Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
... results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the BMI....
Mousa O. Germoush1,*, Mohsen G. Al-Mutary2, Ahmad R. Al-himaidi3, Muath G. Al-Ghadi3, Daisaku Iwamoto3,4, Yousef Al-anazi3, Aiman Ammari3, Javed Ahmad3,5, Abdulaziz Al-Khedhairy3,5
... significant increase in blastocyst development when compared with other groups. Gene expression analysis revealed a significant increase in BAX expression in groups supplemented with SFF 20% and 40%, whereas the expression of BCL-2 was significantly increased only in the 40% SFF supplemented group. The ratio of Bax to Bcl-2 was significantly increased in the embryos derived from oocytes matured in 20% and 40% SFF supplemented media. HSPB1 expression was signi...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
...tal rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 ± 3.5% vs. 64.6 ± 1.8%, and 24.8 ± 2.2% vs. 15.9 ± 4.3%, respectively). However, blastocyst cell number was not different between groups...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...ighest cleavage rate and blastocyst rate of porcine parthenogenetic embryos, which was significantly higher than Group I with intact oocytes (P<0.05) and Group III with the puncture of zona and plasma membrane and aspiration egg cytoplasm (P<0.05) on the cleavage rate, and was extremely significantly higher than Group III on the blastocyst rate (P<0.001). Raman spectroscopy of the spent culture media of these parthe...
Lei Gao1, Gong-xue Jia2, Zheng-yuan Huang1, Ming-xing Yue3, Chao Zhang1, Shi-en Zhu1 and Xiang-wei Fu1,*
...ytes, 2-cell embryos and blastocysts. The cytoplasmic maturation of MII oocytes and blastocysts in aged mice were significantly compromised to those of young mice (P < 0.05). Furthermore, GV oocytes, 2-cell embryos and blastocysts showed significantly higher relative intensities of pi-H2AX in aged mice (P <0.05). Taken together, our result indicate that aging disturbed oocyte maturat...
Zhi-Peng Li, Laiba Shafique, Saif ur Rehman, Kui-Qing Cui, Xiao-Can Lei, Xing-Rong Lu, De-Shun Shi* and Qing-You Liu*
...ared and elevated in the blastocysts stage. The following western blot analysis revealed a similar expression pattern with QRT-PCR and immunofluorescence. In conclusion, the expression pattern of Gas6 gene is closely related to oocytes maturation and embryo development. Gas6 may play a crucial role in the process of oocytes maturation and improve the development potential of early embryos by promoting the cytoplasmic maturation.
... 
Dwi Budiono1,5, Ratih Rinendyaputri2, Ariyani Noviantari2, Mokhamad Fahrudin3, Ni Luh Putu Ika Mayasari4, Vista Budiariati1,6, Diah Nugrahani Pristihadi1,3, Noer Muhamad Dliyaul Haq1, Arief Boediono3*
...p;...
Chalothon Amporn1, Somchit Guntaprom1*, Sarawut Duongmawong1, Wilasinee Srisanyong1, Sirikanda Thanasuwan1, Phalita Koonnadilokpot1, Dechawut Bunyaluk2, Juggrid Jugsumrit3, Jakrit Yaeram4, Chompunut Lumsangkul5
...8-16 cells developments, blastocyst in 100 µM cysteine group (73.00%, 54.79%, 30.14%, and 17.81%, respectively) were higher than the control group (48.00%, 37.50%, 18.75%, and 10.42%, respectively) and 200 µM cysteine (respectively, 52.00%, 38.46%, 19.23%, and 9.62%) at a significance level of 0.05, but the difference between the 200 µM cysteine group and the control was not significant (p>0.05). The findings confirm that adding 100 µ...
M.A.M.M. Shehab-El-Deen1,2*, S.A. Al-Dobaib1 and K.A. Al-Sobayil1
Jie Wang1, Di Fang1, Jianqing Zhao1, Fei Huang2, Bo Liu2, Weikun Tao2, Baoshan Cui2 and Qinghua Gao1,2,3*
...mbryo, morula XY embryo, blastocyst XX embryo, and blastocyst XY embryo. A sequencing library was constructed by the Smart-Seq2 amplification. The transcriptome was sequenced by Illumina HiSeqXten high-throughput sequence technology, and effective sequences were analyzed by functional annotation and related bioinformatics analysis. We found that Q30 percentage range of eight samples was 91.79-92.37%. The filtration sequence ...
Fadime Eroglu1*, Altan Akineden2, Cemal Cicek3, Selcuk Turkel2
Shorouq Mohamed Omara1, Ibrahim Saad El-Shamaa1, Emad Abd-Elaziz Abd-Allah2, Assmaa Abd-Allah Fathy3, Essam-Eldin Tharwat4, Mohammed Hamdy Farouk5* and Ibrahim Mahmoud Abd El-Razek1*
... buffaloes. We evaluated blastocyst and apoptosis rates in order to substantiate the potential development of cumulus oocytes–complexes (COC) and determine the effect of LC in reducing the occurrence of apoptosis under in vitro maturation (IVM) conditions for buffalo blastocysts. The tissue culture media included a control culture medium and other three LC supplemented media (2, 3 or, 4 mM LC). All LC treatments had hi...
