Investigation of Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Activity in Some Inflammatory Conditions in Barki Sheep
Investigation of Matrix Metalloproteinase-2 and Matrix Metalloproteinase-9 Activity in Some Inflammatory Conditions in Barki Sheep
Asmaa A. Darwish
ABSTRACT
Inflammation is a double-edged weapon. Although it starts as a protective mechanism, it ends with serious cliniopathological alterations, which disappoint our therapeutic programs and frustrate our expectation for curing. This work aimed to assess the most important clinicopathological alterations related to sheep pneumonia, arthritis and enteritis and spot the light on MMP-2 and MMP-9 diagnostic and prognostic accuracy in these conditions. 80 barki lambs were divided into four groups control group (CG), pneumonic group (PG), arthritic group (AG) and enteric group (EG). Blood samples were collected from the four groups then haematologically, biochemically and statistically analyzed. A significant (P< 0.05) microcytic hypochromic anemia was observed in the PG and AG while, EG suffered from a macrocytic hyperchromic anemia. On contrast, a neutrophilic leukocytosis and lymphocytopenia was described in the three diseased groups. Biochemically, a significant (P< 0.05) increase in total protein, globulin, liver enzymatic activities, kidney function tests, total lipids, triglycerides, MMP-2 and MMP-9 concentrations was depicted in the three diseased groups. On the contrary, the total cholesterol, HDL-cholesterol, LDL-cholesterol, minerals, electrolytes, trace elements and total antioxidants capacity concentrations significantly (P< 0.05) decreased in the three diseased groups. Both of MMP-2 and MMP-9 yielded a sensitivity and NPV as 100% in the sheep pneumonia, arthritis and enteritis and correlated with some parameters in PG and EG. Ovine inflammatory conditions usually result in different clinicopathological changes. Monitoring of them may be useful in improving our therapeutic intervention and enhancing the animal response to treatment. Both of MMP-2 and MMP-9 is a good marker for sheep inflammatory conditions.
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