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The Protective Effect of Ellagic Acid on Cyclophosphamide Induced Renal Damage in Rats

The Protective Effect of Ellagic Acid on Cyclophosphamide Induced Renal Damage in Rats

Muhammad Nasir Bhaya1,2* and Hikmet Keles2

1University of Veterinary and Animal Sciences Lahore, Pakistan
2Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03030, ANS Campus Afyonkarahisar, Turkey
 
* Corresponding author: dr.muhammadnasir399@gmail.com

Fig. 1.

Histopathological appearance of kidney tissues of all groups, HE staining, scale bar= 150 µm A, Control group: normal kidney morphology. B-C, CP group: hyperemia, epithelial degeneration, swelling, cystic dilatation, proteinaceous material in tubuli, congested and hypercellular glomeruli, inflammatory cells infiltration in cortical and medullary area. Arrows pointing events; h: hyperemia, c: congestion, d: degeneration, i: inflammation, p: proteinaceous material, cd: cystic dilatation. 1D, CP+EA50 group: significant decrease in pathological lesions. 1E, CP+EA75 group: more protective effect and normal architecture of kidney.

Fig. 2.
8-OHdG evaluation: kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group is not showing specific positivity. B, CP group is showing specific cytoplasmic and luminal positivity of tubuli. C, CP+EA50 group is showing mild positivity. D, CP+EA75 group is showing quite improved results of less specific positivity.
Fig. 4.

Bcl-2 positivity evaluation kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group revealed specific cytoplasmic and luminal positivity of tubuli. B, CP group revealed less specific positivity. C. CP+EA50 group revealed mild positivity. D, CP+EA75 group revealed specific positivity like control group.

Fig. 3.

HIF-1α evaluation: kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group revealed no positivity. B, CP group revealed specific positivity of tubuli. C, CP+EA50 group revealed mild positivity. D, CP+EA75 group revealed no positivity like control group.

Table I.

Grading of histopathological lesions in all groups.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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