Efficacy of Beta Vulgaris L. Ethanol Extract Treating Diabetic: An in Silico and in Vivo Study in Rat Model
Efficacy of Beta Vulgaris L. Ethanol Extract Treating Diabetic: An in Silico and in Vivo Study in Rat Model
Manan Kumar1, Sony Eka Nugraha2*, Nabila Nabila3
ABSTRACT
The purpose of the study was to examined the antidiabetic efficacy of Beta vulgaris L. ethanol extract using in vivo and in silico methodologies. Phytochemical analysis was conducted through qualitative screening with specific tests for key bioactive compounds: Mayer’s, Dragendorff’s, and Bouchardat’s reagents for alkaloids; hydrochloric acid with magnesium powder for flavonoids; and the foam test for saponins. The Microwave-Assisted Extraction (MAE) method was used to extract compounds from the beetroot tubers. Furthermore, diabetic rats were induced via a single intraperitoneal injection of streptozotocin at a dose of 65 mg/kg body weight (BW), which was administered 15 min after the administration of nicotinamide at 110 mg/kg body weight. Diabetic rats were subsequently administered varying doses of Beta vulgaris L. ethanol extract (100, 200, and 400 mg/kg BW) for 21 days, following which blood samples were obtained to evaluate hematological parameters, and statistical analysis was conducted using ANOVA followed by Tukey’s Multiple Comparison Test to determine significance (p < 0.05). Phytochemical screening showed that the betroot extract contained flavonoids, alkaloids, saponin, tannins, and triterpenoids. The in vivo results indicated that all treatment doses significantly decreased blood glucose levels (p< 0.05) compared with the untreated control group, exhibiting a dose-dependent manner. Furthermore, hematological parameters demonstrated significant improvement (p < 0.05) compared to those in the untreated control group. Moreover, in silico analysis showed that the key compounds in Beta vulgaris L. demonstrated stable binding affinities with glucokinase,while toxicity examination also affirmed the safety of the identified compounds. In conclusion, Beta vulgaris L. ethanol extract has potential as an antidiabetic medication.
Keywords | Antidiabetic, Beta vulgaris L., Extract, Hematology, In silico, In vivo
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