Negative Association of HLA-DRB1*11 and HLA-DRB1*12 Alleles with Aeroallergy Patients Visiting Allergy Centre (NIH), Islamabad, Pakistan
Negative Association of HLA-DRB1*11 and HLA-DRB1*12 Alleles with Aeroallergy Patients Visiting Allergy Centre (NIH), Islamabad, Pakistan
Madiha Hashmi1, Abid Hussain1, Shafiq ur Rehman1, Farida Ahmed2, Shahbaz Aslam3,Nadeem Afzal4 and Zaigham Abbas1,*
HLA typing using PCR. M, DNA 1kb marker; C, positive controls for allele of interest; A, PCR with primers of internal control GAPDH (PCR product size = 576bp); B, PCR with primers of allele of interest; Allele of interest in a, HLA-DRB1*11 (PCR product size = 176bp); Allele of interest in b, HLA-DRB1*12 (PCR product size = 248bp); Lanes 1 to 17, Samples of aero-allergic patients.
Percentage prevalence of HLA-DRB1*11 (A) and HLA-DRB1*12 (B) amongst aero-allergy cases reported at Allergy Centre, NIH, Islamabad and healthy controls.