Prevalence of New Delhi Metallo Beta-Lactamase (NDM) Producing Gram-Negative Bacteria from Different Tertiary Care Hospitals in Lahore, Pakistan
Prevalence of New Delhi Metallo Beta-Lactamase (NDM) Producing Gram-Negative Bacteria from Different Tertiary Care Hospitals in Lahore, Pakistan
Farzana Rashid1, Rabia Masood1 and Mariam Faiz2*
Resistance pattern of MBL production E. Coli (A), K. pneumonia (B) and P. aeruginosa (C) strains against various antibiotics; R: Resistance; S: Sensitive; MBL: Metallo-β-lactamases; IPM: Imipenem; MEM: Meropenem; FEP: Cefepime; CIP: Ciprofloxacin; CAZ: Caftazidime; CTX: Cefotaxime; CRO: Ceftriaxone.
PCR amplification of blaNDM-1 gene in different bacterial strains. M: DNA ladder 100bp. Lane 1,3,6: Amplified PCR product of P. aeruginosa blaNDM-1 gene. lANE 2: Amplified PCR product of K. pneumoniae blaNDM-1 gene. Lane 8,9,10: Amplified PCR product of E. coli blaNDM-1 gene.