
Advancement of KIF20B in the Process of Invasion and Metastasis of Colon Cancer Induced by EMT Through Gli1
Wenfeng Lin1* and Qiqi Zheng2
ABSTRACT
The aim of this study was to determine the role of KIF20B in the metastasis of colon cancer cells. CCK-8 method and Transwell cell test were used to determine the proliferation, invasion, and migration of cells. Western blotting was used to determine the changes in expressions of vimentin ZO-1, E-cadherin, β-catenin, N-cadherin and Gli1. We found that at 48h~96h, compared with the control group, the proliferation ability of the group with overexpression of KIF20B was significantly increased (P<0.05), but decreased in the group with low expression of KIF20B (P<0.05). The invasion and migration ability of the group with overexpression of KIF20B were increased compared with the control group (P<0.05). Compared with the control group, the expression levels of Vimentin, N-cadherin and Gli1 in the group with overexpression of KIF20B were significantly increased, while the expression levels of ZO-1, E-cadherin, andβ-Catenin were decreased (P<0.05). It can be concluded that KIF20B can promote the EMT, proliferation, and metastasis of colon cancer cells in vitro.
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