The Effect of Supplementation Lignolitic Probiotic in Rice Straw for Digestibility and Efficiency of Microbial Protein Synthesis using

In Vitro Residual Gas Production

Indah Prihartini1*, Miftachi Ari2, Manar Fayiz Mousa Atoum3,4, Akhis Soleh Ismail1 and Listiari Hendraningsih1

1Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl. Raya Tlogomas 246 Malang 65144, Indonesia; 2Faculty of Animal Science, University of Brawijaya Malang, Jl. Veteran Malang 65145, Indonesia; 3Molecular Biology and Genetics, The Hashemite University, Zarqa PO Box 330127, 13133, Jordan; 4Faculty of Applied Health Sciences, The Hashemite University, Jordan.

Received | August 19, 2021; Accepted | December 15, 2021; Published | December 30, 2021

*Correspondence | Indah Prihartini, Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang, Jl. Raya Tlogomas 246 Malang 65144, Indonesia; Email: [email protected]

Citation | Prihartini, I., M. Ari, M.F.M. Atoum, A.S. Ismail and L. Hendraningsih. 2021. The effect of supplementation lignolitic probiotic in rice straw for digestibility and efficiency of microbial protein synthesis using in-vitro residual gas production. Sarhad Journal of Agriculture, 37(Special issue 1): 136-143.

DOI | https://dx.doi.org/10.17582/journal.sja/2021.37.s1.136.143

Keywords | Improve ruminant feed, Lignocelluse, Lignochloritic bacteria, Rumen microbe, Waste as feed

Introduction

Agricultural waste is an alternative source of fiber for ruminants, specifically in the dry season, which is also cheap and has a high ability. Rice straw (Oryza sativa L.) is potential waste that Indonesian people have not widely used, but only a small portion of rice straw was utilized as an energy source for ruminants. Rice straw is a low-quality feed and limited utilization due to low protein (3.47 %) and the highest lignin (6.70 %) (Prihartini et al., 2009). He et al. (2019) also reported that the chemical composition of rice straw is dry matter 28.2 %, crude protein 8.16 %, true protein 6.65 %, nonprotein nitrogen 1.52 %, neutral detergent fiber 62.6 %, acid detergent fiber 33.5 %, and water-soluble carbohydrate 1.98 %. Furthermore, the compositions of elemental activated carbon of rice straw consist of Carbon 37.93 %, Hydrogen 6.19 %, Nitrogen 0.99 %, and Sulphur 2.07 % (Saad et al., 2019)

Lignocellulose is an essential component of cell walls and forms the most significant portion of the carbon produced by plants (Prihartini et al., 2009). In the review, Guan et al. (2018) and Goodman (2020) reported that the major components of rice straw are cellulose, hemicellulose, lignin, and ash which 32.0 % to 38.6 %, 19.7 % to 35.7 %, 13.5 % to 22.3 %, and 10 % to 17 %, respectively. On the other hand, lignin of rice straw cell walls also contains silica surrounding the cell wall, and cellulose has changed from amorphous into a crystalline form. Hence, causing most carbs in the form of cellulose and hemicellulose to experirnce difficulty in digestion into energy (Prihartini et al., 2009). The form of silica (SiO2) in the rice straw is crystalline, amorphous, and silica gel (Mirmohamadsadeghi and Karimi, 2020).

Rumen consists of bacteria, fungi, and protozoa, where most bacteria and fungi have the ability to degrade cellulose. However, rumen microbes have a limited ability to degrade lignin, while cellulose is naturally bound to the lignin, limiting the use of lignocellulose. To obtain microbial protein synthesis efficiency (ESPM) high rumen, microbes need N in the form of NPN (Non-Protein Nitrogen) and easy fermented carbohydrates for energy resources and minerals.

The study from Prihartini et al. (2009) stated that three isolates of lignochloritik have high growth potential, production, and enzyme activity, and specifically degrade lignin and organochlorine, grow well in the media are natural carriers of rice straw. However, the capability of microbial into lignin biodegradation is different, which is influenced by the composition and structure of lignin in plant cell walls and the ability lignolitic oxidative enzymes produced by microbes (Prihartini and Khotimah, 2011).

Lignochloritic bacterial isolates have the nature of facultative anaerobic bacteria that lives in both aerobic and anaerobic conditions and live inside or outside the cattle body. Bacterial growth is also high, hence, it was used as probiotics and introduced into the rumen to improve the digestibility of nutrients in the rumen and the ability to digest lignocellulose material (Prihartini and Khotimah, 2011).

Probiotics are live microbes in the livestock carrier medium is advantageous because it creates balance in the digestive tract microflora. In addition, these microbes help the integrity of intestinal mucosa, the process of metabolism, and boost immunity, thereby creating optimum conditions for feed digestion and conversion efficiency to facilitate the absorption of nutrients in the process of livestock (Arowo and He, 2018; Elghandour et al., 2015).

From the description above, it is necessary to test the digestion of rice straw lignocellulose with lignolitic probiotic supplementation before being applied to livestock directly. An alternative solution to determine the digestibility of feedstuffs is through digestibility experiments. The estimation method was in vitro digestibility. It is indirectly the principle replicate the atmosphere on ruminant digestion equipment, while the measurement of gas production in vitro fermentation of organic material describing associated with Metabolizable Energy (ME), while the Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), and Efficiency of Microbial Protein Synthesis (ESPM) from the residual gas production is the actual dry matter or organic matter fermented in the rumen.

Materials and Methods

Study for gas production, dry matter digestibility analysis (DMD), organic matter digestibility (OMD), and efficiency of microbial protein synthesis (ESPM) from the residual gas production in vitro was conducted in the Nutrition Laboratory, whereas rumen fluid intake was conducted at the Field Laboratory, Sumber Sekar, Faculty of Animal Husbandry, Brawijaya University, Malang, East Java, Indonesia.

This study used rice straw IR 64 cultivar, Lignolitic probiotic, rumen fluid from fistulated dairy cattle, and materials to analyze gas production, digestibility, and ESPM in vitro. The method used in this study was Randomized Complete Block Design (RCBD) that consisted of four levels of treatments and three replications. The groupings are based on the time of rumen fluid collection. The treatments were followed:

P0 = rice straw (control)

P1 = rice straw + 0.5 % probiotic levels of rice straw DM

P2 = rice straw + 1.0 % probiotic levels of rice straw DM

P3 = rice straw + 1.5 % probiotic levels of rice straw DM

The dry matter measurement was carried out by heating the feed residue in an oven at 105 oC for 24 h,

while the organic matter measurement was carried out by burning the residue in a 600 oC furnace for 4 h. DMD and OMD are calculated by Equation (1) and Equation (2) as follows:

Which a is sample mass; b is dry matter mass, c is residue mass, d is a dry matter of residue, e is blank mass, f is a dry matter of blank mass, and g is an organic matter of sample.

Methods for measuring gas production in vitro were used to identify residues incubation or fermentation products such as microbial biomass, VFA, and gas volume. Gas production measurements were carried out 11 times, namely at the incubation period of 2 h,

4 h, 8 h, 12 h, 16 h, 24 h, 36 h, 48 h, 72 h, and 96 h.

Furthermore, estimation of microbial biomass production was conducted based on the residual degraded pseudo (apparent digestible) were reduced with pure inert residues (true digestible) treatment and resulted in Neutral Detergent Solution (NDS) (Blummel et al., 1997). This method was also carried out by Yogi et al. (2017).

All of the data were analyzed by One Way ANOVA of CRD, followed by Duncan Multiple Range Test for testing the significant level at 5 % (Adinurani, 2016). The data obtained were statistically analyzed using SPSS software version 21.0 (SPSS, 2012).

Results and Discussion

In Vitro Gas Production

The production of in vitro gas resulted from the fermentation process in the rumen and describes the number of OM digested in the rumen. The amount of gas released when the feed material by in vitro incubated in rumen fluid was closely linked to the digestibility of feed. Therefore, periodic measurement of gas production during the 96 h was performed to determine the amount of gas produced at a certain incubation time (2 h, 4 h, 8 h, 12 h, 16 h, 24 h, 36 h,

48 h, 72 h, and 96 h). The results of in vitro gas production are presented in Table 1. Meanwhile, the graphic of the relation between incubation time with gas production during the 96 h is shown in Figure 1.

The results of the variant analysis showed that lignolitic probiotic supplementation had a significant effect

(P < 0.05) on in vitro gas production at an incubation time of 2 h and 4 h. At the incubation time of 8 h, the effect was very significant (P < 0.01) and at the incubation time of more than 8 h had no significant impact (P > 0.05). The length of the incubation time of 2 h and 4 h indicates that the highest gas production is present in P3 (1.5 % lignolitic probiotic supplementation), which is not different from P1 (0.5 % lignolitic probiotic supplementation) and P2 (1 % lignolitic probiotic supplementation). The lowest gas production is in P0 (0 % lignolitic probiotic supplementation), unlike P1. An incubation period of 8 h shows that the highest gas production is in P3, which is not different from P2. Furthermore, the lowest gas production is in P0, which is not different from P1.

The level of lignolitic probiotics increases the value of gas production, indicating that the probiotic lignolitic affects fermentation in the rumen fluid. This is because lignolitik bacteria are able to degrade lignin which becomes the cell wall of rice straw, hence, cellulose and

 

Table 1: Effect of lignolitic probiotic supplementation in rice straw on in vitro gas production.

Treatment	gas production (mL mg–1 DM) on the hour incubation period
	2	4	8	16	24	36	48	72	96
P0	0.75 + 0.25b	2.23 + 0.38b	3.73 + 0.24c	9.05 + 2.00	19.09 + 5.23	33.78 + 4.63	44.68 + 3.23	57.14 + 3.49	67.38 + 5.04
P1	1.08 + 0.27ab	2.41 + 0.15ab	4.32 + 0.38bc	10.19 + 3.09	20.97 + 6.03	34.92 + 4.59	45.96 + 3.19	59.70 + 4.99	69.48 + 5.31
P2	1.33 + 0.28a	2.99 + 0.49a	5.23 + 0.65ab	10.96 + 3.11	21.74 + 5.32	36.71 + 4.57	47.33 + 3.13	62.33 + 6.63	71.67 + 6.12
P3	1.58 + 0.38a	3.32 + 0.76a	5.73 + 0.65a	12.05 + 2.88	23.38 + 5.14	38.05 + 4.48	48.93 + 3.97	62.92 + 5.05	73.86 + 6.28
P-value	0.043	0.035	0.006	0.626	0.810	0.683	0.496	0.532	0.569

 

Note: a,b,c superscript in the same column indicate significant difference (P < 0.05).

 

hemicellulose were utilized by rumen microbes optimally (Prihartini and Hariska, 2019). While P0 only use rumen bacteria, which is not able to degrade lignin of rice straw, hence, cellulose and hemicellulose cannot be utilized by rumen microbes optimally. On an incubation period of more than 8 h, lignolitic probiotic supplementation did not affect in vitro gas production. This is considered because free cellulose and hemicellulose are fermented into VFA and are used for microbial protein synthesis, such as presented in Table 3, FOM and ESPM increase with increasing probiotic levels). Izzudin et al. (2018) state that the gas produced results from the fermentation of feed, specifically organic materials, into the VFA (Volatile Fatty Acids) as an energy source in livestock are carried by the animal. This result is in line with the experiment of (Rabelo et al., 2017) that using Lactobacillus bunchneri as a probiotic increases the gas production in the rumen. Furthermore, Hassan et al. (2020) reported that using probiotics by feed enhances the gas production in the rumen.

The Figure 1 showed that gas production is high in anaerobic condition during 96 h. This identified that increasing incubation period affected gas production enhancement. The increase due to the probiotic lignolitic does not antagonize the rumen, but it digests other materials. Therefore, the addition of probiotics in feed rice straw with lignin, cellulose, and hemicellulose is expected to be easily digestible. As explained above, that probiotic lignolitic degrade lignin well (Prihartini et al., 2009), hence, the nutrition of rice straw was degraded by rumen microbial, which is why gas production is high. This is also in line as Shah et al. (2018) stated that the Ligninolytic Bacillus sp. strains break down the lignin structure on the rice straw.

Residues Digestibility of In Vitro Gas Production

Anaerobic rumen microbe that is able to digest the feed material into the rumen becomes intermediated metabolites that were absorbed, utilized by microbes for metabolic processes in the body. The ability of livestock to use or ingestion of fiber depends on the action of microbes discovered in the digestive tract. Microbial activity degrades raw fiber into organic acids such as VFA, i.e., acetic acid, propionic acid, and butyric acid. The average of DMD and OMD and in vitro gas production at 96 h incubation period is presented in Table 2.

 

Table 2: Effect of lignolitic probiotic supplementation in rice straw on DM and OM in vitro gas production on 96 h incubation.

Treatment	Digestibility (%)
	DM	OM
P0	33.80 + 0.53d	37.56 + 1.10d
P1	35.95 + 0.50c	39.93 + 0.55c
P2	40.17 + 0.16b	45.09 + 0.70b
P3	43.56 + 0.76a	48.41 + 0.27a
P-value	0.000	0.000

 

Note: a,b,c,d superscript in the same column indicate significant difference (P < 0.05).

This experiment showed that lignolitic probiotic supplementation in rice straw in vitro with a 96 h incubation period had a very significant difference

(P < 0.01) on dry materials and organic matter digestibility. The higher the dose of lignolitic probiotics, the higher the digested levels of dry matter (DM) and organic matter (OM). The highest dry and organic digestibility levels were discovered in P3, followed by P2, P1, and lowest in P0 (0 % of lignolytic probiotic supplementation).

Prihartini and Khotimah (2011) reported that lignolitic bacteria have high-potential probiotics on growth, microbial, and enzyme activity production and degrade lignin. Probiotics also increases the population and activity of cellulolytic bacteria in the rumen. The enzyme produced by lignolitic probiotics is the enzyme laccase. Laccase is an oxidizing enzyme, which was first played in the process of degradation (Prihartini and Khotimah, 2011) and an extracellular enzyme that contains protein and minerals Cu. This enzyme reduces oxygen to water and simultaneously forms a one-electron oxidizing aromatic variety. This enzyme has the ability to oxidize lignin with phenol subunits, but with the addition of a mediator, the sub-unit enzyme cannot oxidize phenol (Disale and Dixit, 2020).

Degradation of lignin in the rumen is very limited, and only a small portion of the rumen microbial compound transform, not condensed lignin. However, the presence of laccase enzyme in the probiotic lignolitic allowed the enzyme to break the bond of lignocellulose in the rumen, which increases digestibility into DM, and OM was material fermented in the rumen.

Lignolitic probiotics have a high potential to degrade lignin and low potential in degrading cellulose, thereby providing easily degraded products by rumen microbes. Microbial fermentation in the rumen, specifically in degrading fiber fraction, is determined by the production and activity of cellulolytic enzymes. A very influential factor is the availability of nutrients for microbial synthesis, which indicates the potential for higher rumen degradable due to the rumen environment conducive to the activity of rumen fermentation which leads to the DM degradation rate increased. This is because the addition of probiotics led to more stable rumen conditions and anaerobic, which led to the growth and development of the bacteria. Thus, the digestibility of feed substances, specifically dietary fiber digestibility, is increased. Astuti et al. (2018) stated that from all kinds of microbes, bacteria are the digestive extensively in the rumen. On the other hand, probiotics also enhance the intestinal architecture and immunomodulatory processes in a symbiotic and beneficial way (El-Tawab et al., 2016; Raabis et al., 2019). This indicates the higher the dose of lignolitic probiotic supplementation, the higher digested levels of DM and OM.

The efficiency of microbial protein synthesis

Dietary nucleotides had no significant measurement of microbial protein synthesis in their role to provide protein for the microbes. Synthesis of microbial protein is affected by the rate of solving the Nitrogen (N) feed, rate of absorption of ammonia, rate of the feed material flow will exit the rumen microbial amino acid requirement, and the type of rumen fermentation (Lopez and Dominguez-Ordonez, 2019; El-Nagar et al., 2019). This causes the NDS solution to digest all the microbes and plant cell walls, leaving residues that cannot be digested.

Based on Table 3, it could be analyzed that the addition of the probiotic against the value ESPM very significantly (P < 0.01). Thus, the value ESPM increased with the growing use of probiotics in rice straw because the feed containing the crude protein treatment was higher than the control diet to contribute N to microbial protein synthesis.

 

Table 3: The effect of lignolitic probiotic supplementation in rice straw on gas production , ESPM and FOM 96 h incubation.

Treatment	Gas production (mL 500 mg–1 BK)	FOM (g)	ESPM (g N kg–1 FOM)
P0	67.38 + 5.04	156.92 + 2.79d	10.95 + 0.20d
P1	69.48 + 5.31	166.17 + 2.24c	19.03 + 0.39c
P2	71.67 + 6.12	187.43 + 2.60b	27.48 + 0.31b
P3	73.86 + 6.28	202.53 + 2.73a	34.26 + 0.43a
P-value	0.569	0.000	0.000

 

Note: a,b,c,d superscript in the same column indicate significant difference (P < 0.05).

The lowest values discovered in P0 ESPM, i.e., 10.95 g N kg–1 FOM, presumably because this rice straw is a source of fiber that is not easily digested in the rumen to VFA and gas, causing its low ESPM values, whereas the highest values discovered on the P3 ESPM, i.e., 34.26 g N kg–1 FOM. This fact indicates a balance of VFA production as an energy source and NH3, which was used for microbial protein synthesis. High bacterial biomass was influenced by probiotics lignolitik, which increases the microbial population needed to produce bacterial growth factors such as malic acid and increase pH stability that supports the growth of rumen cellulolytic bacteria (Prihartini et al., 2009). Microbial protein synthesis accounted for 59 %

of amino acids in the small intestine than amino acids that are not degraded to amino acids to satisfy the needs of livestock to produce faster.

Microbial protein production was straight with activity and fermentation products, particularly NH3 and VFA rumen. Based on Table 3, ESPM production value is proportional to the study of Prihartini and Hariska (2019), namely the value of NH3 and VFA on feed treated with rice straw was added lignolitic probiotic increase than the control diet. There was a relationship of mutual benefit between the rumen microbial population and the digestibility of microbial protein production of nutrients in the rumen. Increasing rumen microbial population with high activity causes more nutrients to be digested. In contrast, the results of rumen digestion or fermentation products such as NH3 and VFA as an input for the formation of body cells which in turn ensures microbial growth and activity of microbes themselves (Lopez and Dominguez-Ordonez, 2019).

The addition of lignolitic probiotics improves digestibility nutrients which means the protein contained in feed ingredients will also rise. It is characterized by increasing the concentration of rumen NH3 further positive impacts on microbial protein production. As stated by Prihartini et al. (2009), because the rumen microbes cannot use amino acids directly, feed protein will be degraded further into the rumen NH3 and will use these as a source of nitrogen for synthesis body cells. Increased rumen microbial protein production means the supply of protein was used to host productivity (livestock).

Conclusions and Recommendations

This study concludes that lignolitic probiotic supplementation may improve rice straw digestion characterized by increased gas production in vitro at an incubation length of 2 h, 4 h, and 8 h of incubation. In addition, lignolitic probiotic supplementation may also improve the digestibility of dry ingredients, organic matter, FOM, and ESPM of rice straw.

Novelty Statement

The novelty of this study is an ability of lignolitic probiotic high lignin degraded in an aerobic condition show with (i) increased gas production, (ii) increased dry matter and organic matter digestibility, (iii) increased efficiency of protein microbial synthesis feed based on rice straw on in vitro residual gas production test.

Author’s Contribution

IP: Conceptualized and designed the study, elaborated the intellectual content, and performed the literature search, data acquisition, statistical analysis, manucript preparation, and manuscript revision.

MA: Carried out experimental studies and manuscript reviews.

MFMA: Elaborated the intellectual content, performed literature search, data manuscript review and guarantor.

ASI and LH: Reviewed manuscript.

All authors read and approved the final manuscript

Conflict of interest

The authors have declared no conflict of interest

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