Protective Effect of Paeoniflorin on Hypoxia Reoxygenation Cardiomyocytes and its Mechanism Based on MAPK Signal Pathway
Shu-Zhi Qin, Wen-Pei Ling, Mei-Fang Yin, Chun-Yu Luo and Cheng-Guo Zhao*
College of Pharmacy and Food Science, Zhuhai College of Science and Technology, Zhuhai 519041, China
* Corresponding author: zcg@zcst.edu.cn
Fig. 1.
Effect of different PF concentrations on H9C2 cell viability (a) under normal oxygen culture and (b) with H/RI treatment.
Notes: x̄ ± s, n = 3; Compared with 0 μmol/L, * P <0.05; Compared with H/R group, # P <0.05.
Fig. 2.
Effect of PF on AST and LDH levels in H9C2 cells injured by H/R.
Notes: x̄ ± s, n = 3; ** P <0.01, compared with control group; ## P <0.01, compared with H/R group.
Fig. 3.
Effect of PF on apoptosis rate of H9C2 cells injured by H/R.
Notes: x̄ ± s, n = 3; ** P <0.01, compared with Control group; ## P <0.01, compared with H/R group.
Fig. 4.
Effect of PF on ROS level in H9C2 cells injured by H/R.
Notes: x̄ ± s, n=3; ** P <0.01, compared with Control group; ## P <0.01, compared with H/R group.
Fig. 5.
Representative picture of Caspase-3, Bcl-2, p38 MAPK, ERK1/2, and JNK in H9C2 cell of each group (a), and Effect of PF on the expression of Caspase-3 (b), Bcl-2 proteins (c), p38 MAPK (d), JNK (e), and ERK1/2 (f) proteins in H9C2 cells.
Notes: x̄ ± s, n=3; * P <0.05, ** P <0.01, compared with control group; # P <0.05, ## P <0.01, compared with H/R group.