Isolation of ESBL E. coli From Slaughtered Chicken of Open Market in District Abbottabad
Isolation of ESBL E. coli From Slaughtered Chicken of Open Market in District Abbottabad
Mehwish Malik1*, Zanib Sadia2, Muhammad Sajid3, Hammidullah1, Yasir Amin1, Zubair Ali1 and Sohail Ahmad1
ABSTRACT
Introduction of third generation cephalosporin in 1980s has overcome the problem of antibiotic resistance and most of the diseases were treated properly at that time, but the current situation of excessive use of these antibiotics in the treatment plans of both human and veterinary medicine makes them resistant to many bacteria including ESBL E. coli. Resistance of microbes to antibiotics is becoming an alarming situation throughout the world and the infection caused by such bacteria is difficult to treat and handle and ultimately an economic challenge to Pakistan also. E. coli is worth important bacteria in poultry as it is an opportunistic cocco-bacilli, it may cause Colibacillosis, which is the major cause of mortality and morbidity in poultry population and ultimately of economic loss to the farmers and consumers and many other workers related to this industry. The aim of this study was to isolate the E. coli from slaughtered chicken of open poultry markets and to check that how much the Cephalosporins are resistance to ESBL E. coli in Abbottabad region. E. coli is isolated through General microbe’s culture methods and biochemical tests. The E. coli isolated is further tested for ESBL E. coli. The detection of ESBL E. coli is done through DDST and CDT. It was noted that out of 210 samples 08 samples were found negative (no growth of any bacteria found) and 20 samples were shown growth other than E. coli suggesting a total prevalence of E. coli as 86.6 %. The prevalence of ESBLs in cecum of broilers in the present study is 53% of the total samples tested which is 61% of total positive samples. This current situation of prevalence of ESBL E. coli warns poultry farmers to the wise use of medicine in their flocks and move towards the precautionary measures to compete the disease challenge.
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