Kang-Seuk Choi
... The VLPs show intrinsic immunogenicity and a high safety profile. Thus, VLPs are considered one of the most promising approaches to vaccine development and are an alternative to inactivated IBD vaccines. In addition, VLP technology has many applications. This paper reviews the potential of VLPs as an alternative to IBD vaccines, along with their specific applications in the field....
Hafiz Muhammad Tahir1, Khanum Zahra2, Arooj Zaheer2, Khizar Samiullah3
...ing ability and very low immunogenicity. In this review structure and types of silk producing gland, spinning process, chemistry of spider silk fiber, mechanical properties and applications of silk in health, military industry and many other fields is discussed. Its antifungal and antimicrobial potentials are also explored. Researchers are trying to introduce advanced methods to manufacture spider silk artificially to enhance silk production. Silk fiber can be...
Basit Zeshan1,2,*, Mushtaq A. Saleem2,Javed Iqbal Wattoo2, Mohd Mokhtar Arshad1 and Maizan Mohamed1
...m the culture media. The immunogenicity of the protein was studied in an animal experiment on mice, in which mice were injected subcutaneously. These findings suggest that the truncated Sf200 expressed in the pET-32a (+) prokaryotic vector can be used as antigen to detect antibodies against IBV.
...
Lotta Wahldén1, 3, Ulf Emanuelson1, Torsten Møller2 and Jonas Johansson Wensman1*
Mumtaz Ali Khan1, Aneela Zameer Durrani1, Sher Bahadar Khan2, Shehla Gul Bokhari1, Ikramul Haq1,*, Imdad Ullah Khan3, Naimat Ullah4, Naimat Ullah Khan4, Kashif Hussain1 and Azmat Ullah Khan2
... Institute Peshawar. The immunogenicity of toxoid vaccines and bacterin toxoid vaccine were studied and compared initially in rabbits and then in goats and sheep. Experimental trials were conducted on 54 healthy animals of each species and were divided into three equal groups (n=18). The antibody titers were evaluated with indirect haemagglutination test. The results indicated significantly higher (P< 0.05) immune titer in animal group vaccinated with toxoi...
Fangxiao Dong1,2, Xuan Luo1,2, Delu Yuan1,2, Aixin Liang1,2* and Liguo Yang1,2*
...eptide obtained stronger immunogenicity, and better potentials of promoting growth and lactation, indicating that tPA is an effective signal peptide for enhancing the immune efficacy and performance of somatostatin DNA vaccine.
... 
Ghulam Akbar1*, Muhammad Anjum Zia1, Ali Ahmad2, Neha Arooj1 and Shahneela Nusrat1
Soad, E. Elsayed 1, Mohammed A. Shalaby2, Ausama A. Yousef2, Abu bakr A. Agor1,
Sherouk E. Aly1
...ion test showed enhanced immunogenicity of
developed formula when compared to the conventional one. Challenge test revealed high protection
levels that reached 90% of the Guinea pig vaccinated by nano emulsion.
Conclusion: Our findings suggest that we aimed to enhance immunity through nano emulsion of
Montanide ISA 206 is well achieved.
...
Shourok E. Aly 1, Karim Zaki1, Soad E. Elsayed2, M. Elgamal2, Ausama A. Yousef1, Mohamed A. Shalaby1
... we aimed to enhance the immunogenicity of the conventional vaccine by
developing a smaller particle size vaccine formulation through ultrasonic dispersion of aluminum
hydroxide adjuvant.
Methods: This was achieved by ultrasonication of aluminum hydroxide gel in the presence of
trehalose as dispersant and measuring different physical characters including particle size, Zeta
potential, loading capacity...
Lin Huang1, Ling Mai2, Keyan Zhong1 and Xinjun Chen3*
...ing that SeFABP had good immunogenicity. Its secondary structure contains 2 α-helix (13.07%), 10 β-sheet (56.92%) and 30% random coli. The comparison of multiple FABPs sequences showed that the species were identical and highly conserved at multiple AA sites. Specifically, SeFABP has the highest similarity with T. multiceps and M. vogae, close to 37%, but the lowest similarity with F. hepatica and F. gigatica, close to 22%. Plasmid double enzyme dig...
Mirtneh Akalu1,2*, Takele Abayneh2, Esayas Gelaye2, Behailu Tefera2, Teferi Degefa2, Vemulapati Bhadra Murthy1
Bilal Ahmed Shah1, Muhammad Avais1*, Jawaria Ali Khan1, Masood Rabbani2, Aftab Ahmad Anjum2, Muhammad Asad Ali2, Muhammad Awais1, Sohail Ahmad3 and Shahan Azeem2*
...ion for antigenicity and immunogenicity and evaluated for safety, side effects, challenge protection assay, sterility and humoral response in laboratory animals. Safety test showed no general adverse reactions to the vaccines when injected either into mice or rabbits or cow calves. Challenge protection studies revealed a significantly higher survival rate in vaccinated mice and rabbits compared to placebo groups. None of the vaccines when streaked onto culture...
Nguyen Thi Thu Hien1, Dong Huu Rin2, Nguyen Xuan Hoa1, Le Viet Tuan Khanh2, Phung Thang Long1*, Dinh Thi Bich Lan1
...erformed to evaluate the immunogenicity of M. hyopneumoniae P36 protein for use as an antigen to develop novel vaccines and bio-products (e.g., egg yolk antibody) against M. hyopneumoniae.
Keywords | Mycoplasma hyopneumoniae, porcine enzootic pneumonia, P36 protein, protein expression, recombinant protein, antigenicity
...
Abida Mushtaque1, Ali Ahmad Sheikh1, Aamir Ghafoor1*, Wasim Shehzad2 and Nadeem Ahmad3
...tting. Once purified the immunogenicity of rOmpH was evaluated in BALB/c mice. The expression of recombinant OmpH protein (rOmpH) resulted in its accumulation in cytosol. The purified rOmpH protein had a molecular weight of 36 kDa and concentration around 0.973 mg/mL. The BALB/c mice group injected with 100 ng of purified rOmpH exhibited significant protection (80%) when challenged with LD50 of P. multocida B:2. From the obtained results it is evident that rOm...
Saira Jabeen1, Muhammad Avais1*, Jawaria Ali Khan1, Kamran Ashraf2, Aftab Ahmad Anjum3
...vaccine preparation, the immunogenicity of this E. coli (aggR) strain was determined across various concentrations in a cohort of 20 locally bred rabbits. For preparation of vaccines, the antigen concentration was adjusted to 10^10 cells/mL, a level determined to trigger highest IHA antibody response in rabbits. Three distinct vaccine types were prepared, each employing different adjuvants. To ensure the quality and safety of these vaccines, quality control as...
Amira H. El-Ashry*
...ular dystrophy. However, immunogenicity; off-target effect,
and efficient delivery systems withstand against its spread in the clinical applications until
introducing an improvement. The aim of this review was to summarize how the various
CRISPR systems work; their important medical applications, and their limitations.
...
Asmaa Alrobai1*; Jehan Alrahimi1,2; Sahar El Hadad1,2,3
Novel Research in Microbiology Journal (2021), 5(6): 1463-1479
...ate vaccines improve the immunogenicity potential, to prevent meningococcal disease. Several previous studies have documented the decrease in post-vaccination effectiveness induced by the meningococcal vaccines. However, effectiveness requires revaccination after a period of time from the first vaccination. The purposes of this article were to provide an overview of the meningitis disease, and demonstrate the effectiveness of the meningococcal conjugate vaccin...
I Wayan Wisaksana Yasa1, I Wayan Teguh Wibawan2, Okti Nadia Poetri2*
...te the pathogenicity and immunogenicity of the IBD virus 098 Bogor ‘19 as a candidate for IBD live vaccine master seed through the bursal-body-weight ratio (BBWR), index of bursal-body-weight ratio (IBBWR), bursal lesion scoring (BLS), and IBD antibody titre evaluation. The IBD master seed candidate should be safe and have good immunogenicity responses. Thirty Specific Pathogen Free (SPF) chickens were divided into thr...
