Sustainable Encapsulation of Limosilactobacillus fermentum Using Cellulose Microfiber Hydrogel: Antimicrobial and Immunomodulatory Effects Against Staphylococcus aureus
Sustainable Encapsulation of Limosilactobacillus fermentum Using Cellulose Microfiber Hydrogel: Antimicrobial and Immunomodulatory Effects Against Staphylococcus aureus
Usman Pato1*, Emma Riftyan1, Yusmarini1, Evy Rossi1, Azzahra Adeela Putri1, Shelvia Karvina1, Tri Zulia Ningrum1, Nisa Ikhwana1, Tjipto Leksono2, Aqilah Sakura Usman3, Agrina4
ABSTRACT
Probiotics, live bacteria that confer health benefits when administered in enough quantities, have garnered significant interest for their capacity to modulate the immune system. This study aimed to investigate the effects of challenging Limosilactobacillus fermentum InaCC B1295 (LFB1295) with Staphylococcus aureus on weight gain, the amount of lactic acid bacteria (LAB) and S. aureus in feces, and the immune system in rat blood serum. This research was conducted experimentally in vivo using LFB1295 cells encapsulated with cellulose microfiber hydrogel (CMFH) from oil palm solid waste (OPSW) challenged with S. aureus and measured weight gain parameters, total faecal LAB and S. aureus, and several blood serum immune parameters. The results demonstrated that rats given LFB1295 encapsulated in CMFH had lower S. aureus counts by 3.16 to 14.47%, greater body weights by 19.50 to 37.33%, and total LAB counts by 7.80 to 13.15%. Oral administration of CMFH-encapsulated LFB1295 significantly (p<0.05) influenced the immune system in the blood serum of rats. Rat blood levels of LFB1295 encapsulated in CMFH could be significantly (p<0.05) increased, and IgE level could be lowered by24.04 to 64.94% and IL-10 could be increased by 9.27 to 9.59%; however, IgA and IL-6 levels were not significantly (p>0.05) affected. Based on the results of in vitro and in vivo studies, the findings of this study are the first use of CMFH from OPSW that works well as a local probiotic encapsulant. Lb. fermentum InaCC B1295 can inhibit the growth of S. aureus which is closely related to the formation of immune responses. This probiotic has the potential to be used to maintain the health of the digestive tract and immune system in humans.
Keywords | Probiotics, Limosilactobacillus fermentum, Staphylococcus aureus, Immune system, Cellulose microfiber, Oil palm
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