Influence of Perilla Frutescens Essential Oil Added to Semen Extenders on Frozen-Thawed Canine Sperm Quality
Influence of Perilla Frutescens Essential Oil Added to Semen Extenders on Frozen-Thawed Canine Sperm Quality
Nguyen Van Vui*, Huynh Thanh Tan
ABSTRACT
Oxidative stress has a significant impact on canine sperm preservation, highlighting the importance of antioxidant supplementation to maintain sperm quality. Perilla frutescens essential oil, rich in bioactive compounds with strong antioxidant properties, can enhance sperm quality when added to semen extenders during storage. This study investigated the effects of Perilla frutescens essential oil (20, 25 and 30 µg/ml) in extenders on frozen-thawed sperm. The oil was extracted via steam distillation and sperm quality was assessed by motility, viability, membrane integrity and lipid peroxidation. The results indicated that concentrations below 25 µg/ml improved sperm quality, while 30 µg/ml had a negative impact. The 25 µg/ml extender showed the best sperm quality (P<0.05). In conclusion, Perilla frutescens essential oil positively affects sperm quality, with 25 µg/ml being the optimal concentration for reducing oxidative stress and enhancing preservation.
Keywords | Canine sperm, Frozen-thawed, Perilla frutescens, Essential oils, Antioxidants, Cryopreservation
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