Prevalence and Antimicrobial Resistance of Staphylococcus aureus, Salmonella and Escherichia coli Isolated from Poultry Meat in Tandojam, Hyderabad, Pakistan
Prevalence and Antimicrobial Resistance of Staphylococcus aureus, Salmonella and Escherichia coli Isolated from Poultry Meat in Tandojam, Hyderabad, Pakistan
Samia Sharafat1, Dildar Hussain Kalhoro1*, Muhammad Saleem Kalhoro1, Shahid Hussain Abro1, Mazhar Hussain Mangi1, Azhar Ali Laghari2, Ali Raza Nizamani3, Abdul Ahad Soomro3, Rani Wagan1, Asmatullah Kaka1, Abdullah Channo1, Hubdar Ali Kolachi4, Muhammad Ibrahim Panhwar4 and Mehkar Hussain5
ABSTRACT
Poultry meat is mostly consumed by people due to its good quality of protein and presence of all essential amino acids. In the present study, 100 poultry meat samples were randomly collected from district Hyderabad and cultured on selective media for the isolation and identification; mannitol salt agar for Staphylococus aureus (Staph. aureus), Salmonella Shigella agar and brilliant green agar for Salmonella and MacConkey agar for Escherichia coli (E. coli.). Furthermore, biochemical tests were performed for the confirmation of bacterial species. Overall, 19%, 35% and 56% prevalence were found for Staph. aureus, Salmonella and E. coli, respectively. Antimicrobial resistance of fourteen antibiotics (gentamycin, erythromycin, penicillin, ampicillin, vancomycin, neomycin, tetracycline, oxytetracycline, doxycycline, bacitracin, cephalothin, norfloxacin, streptomycin and kanamycin) were performed by disk diffusion method against Staph. aureus, Salmonella and E. coli showed resistance against all drugs. However, Staph. aureus showed high resistance to norfloxacin 84%, penicillin and ampicillin 78%, tetracycline, oxytetracycline and kanamycin 73%, bacitracin, doxycycline and vancomycin 68%, cephalothin 63%, neomycin 36%, erythromycin and gentamycin 31% and streptomycin 15%. Salmonella showed high resistance to ampicillin 94%, penicillin 91%, oxytetracycline 88%, bacitracin and neomycin 85%, doxycycline and tetracycline 82%, erythromycin, gentamycin and cephalothin 80%, kanamycin 74%, vancomycin 68%, norfloxacin and streptomycin 62%. Whereas, E coli showed high resistance to penicillin and ampicillin 87%, norfloxacin 85%, tetracycline and doxycycline 82%, neomycin and streptomycin 80%, doxycycline 78%, vancomycin 76%, kanamycin 75% and gentamycin 8%. In conclusion, high prevalence of Staph. aureus, Salmonella and E. coli were observed in poultry meat. The bacteria were found to be highly resistant to penicillin and ampicillin. Proper use of antibiotics is recommended to control the emergence of drug resistance.
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