Study on Populations of Cabbage Cyst Nematode Heterodera cruciferae Franklin, 1945 from Iran
Study on Populations of Cabbage Cyst Nematode Heterodera cruciferae Franklin, 1945 from Iran
Habibeh Jabbari
ABSTRACT
Cabbage cyst nematode, Heterodera cruciferae Franklin, 1945 causing serious damage on different cabbage cultivars and other host plants. High incidence of this nematode revealed by soil and root samples in the vegetable growing farmlands in Tabriz, Iran. Different populations of H. cruciferae were collected from the region and characterized based on morphological, morphometric and molecular features, four out of those populations isolated from the rhizosphere and roots of four different cabbage cultivars were subjected to further studies. All the populations showed same range of morphometric and morphological characters fitted thoroughly with the identity of H. cruciferae. Different primers for amplification of ITS1-5.8S-ITS2, D2-D3 rDNA and hsp 90 genes, showed similarity between the nematode populations. ITS-RFLP profiles generated by 16 different restriction enzymes did not differentiate the populations, too. In phylogenetic analyses of partial sequences of 28S rDNA D2-D3, a clade clustering the four populations of cabbage cyst nematode was formed along with the sequences of H. cruciferae populations deposited in NCBI. These results showed morphometrical, morphological and molecular characters of under study populations are same and the host is the only differences between pathogenicity populations.
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