Effect of TRAF6 on Proliferation of Prostate Transitional Zone and Peripheral Zone Cells
Effect of TRAF6 on Proliferation of Prostate Transitional Zone and Peripheral Zone Cells
Yulin Zeng
ABSTRACT
The objective of this study was to demonstrate the effect of ubiquitin ligase tumor necrosis factor receptor-associated factor 6 (TRAF6) on promoting proliferation (Pro) of prostate transitional zone (TZ) and peripheral zone (PZ) cells by activating the insulin-like growth factor 1-protein kinase/mammalian target of rapamycin (IGF1-Akt/mTOR) signaling, providing a research basis for benign prostatic hyperplasia (BPH) therapy. In vitro proliferative prostate tissue specimens from patients with BPH were collected and classified into PZ and TZ tissues. A TRAF6 interference lentivirus was utilized to measure the expression levels of IGF1 and TRAF6 in the prostatic tissue matrix. The impact of TRAF6 on cell growth in the matrix of TZ and PZ tissues was analyzed, the TRAF6 level in the matrix of TZ and PZ tissues was explored, and its role in the phosphorylation process under growth factor stimulation was demonstrated. IGF1 and TRAF6 levels in the matrix of TZ tissue were superior to those in PZ tissue. After TRAF6 expression was inhibited, the Pro activity of TZ matrix cells slowed down, while after it was enhanced, the Pro activity of PZ matrix cells was dramatically increased. The TRAF6 in the inhibition group of TZ tissue was greatly lower, while that in the enhancement group of PZ tissue was greatly higher. It was concluded that TRAF6 promotes Pro of prostate TZ and PZ cells by activating the IGF1-Akt/mTOR signaling.
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