Potential Antioxidant Compound From the Culture of Endophytic Fungi from Surian Trees (Toona sinensis Roem)
Potential Antioxidant Compound From the Culture of Endophytic Fungi from Surian Trees (Toona sinensis Roem)
Noor Rahmawati*, Dea Indriani Astuti and Pingkan Aditiwati
ABSTRACT
Due to genetic exchange between host plants and endophytes, endophytic fungi can produce the same secondary metabolites as their host plants. Numerous research on the bioactive elements of secondary metabolites from endophytic microorganisms, particularly endophytic fungi, have been undertaken on plants that are able to produce secondary metabolites with unique properties. When tested using the DPPH radical-scavenging assay, the secondary metabolite Toona sinensis Roem, which was extracted using 80% acetone, shown antioxidant activity. To create antioxidant chemicals, the endophytic fungus T. sinensis must be isolated and researched. Thin-layer chromatography (TLC) and spectrophotometric methods were used right away to assess the potential of endophytic fungus to create antioxidant chemicals. Additional investigation on the endophytic fungi that produce antioxidant chemicals was carried out using ITS molecular analysis. Thirty strains of endophytic fungus were taken from twigs and leaves. Based on the early TLC examination, a number of endophytic fungus showed the presence of compounds with antioxidant function. Endophytic fungi crude extracts have been shown to be able to inhibit DPPH radicals by up to 86 percent by A26 and A19 and by 80 percent by B3 in spectrophotometric studies on cultures with antioxidant activity. This is the first report on endophytic fungus isolates from branches and leaves of T. sinensis that had antioxidant potential. The fungi were identified as Giberrella sp. and Fusarium sp. Future research on these microbes is highly encouraged because the results of this study indicate that they may be a reliable source of bioactive compounds, notably antioxidants.
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