The Protective Effect of Ellagic Acid on Cyclophosphamide Induced Renal Damage in Rats
Muhammad Nasir Bhaya1,2* and Hikmet Keles2
1University of Veterinary and Animal Sciences Lahore, Pakistan
2Department of Pathology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03030, ANS Campus Afyonkarahisar, Turkey
* Corresponding author: dr.muhammadnasir399@gmail.com
Fig. 1.
Histopathological appearance of kidney tissues of all groups, HE staining, scale bar= 150 µm A, Control group: normal kidney morphology. B-C, CP group: hyperemia, epithelial degeneration, swelling, cystic dilatation, proteinaceous material in tubuli, congested and hypercellular glomeruli, inflammatory cells infiltration in cortical and medullary area. Arrows pointing events; h: hyperemia, c: congestion, d: degeneration, i: inflammation, p: proteinaceous material, cd: cystic dilatation. 1D, CP+EA50 group: significant decrease in pathological lesions. 1E, CP+EA75 group: more protective effect and normal architecture of kidney.
Fig. 2.
8-OHdG evaluation: kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group is not showing specific positivity. B, CP group is showing specific cytoplasmic and luminal positivity of tubuli. C, CP+EA50 group is showing mild positivity. D, CP+EA75 group is showing quite improved results of less specific positivity.
Fig. 4.
Bcl-2 positivity evaluation kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group revealed specific cytoplasmic and luminal positivity of tubuli. B, CP group revealed less specific positivity. C. CP+EA50 group revealed mild positivity. D, CP+EA75 group revealed specific positivity like control group.
Fig. 3.
HIF-1α evaluation: kidneys stained with Streptavidin biotin peroxidase complex method with AEC chromogen and Mayer’s Hematoxylin counterstain, scale bar=150 µm. A, Control group revealed no positivity. B, CP group revealed specific positivity of tubuli. C, CP+EA50 group revealed mild positivity. D, CP+EA75 group revealed no positivity like control group.
Table I.
Grading of histopathological lesions in all groups.