Molecular Cloning and Expression of Taste Receptor Gene T2R1 of Obscure Puffer, Takifugu fasciatus
Yijin He1, Song Ma2, Bo Liu1,*, Ting Xue1, Qunlan Zhou1, Wu Jin1 and Kui Chen2,*
1Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, No. 9 Shanshui East Road, Wuxi 214081, China
2School of Life Sciences, Soochow University, Suzhou 215123, China
* Corresponding author: chenk@suda.edu.cn; liub@ffrc.cn
Fig 1
Amplification of taste receptor T2R1 gene of Takifugu fasciatus.
Fig 2
Relative expression of T2R1 in different tissues of Takifugu fasciatus, A, gill; B, spleen; C, heart; D, lips; E, skin; F, tongue.
Fig 3
The similarities of T2R1 amino acid sequences between T. fasciatus with other animals. Note: the amino acids are numbered along the left and right margin. The transmembrane domains are underlined.
Fig 4
Phylogenetic tree revealing the relationship of T2R1 in T. fasciatus relative to other T2Rs family members of other species. Note: the horizontal branch length is proportional to amino acid substitution rate per site. The numbers represent the frequencies (%) with the tree topology presented was replicated after 1000 iterations.
Fig 5
The structural domain of T. fasciatus T2R1 protein
Note: transmembrane region 1. 10-32bp; 2. 39- 61bp; 3. 81-103bp; 4. 124- 146bp; 5. 179- 201bp; 6. 222- 244bp; 7. 259- 278bp
Fig 6
Signal peptide analysis of T2R1 protein of T. fasciatus.
Fig 7
Transmembrane helices prediction of T2R1 by TMpred online tool.