MicroRNAs hsa-miR-222-3p, hsa-let-7b-5p, and hsa-let-7f-5p Regulate Their Putative Target Genes HMGA1 and CDKN1B in Pakistani Breast Cancer Patients
MicroRNAs hsa-miR-222-3p, hsa-let-7b-5p, and hsa-let-7f-5p Regulate Their Putative Target Genes HMGA1 and CDKN1B in Pakistani Breast Cancer Patients
Hafiz Muhammad Farooq Yaqub1, Ali Raza Awan1, Rashid Saif2,3, Sehrish Firyal1, Muhammad Tayyab1, Muti-ur-Rehman Khan4 and Muhammad Wasim1*
ABSTRACT
MicroRNAs are the class of noncoding small RNAs that have the capability to control gene expression by degrading mRNA or repressing the post-transcriptional process. In the current era, role of microRNAs is one of the promising diagnostic, prognostic and therapeutic tools for early detection of cancer, which normally play their role in the regulation of post-transcriptional gene expression via a mechanism of complimentary sequences and repression of target RNAs. This study focused on the expression pattern of three miRNAs, hsa-miR-222-3p, hsa-let-7b-5p, hsa-let-7f-5p and effect on two of their putative target genes HMGA1 and CDKN1B in human breast cancer samples. Qiagen miScript Primer Assay based expression analyses revealed the over-expression of all three miRNAs in most of the studied human breast cancer samples compared to normal breast tissue samples. Moreover, over-expression of hsa-222-3p, hsa-let-7b-5p and hsa-let-7f-5p, downregulated their target genes (HMGA1 and CDKN1B) in breast cancer samples compared to normal tissue samples.
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