Phytochemical and Antimicrobial Potential of the Roots of Berberis brevissima Jafri. (Berberidaceae)
Phytochemical and Antimicrobial Potential of the Roots of Berberis brevissima Jafri. (Berberidaceae)
Zain Ullah1*, Anwar Ali Shad1 and Saqib Ali2
ABSTRACT
Chemical screening on ethyl acetate fraction (Fr. C) obtained from the roots of Berberis brevissima Jafri. resulted in three compounds. Their structures were elucidated as β-Sitosterol-3-O-β-ᴅ-glucopyranoside (1), Berberine (2), and Pakistanine (3) using spectroscopic analysis, including mass, 1D, and 2D NMR data techniques. The antibacterial assays of the obtained six oil sub-fractions (H-1 to H-6) were accomplished against S. epidermis, E. coli, and S. aureus bacterial strains and the results ranged from 10-18 mm zone of inhibition. The GC-MS analysis of the six oil sub-fractions (H-1 to H-6) confirmed the existence of linoleic acid, palmitic acid, and oleic acid in the highest amount.
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