Sugar Press Mud as a Proposed Futuristic Aqua-fertilizer - Multi-Elemental Quantitative Characterization by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)

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PJZ_50_4_1329-1340

Sugar Press Mud as a Proposed Futuristic Aqua-fertilizer – Multi-Elemental Quantitative Characterization by Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES)

Kashifa Naghma Waheed1,2,*, Zaid Mehmood2 and Sikender Hayat1

1Fisheries Research and Training Institute, Department of Fisheries, Lahore

2Institute of Chemistry, University of the Punjab, Lahore

ABSTRACT

In this research, characterization of press mud, a by-product of sugar industry in respect of its multi-elemental availability was taken into consideration to check its feasibility as an aqua-fertilizer. Eighteen elements including essential macro-elements, essential trace elements and non-essential toxic elements were quantitatively analyzed in sugar press mud by ICP-OES following protocols of AOAC from five randomly selected sugar mills situated in South Punjab, Pakistan. It was observed that the samples contained potential amounts of essential elements, while the toxic elements were present in low quantities. The results were statistically analyzed and compared for their significance and showed significantly higher quantities of Ca, Na, K, Fe, Mg followed by relatively lower quantities of Al, Mn and low quantities of Zn, Co, Ba, Li, Pb, Cu, Sr, Ni, Cr, La and Cd. It was concluded that this naturally fortified by-product of sugar industry can be beneficially employed as a valuable futuristic fertilizer in aquaculture processes.

Article Information

Received 02 August 2016

Revised 13 January 2018

Accepted 28 March 2018

Available online 25 May 2018

Authors’ Contribution

KNW conceived the project, collected the samples, planned and performed the research, statistically analyzed the data and composed the research paper.

ZM and SH guided, facilitated and supervised the research.

Key words

Essential macro-elements, Essential trace elements, ICP-OES, Non-essential toxic elements, Sugar press mud.

DOI: http://dx.doi.org/10.17582/journal.pjz/2018.50.4.1329.1340

* Corresponding author: kashifanw@gmail.com

0030-9923/2018/0004-1329 $ 9.00/0

Introduction

Pakistan is the 8th largest sugar producer country in the world, at 6th position in terms of sugarcane production and at 7th position in terms of export of white sugar among the Global sugar production countries on the basis of evaluation conducted in 2014 by Pakistan Sugar Mills Association. The total area under sugarcane cultivation comprises of 1.172 hectares/tonnes producing 67.428 hectares/tonnes of sugarcane. The sugar production remained at 5.588 hectares/tonnes out of Sugarcane crushed @ 56.460 hectares/tones and the cane utilization remained at 84%. In present scenario, the Sugar Industry is the second largest agro-based industry in Pakistan following Textile Industry. There are a total of 82 Sugar Mills in this country in the vicinity of sugarcane cultivation areas; out of which 45 (44 in working conditions) are situated in the province of Punjab covering 55% of the total Mills and 60% of the total cane sugar production of the country. The South Punjab holds 13 number of Sugar Mills among a total of 44 (PSMA, 2014).

During filtration of sugarcane juice, certain by- products including bagasse, press mud and molasses are also released. Out of these, Sugar press mud also known under the names of filter cake and mud cake is an important but neglected by-product which is produced @ 3-4% of the total fresh sugarcane input on the annual basis. This compressed by-product obtained from sugar mills during the sugar manufacturing process, is reported to be a soft, spongy, amorphous, dark brownish material containing almost 75-80% moisture, high organic carbon contents along with appreciable amounts of macronutrients (N, P, K, Ca, Mg, S) and micronutrients / trace elements, also (Kale and Shinde, 1986; Solaimalal et al., 2001; Rashi et al., 2005). There is still no market of press mud in Pakistan; it is merely wasted resulting in heaps and heaps of this agro-industrial waste being piled up in the grounds adjacent outside the Mills. United Nations Industrial Development Organization (UNIDO) also reported that nearly 1.2 million tons of press mud produced during 2009 in Pakistan was mostly discarded by the sugar industries (Naseem, 2009). However, this by-product can be utilized primarily not only as a source of energy but also as a source of nutrients, soil ameliorants and fertilizer, too. Its re-use can be very useful to the farming and milling sectors as well as supporting other industries (Qureshi et al., 2001). It is indeed mutually beneficial to the horticulture and agricultural crops from point of view of its richness in various micronutrients (Partha and Krishnan, 2000; Saravane et al., 2005; Partha and Sivasubramanian, 2006).

There are reports of use of press mud as fuel/energy source in brick production Kilns in Pakistan but never utilized as a fertilizer in freshwater aquaculture/fish culture systems. This preliminary study has been taken up for the first time to investigate the Multi-Elemental analysis including essential and non-essential metal profile determination of this neglected valuable by-product of sugar industry to check its feasibility as a futuristic fertilizer in fish culture system since there is very less and limited information regarding the amount of elements present in sugar press mud from all over the world. This will be a great contribution and support towards the economic strengthening of the region by using this waste for reducing, recycling and reusing purposes through 3R’s formula.

Materials and methods

Study area, sampling stations and sample collection

The Sugar press mud (SPM) samples were fetched from randomly selected five Sugar Mills namely The Thal Industries (unit 1) Corporation Ltd. (Layyah), Indus Sugar Mills Ltd. (Rajanpur), Fatimah Sugar Mil (Muzaffargarh), Etihad Sugar Mills Ltd. (Karamabad, Rahim Yar Khan) and Ashraf Sugar Industries (Ashrafabad, Bahawalpur). The samples were collected in large sterilized polythene bags directly from the outlets pipes, coming from press mud wheelers inside the premises of Mills, in order to avoid any contamination and then were properly labeled. The safety hazards associated with the samples collection were taken into consideration. These samples were then transported to the FR&TI, Lahore. Each individual sample was mixed thoroughly, placed in separate labeled trays and allowed to air dried completely. The representative sub-samples (of each Sugar Mill) were then randomly collected in triplicate (03 in number) from the air dried bulk of each particular Mill; homogenized by pastle mortar and preserved in small tightly sealed properly labeled polythene bags till the initiation of digestion for way up to multi-elemental analysis of theses samples.

Machinery and equipment

The machinery and equipment used in this research included Inductively coupled plasma-optical emission spectrometer (Brand: PerkinElmer, Model: Optima 7000 DV, Made: USA) with all accessories, environment friendly ductless Fuming hood, digital heavy duty Hot plate with controllable variable temperature and capacity 1000oC (Made: PCSIR, Lahore), Utrapurification system (Brand: Millipore). All laboratory formalities were considered and precautionary measures needed to be taken into account were implemented during the entire experimental work like sampling technique, acid digestion processes, acid fumes/toxics handling, gas cylinders hazards and plasma precautions, etc. The Instrumental operating conditions applied during the entire analysis are evident from Supplementary Table I and the ICP-OES Laboratory environmental conditions maintained during the present research were as follows: Temperature, 18±2°C; relative humidity, 45±5%; cleanliness, dust free; location, vibration free; atmosphere, fumes free. The limits of detection of the method and instruments were developed and optimized as expressed in Supplementary Table II. The elemental parameters including applied selected wavelengths (nm), working standards range, correction coefficient of calibration (obtained through calibration curves of standards) and resulting quality control checks (% recovery) through spiking can be visualized from Supplementary Table III.

Apparatus/glassware

The glassware used for the preparation of chemicals, reagents, stock solutions, working standards and sugar press mud (SPM) samples comprised of volumetric flasks, beakers and pipettes got calibrated from PCSIR, Internationally Accreditated Laboratories, Lahore, Pakistan.

Reagents

All chemicals/reagents used were of Analytical reagent grade, Merck brand and required strengths prepared with double de-ionized (DDI) ultrapure water obtained from Millipore ultra-purification system. The working standard solutions were prepared from the provided strengths combinations as shown in Supplementary Table III by further and serial dilutions as required by the method within the ranges observed for the detection of the required elements.

Technical section reference

The SPM samples digestion and then selected metals determination therein were obtained following AOAC official methods of analysis, 990.08 for Metals in solid waste, Section 9.2.39.

Analytical procedure for wet sample digestion

The analytical procedure adopted for digestion of all SPM samples is briefly described as under. Initially, 2.0±0.005 g of each of preserved dried homogenized press mud sample was weighed, transferred to a 100 mL beaker and then 15 mL conc. HNO3 was added to it. The beaker was covered with a watch glass and heated at 95°C for 30 min. The precaution was taken to prevent it from drying by putting 1-2 mL DDI water as and when required. Afterwards, 2 mL water + 10 mL 30% H2O2 were added to it and heated slowly till the solution became transparent. This was cooled the final volume made upto 100 mL with DDI ultrapure water, filtered through 0.45 µm filter paper for removal of any particulate material, preserved in the refrigerator at 4°C in 125 mL properly labelled tightly stoppered glass storage bottles.

ICP-OES determination

During the multi-elemental analysis in Sugar Press Mud samples by ICP-OES instrument, the quality assurance was taken into consideration through initializing quality control checks (QCS) i.e., preparing spike samples by spiking the SPM sample with known amount of element and then finding its % recovery after analyzing on the same instrument during analysis of all other samples for the same metal. These results have been recorded in Supplementary Table III. The SPM samples were prepared and analyzed in triplicate for all the metals, moreover, each metal was also subjected to instrumental triplicate element analysis for which no variation in results were observed confirming the accuracy of the sample preparation, accurate calibration procedures and validity of applied method. The WinLab32 software operating system under Microsoft Windows operating systems was used as a tool.

The following relationship was used for calculating final concentration of the elements under consideration:

FCE = (EC - B) × DF

Where, EC is element concentration obtained from the ICP-OES instrument, B is concentration of the laboratory reagent blank (LRB), DF is dilution factor (equal to 50 since 2 g was diluted to 100 mL) and FCE is final concentration of element in the SPM sample obtained from calculating all the above

Statistical analysis

The reported results obtained through research were subjected to statistical analysis following Steel et al. 1996. The results description involved following five steps. 1) Bar comparison graphs of mean ± SEM values of triplicate sample analysis having standard error bars for all elements of five sites (on Microsoft Excel), 2) Descriptive statistics including minimum, maximum, median, arithmetic means, standard error, standard deviations, variance and 95% confidence interval values (SPSS 21 package programme), 3) One way Analysis of Variance (ANOVA) to find out the level of significance (SPSS 21 package programme), 4) Correlation analysis considered significant at P < 0.05, P < 0.01, P < 0.001 (SPSS 21 package programme) and 5) Summary of elements concentration in press mud from different locations of the world (literature survey).

RESULTS

The individual test results of multi-elemental concentrations (µgg-1 ± SEM dry weight) found in Press Mud samples of various Sugar Mills located in South Punjab, Pakistan are shown in Figures 1, 2 and 3. The results showed that all the eighteen elements under consideration were mostly and positively found in all the samples with the exception of the few non-essential metals. Among all the studied essential elements, Ca had the overall highest concentration in all the press mud samples collected from the five Sugar Mills followed by the rest of the elements i.e. Na, K, Fe, Mg, Al, Mn, Zn, Pb, Sr, Ba, Cu, Co, Ni, Li, Cr, La and Cd in descending order, respectively. The results of compiled descriptive statistics of above mentioned elements; including the minimum, maximum, mean, median, standard error, standard deviation, variance and 95% confidence interval of all the samples are presented in Supplementary Table IV. These metals were basically divided into three broad categories for ease in evaluation of the results i.e., essential macro-elements (including Ca, K, Mg, Na), essential trace elements (including Co, Cr, Cu, Fe, Mn, Ni, Zn) and non-essential toxic elements (including Al, Ba, Cd, La, Li, Pb, Sr). Our study showed that the contents of afore-mentioned three categories were different with respect to each other.

Essential macro-elements

The elements, Ca, K, Mg, Na required in large amounts, have essential and versatile role in fish growth, metabolic requirements and biological functions. The comparison of average/mean concentration with standard error bars of these essential macro-elements in SPM samples can be better understood from Figure 1. It is revealed that the Ca mean concentration (18005.00 ± 430.47 µg g-1) was found highest in Press Mud obtained from The Thal Industries (Unit-I) Corporation Ltd., Layyah while lowest (8040.00 ± 195.13 µg g-1) was observed in Indus Sugar Mills Ltd., Rajanpur in comparison with all the other Sugar Mills. Since Calcium is a key component in the maintenance of cell structure and fulfils important physiological roles as cofactor for many enzymes, important component of the blood clotting mechanism, an active role of intracellular signal, etc. Its deficiency leads to stunted growth, poor quality bones/teeth and bone malformation (Bucher et al., 1996; COMA, 1998).

The mean concentration of K remained at highest (6796.33 ± 53.36 µg g-1) in Press Mud of Fatima Sugar Mill, Muzafargarh while lowest (4266.93 ± 8.7 µg g-1) was observed in Ashraf Sugar Industries Ltd., Bahawalpur compared with all others. Potassium, together with sodium, is essential for the maintenance of normal osmotic pressure within cells, cofactor for numerous enzymes, required for secretion of insulin by the pancreas, for phosphorylation of creatine, for carbohydrate metabolism and for protein synthesis, also. Its loss results in Hypokalaemia, diarrhoea, diabetic acidosis, vomiting, intense and prolonged sweating (COMA, 1991; Fotherby and Potter, 1992; Grimm et al., 1988).

Sodium is also an essential mineral for regulating body fluid balance. Its deficiency is highly unusual, but can lead to low blood pressure, dehydration and muscle cramps (COMA, 1994; Ganry et al., 1993). The highest mean concentration of Na (8379.17 ± 108.70 µg g-1) was found in Press Mud obtained from Ashraf Sugar Industries Ltd., Bahawalpur while lowest (5885.17 ± 60.54 µg g-1) was observed in The Thal Industries (Unit-I) Corporation Ltd., Layyah.

The mean concentration of Mg (5077.83 ± 64.87 µg g-1) remained at highest in Press Mud of Ashraf Sugar Industries Ltd., Bahawalpur while lowest (4048.87 ± 31.66 µg g-1) was observed at Etihad Sugar Mills Ltd., Rahim Yar Khan from all others. Magnesium is the eighth most abundant element in the earth’s crust and is required as a cofactor for many enzyme systems, in protein synthesis, normal functioning of the parathyroid gland for vitamin D metabolism, etc. Its deficiency results in cardiovascular, skeletal, astro-intestinal and central nervous system disorders and to the use of loop diuretics (COMA, 1991; OTC, 2001; Zemel et al., 1990; Altura et al., 1994). The overall scenario showed that all the essential macro-elements were potentially present in high concentrations in all the samples collected from five different sites ranging from Mg having a minimum concentration i.e., 4048.87 µg g-1 to Ca having a maximum concentration i.e., 18005.00 µg g-1. The mean values of essential macro-elements remained predominantly higher at the Bahawalpur and Layyah regions.

The data obtained for individual elements was also compared statistically by one way ANOVA and the results are depicted in Supplementary Table V indicating F and P values. The results show highly significant P values (P < 0.0001) among all the elements. The correlation analysis was performed to examine the relationship between all the elements and the results are narrated in Supplementary Table VI. The essential macro-elements are interrelated with each other and with all other elements under consideration as described herewith. The Ca concentration had a strong positive significant relationship (P < 0.01) with Co, Fe and Ba concentration while it had a strong negative significant relationship (P < 0.01) with Na, Ni and Pb. It had a positive significant relationship (P < 0.05) with Cr and La. It had a non-significant relationship (P > 0.05) with K, Mg, Cu, Mn, Zn, Al, Cd, Li and Sr. The K concentration had a strong positive significant relationship (P < 0.01) with Cr, Cd, La, Li and Zn concentration while it had a strong negative significant relationship (P < 0.01) with Na and Ni. It had a positive significant relationship (P < 0.05) with Fe and Sr. It had a non-significant relationship (P > 0.05) with Mg, Co, Cu, Mn, Al, Ba and Pb. The Na concentration had a strong positive significant relationship (P < 0.01) with Ni concentration while it had a strong negative significant relationship (P < 0.01) with Cr, Fe, Zn, La and Li. It had a negative significant relationship (P < 0.05) with Co, Ba and Cd. It had a non-significant relationship (P > 0.05) with Mg, Cu, Mn, Al, Pb and Sr. The Mg concentration had a strong positive significant relationship (P < 0.01) with Fe, Mn and Ba concentration. It had a negative significant relationship (P < 0.05) with Ni. It had a non-significant relationship (P > 0.05) with Co, Cr, Cu, Zn, Al, Cd, La, Li, Pb and Sr. Our results exhibited that the contents of essential macro-elements present in press mud from all five sites can promise the requirements of an aquaculture fertilizer.

Essential trace elements

The elements such as Co, Cr, Cu, Fe, Mn, Ni and Zn are mainly needed in trace amounts for fish and human body functions. Thus, it can be appropriate to observe a comparative account of average/mean concentration with standard error bars of above mentioned elements from Figure 2. The maximum mean concentrations (µg g-1) of Co, Cr, Cu, Fe, Mn, Ni, Zn were observed at 34.12 ± 0.90 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 8.67 ± 0.26 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 49.39 ± 0.57 (Fatima Sugar Mill, Muzafargarh), 6671.83 ± 27.17 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 273.33 ± 6.58 (Ashraf Sugar Industries Ltd., Bahawalpur), 34.58 ± 0.50 (Etihad Sugar Mills Ltd., Rahim Yar Khan) and 122.67 ± 3.35 (Fatima Sugar Mill, Muzafargarh) while the minimum mean concentrations (µg g-1) were found as 19.66 ± 0.44 (Fatima Sugar Mill, Muzafargarh), 0.013 ± 0.003 (Etihad Sugar Mills Ltd., Rahim Yar Khan), 12.84 ± 0.36 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 2491.93 ±8.32 (Etihad Sugar Mills Ltd., Rahim Yar Khan), 109.28 ± 0.47 (Etihad Sugar Mills Ltd., Rahim Yar Khan), 8.10 ± 0.23 (The Thal Industries (Unit-I) Corporation Ltd., Layyah) and 85.16 ±1.36 (Etihad Sugar Mills Ltd., Rahim Yar Khan).

Cobalt being an integral part of vitamin B12 is an essential nutrient having important role in body metabolism (Blakhima, 1970) while Chromium is a trace element having essential role in biological functions i.e., diabetes mellitus control and glucose tolerance (Bratakos et al., 2002) but at the same time is also considered as a pollutant and a toxic metal due to its deployment in manufacturing industries. Copper is another essential micronutrient required for effective homeostatic control, body growth, bone strength, enzymatic functions, defense mechanisms, brain development, iron transport, oxygen transport, cholesterol and glucose metabolism, etc. (Turnlund et al., 1989; Olivares et al., 1998). Its negative balance may result in iron-deficiency/anemia (COMA, 1991; Frykman et al., 1994). Manganese, an essential trace element, is a component of a number of enzymes and many are activated by it. Its deficiency is associated with skeletal malformations, impaired growth and reproductive function (COMA, 1998; Kondakis et al., 1989). Nickel is also an essential element required in trace amounts basically for activation of some enzymatic functions; however, its chronic intake can lead to high risk of lung cancer (EGVM, 2003). Zinc is an essential constituent of more than two hundred metalloenzymes and plays a key role in the synthesis/stabilization of genetic material, synthesis/degradation of carbohydrates, lipids and proteins. Its deficiency results in effects including poor prenatal development, growth/mental retardation, impaired nerve conduction/nerve damage, reproductive failure, dermatitis, hair loss, diarrhea, loss of appetite, loss of taste and smell, anemia, susceptibility to infections, delayed wound healing and macular degeneration (COMA, 1991; Yadrick et al., 1989). The results showed that all the essential trace elements were present in moderate concentrations in all the samples collected from five different sites with the exception of Fe whose value was relatively high as compared to all other elements. The mean values of the essential trace elements remained predominantly higher at the Layyah and Muzafargarh regions.

The essential trace-elements are interrelated with each other and with all other elements under consideration as described herewith. The Co concentration had a strong positive significant relationship (P < 0.01) with Ba, while it had a strong negative significant relationship (P < 0.01) with Cu. It had a positive significant relationship (P < 0.05) with Fe, while it had a negative significant relationship (P < 0.05) with Ni. It had a non-significant relationship (P > 0.05) with Cr, Mn, Zn, Al, Cd, La, Li, Pb and Sr. The Cr concentration had a strong positive significant relationship (P < 0.01) with Fe, Zn, Ba, Cd, La and Li concentration, while it had a strong negative significant relationship (P < 0.01) with Ni. It had a non-significant relationship (P > 0.05) with Cu, Mn Al, Pb and Sr. The Cu concentration had a non-significant relationship (P > 0.05) with Fe, Mn, Ni, Zn, Al, Ba, Cd, La, Li, Pb and Sr. The Fe concentration had a strong positive significant relationship (P < 0.01) with Zn, Ba and Li, while it had a strong

negative significant relationship (P < 0.01) with Ni and Pb. It had a non-significant relationship (P > 0.05) with Mn, Al, Cd, La and Sr. Mn concentration had a strong positive significant relationship (p<0.01) with Al. It had a negative significant relationship (P < 0.05) with La. It had a non-significant relationship (P > 0.05) with Ni, Zn, Ba, Cd, Li, Pb and Sr. The Ni concentration had a strong negative significant relationship (P < 0.01) with Zn, Ba, La and Li. It had a positive significant relationship (P < 0.05) with Pb. It had a non-significant relationship (P > 0.05) with Al, Cd and Sr. The results indicated that the essential trace-elements available in press mud from all five sites can also fulfill the requirements of an aquaculture fertilizer.

Non-essential/toxic elements

The elements such as Al, Ba, Cd, La, Li, Pb and Sr are categorized as non-essential/toxic elements since these have no biological/metabolic functional roles to play in living organisms; rather these are harmful when ingested even in low quantities (Aziz et al., 2011). The comparison of average/mean concentration with standard error bars of these elements present in press mud samples can be effectively perceived from Figure 3. The maximum mean concentrations (µg g-1) of Al, Ba, Cd, La, Li, Pb, Sr were observed at 421.5833 ± 11.00 (Etihad Sugar Mills Ltd., Rahim Yar Khan), 53.9167 ± 0.83 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 1.7967 ± 0.03 (Indus Sugar Mills Ltd., Rajanpur), 5.1333 ± 0.04 (The Thal Industries (Unit-I) Corporation Ltd., Layyah), 27.2167 ± 0.81 (Fatima Sugar Mill, Muzafargarh), 73.1667 ± 1.15 (Indus Sugar Mills Ltd., Rajanpur), 67.4967 ± 0.99 (Indus Sugar Mills Ltd., Rajanpur) while the minimum mean concentrations (µg g-1) were found as 190.63 ± 5.07 (Indus Sugar Mills Ltd., Rajanpur), 28.65 ± 0.66 (Etihad Sugar Mills Ltd., Rahim Yar Khan), N.D. (Etihad Sugar Mills Ltd., Rahim Yar Khan and Ashraf Sugar Industries Ltd., Bahawalpur), N.D. (Ashraf Sugar Industries Ltd., Bahawalpur), 12.97 ± 0.09 (Ashraf Sugar Industries Ltd., Bahawalpur), 9.20 ± 0.22 (The Thal Industries (Unit-I) Corporation Ltd., Layyah) and 35.60 ± 0.98 (Etihad Sugar Mills Ltd., Rahim Yar Khan). The results indicated that all the non-essential/toxic elements were either not detected in some of the samples and even if present were in very low concentrations. This was a very positive sign so that it can be used as fertilizer without fear of toxicity and contamination of the aquatic media of Aquaculture systems. The mean values of non-essential/toxic elements remained predominantly higher at the Rajanpur and Layyah regions.

The non-essential toxic elements are interrelated with each other and with all other elements under consideration as described herewith. The Al concentration had a strong negative significant relationship (P < 0.01) with Cd and Sr, while had a non-significant relationship (P > 0.05) with Ba La, Li and Pb. The Ba concentration had a non-significant relationship (P > 0.05) with Cd, La, Li, Pb and Sr. Cd concentration had a strong positive significant relationship (p<0.01) with Sr concentration and a positive significant relationship (P < 0.05) with Li and Pb, while had a non-significant relationship (P > 0.05) with La. The La concentration had a strong positive significant relationship (P < 0.01) with Li and a non-significant relationship (P > 0.05) with Pb and Sr. The Li concentration had a non-significant relationship (P > 0.05) with Pb and Sr. The Pb concentration had a strong positive significant relationship (P < 0.01) with Sr concentration. The overall results demonstrated that the total non-essential elements present in press mud from all five sites were in low quantities making it possible to use it as an aquaculture fertilizer.

Discussion

Our research results are mostly in agreement with the previous published studies from various regions of the world as described in Table I. Memon et al. (2012) tested the potential of three agricultural waste composts in pot experiment growing maize and recommended that press mud could be recycled thus sustaining the balance between economic development and environmental protection. In another experiment by Cifuentes et al. (2013), press mud composite along with a mixture of bagasse substituted with waste sugarcane was used as a fertilizer on large scale in the greenhouse environment resulting in the maximum tomato plant height and weight. Moreover, Maheera et al. (2013) assessed that the soil amended with press mud attained the ability of reducing the concentration of heavy metals leachability and its application as a landfill soil cover can aid in landfill management to minimize heavy metals contamination and operating costs. Paksoy et al. (2018) evaluated the essential and heavy metals in milks of dairy donkeys, goats and sheep in Turkey in the same manner as under this research. Our study is also supported by Kumar et al. (2011) who analyzed press mud compost for zinc, manganese, iron and aluminum and concluded it to be a good source of soil elements and organic matter for application to the agricultural land. Furthermore, Rouf et al. (2010) collected press mud from sugar mills situated in Punjab, India and analyzed macronutrients and micronutrients on % dry basis and reported that the digested slurry of press mud could be used as a fertilizer since it was rich in plant nutrients. Ramesh et al. (2004) emphasized and proved that a judicious combination of inorganic, organic and biofertilizers is a potential tool for sustaining the cane productivity as well as’ soil fertility in sugarcane and sugarcane based cropping systems.

Table I.- Summary of multi-elements found in press mud from different locations in the world.

Purpose of study	Element concentration (%) in sugar press mud	Element concentration (µgg-1) in sugar press mud	Location	Source
Mill mud case study	N (0.37%), P (0.227%), K (0.095%), Ca (0.585%) and Mg (0.157%)	S (0.0067)	Sugar Mill, Queensland, Australia	Barry et al. (1998) Chapman (1996)
For improving the physico-chemical properties of calcareous soil	-	N (20000), P (13000), K (19500), Ca++ Mg++ (5442), Na (750), Cu (64), Fe (322), Mn (298) and Zn (125)	Chashma Sugar Mills (Pvt) Ltd., D. I. Khan, KPK, Pakistan	Ghulam et al. (2010)
As Fertilizer on Agricultural land	N (0.85%), P (0.53%) and K (1.72%)	-	Sugar Mill, India	Kumar et al. (2010)
Press mud vermicompost preparation	Available P (1.80%), N (0.271%), Ca (0.204%), Mg (0.088%), Na (0.005%) and K (0.0058%)	-	Bidvi Sugar Mill, Sharanpur, U.P, India	Joshi and Sharma (2010)
As fertilizer on agricultural land	-	Na (0.22), K (0.40), Ca (2.11), Mg (0.33), Fe (0.32), Co (Trace), Mn (0.04) and Zn (0.01)	Sugar Mill, Punjab, India	Rouf et al. (2010)
As fertilizer on sugarcane crop	N (2.30 %), P (1.35 %) and K (0.75 %)	-	Sugar Factory, Faisalabad, Pakistan	Sarwar et al. (2010)
As organic fertilizer for agricultural soils	-	N, P (290), K, Zn, Cu, Fe and Mn	-	Khan (2011)
As organic matter application to the agricultural land	-	Fe (115), Al (288), Mn (6169) and Zn (2233)	Sugar Industry, India	Kumar et al. (2011)
Effects on water holding capacity of soil	N (2.67%), P (1.38%), K (0.6%), Ca (2.6%), Mg (1.3%) and Na (0.12%)	-	Sugar Mill, Kumbhi Kasari S.S.K, Kuditre from Kolhapur district, Maharashtra. India.	Bhosale et. al. (2012)
As fertilizer for tomato plant	K (0.76%), Ca (7.80%) and Mg (0.99%)	Cu (55.8), Fe (3810.0), Mn (208.0) and Zn (171.6)	Guatemalan Sugar Foundation (Fundazucar), Guatemala City, Guatemala	Cifuentes et al. (2013)
As an amendment material to minimize heavy metals leachability	-	Mn (0.3), Cu (ND), Zn (0.5), Cd (ND), Ni (ND), Ca (saturated), Mg (8.2) and Sr (0.2)	Sugar Mill, Malaysian Sugar Manufacturing (MSM) Sdn Bhd, Seberang Perai, Pulau Pinang.	Maheera et al. (2013)
As a bio-fertilizer for enhancing the Soil fertility	-	macro and micro nutrients	Sugar Industry, India	Nagesh et al. (2013)
As a source of plant nutrients for groundnut	N (1.54%), P (1.08%), K (2.95%), Ca (3.20%), Mg (2.00%) and Na (1.05%)	Cu (45.0), Zn (105.0), Fe (2000), and Mn (190)	Sugar Mill, Tamil Nadu, India	Selvamurugan et al. (2013)
As fertilizer in aquaculture and fish culture systems	-	Ca (16620.5556), K (6322.4259), Na (7223.3889), Mg (3478.2593), Co (36.2815), Cr (17.0389), Cu (19.3607), Fe (5293.7778), Mn (159.2237), Ni (9.5415), Zn (126.4315), Al (264.0333), Ba (43.5481), Cd (2.1219), La (1.7674), Li (14.8278), Pb (39.7685) and Sr (13.1578)	Pattoki Sugar Mills Ltd., Kasur; Brothers Sugar Mills Ltd., Kasur; Haseeb Waqas Sugar Mills Ltd., Nankana Sahib; Baba Farid Sugar Mills Ltd., Okara; Ittefaq Sugar Mills Ltd., Pakpattan; Ramzan Sugar Mills Ltd., Chiniot; Shakarganj (II) Sugar Mills Ltd., Jhang; Chaudhary Sugar Mills, Toba Tek Singh and Popular Sugar Mills Ltd., Sargodha; Central Punjab, Pakistan	This study Kashifa et al. (2016)

They applied farmyard manure, cane trash, press mud, vermicompost and biocompost in combination with recommended inorganic fertilizers and attained recorded increased cane yield over inorganic fertilizer alone, besides improving the soil fertility and economizing the cane production. Results of Nagesh et al. (2013) are also in agreement of our research since they proved that sugar press mud (SPM) is a valuable bio-fertilizer and useful to increase the macro and micro nutrient of soil thus enhancing the soil fertility. Zafar et al. (2013) determined the effect of different doses of press mud on Cu and Ni concentrations of oat fodder (Avena sativa L.) in Sargodha, Pakistan. They revealed that feeding ruminants with this fodder would be safe with no potential threat of heavy metals concentration which did not rise above the suggested reference standards. They concluded that press mud fertilizer did not show heavy metal poisonous squander; additionally proved to be a first rater source of soil elements and organic stuff to the agricultural land. Ghulam et al. (2010) assessed the application of press mud (a waste by-product from sugar factories) as organic amendment @ 15 to 20 t ha-1as the most suitable dose for improving the physico-chemical properties of calcareous soil. Khan (2011) also suggested that SPM can be considered as one of the good options to use this organic waste/bio-solid as organic fertilizer for better crop and fodder yields in an environment friendly manner and its application to agricultural soils would be sustainable and economical due to nutrient cycling and disposal of the waste on basis of his research on this sugar industry by-product.

On the basis of our research, also supported by the previous literature explained above, Press Mud can be considered as a beneficial source of essential metals allowing leaching of these into the water body of the ponds; making the medium well fertile and hence making it possible for this by-product of sugar industry to be used as an aqua-fertilizer in fish ponds. However, a further research in this context will also be conducted for evaluation and requirement of the exact amount of sugar press mud application as an aquaculture fertilizer for fish growth.

Conclusion

This research provided detailed information on the presence of elements and their correlation being significantly different from each other (P < 0.05, P < 0.01, P < 0.001, P < 0.0001). Owing to the presence of sufficient amounts of essential-macro and essential-trace elements in press mud, we recommend its future utilization as an aquaculture and agriculture fertilizer for economical stabilization using the concept of 3R formula for waste management i.e. reduce, recycle and reuse. It may be concluded that the sugar press mud, a sugar mill by-product can be considered as one of the good options for using this organic and waste bio-solid as an organic, highly valuable, futuristic naturally fortified fertilizer in intensive/semi- intensive aquaculture/fish ponds for better fish growth, yields and production in an environment friendly manner.

Supplementary material

There is supplementary material associated with this article. Access the material online at: http://dx.doi.org/10.17582/journal.pjz/2018.50.4.1329.1340

Statement of conflict of interest

Authors have declared no conflict of interest.

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