Pathogenicity and Immunogenicity Study of Infectious Bursal Disease 098 Bogor ’19 Virus Isolate as a Master Seed Candidate for Live IBD Vaccine
Pathogenicity and Immunogenicity Study of Infectious Bursal Disease 098 Bogor ’19 Virus Isolate as a Master Seed Candidate for Live IBD Vaccine
I Wayan Wisaksana Yasa1, I Wayan Teguh Wibawan2, Okti Nadia Poetri2*
ABSTRACT
Infectious Bursal Disease (IBD) remains a significant threat to the poultry industry, cause 40-50% mortality rate among the flock. Vaccination remains an effective strategy for preventing infection, thus making safe and potent IBD vaccines essential. This study aims to evaluate the pathogenicity and immunogenicity of the IBD virus 098 Bogor ‘19 as a candidate for IBD live vaccine master seed through the bursal-body-weight ratio (BBWR), index of bursal-body-weight ratio (IBBWR), bursal lesion scoring (BLS), and IBD antibody titre evaluation. The IBD master seed candidate should be safe and have good immunogenicity responses. Thirty Specific Pathogen Free (SPF) chickens were divided into three groups of 10 chickens each. Group 1 was inoculated with the IBD virus 098 Bogor ‘19, Group 2 was inoculated with the very virulent (vv) IBD virus 078 Lampung ‘18, and Group 3 served as the control group. The challenge virus titre was 102,7 EID50/ dose (0.3 ml) administered via oral route. Clinical signs were observed for 21 days post-inoculation . Serum samples were collected on days 0, 7, 14, and 21 post-inoculation , and antibody titres were measured using an enzyme-linked immunosorbent assay (ELISA). The BBWR, IBBWR, and BLS values were evaluated at 21 post-inoculation . None of the chickens in Group 1 showed clinical symptoms or mortality during the observation period, while Group 2 exhibited a 30% mortality rate. The average antibody titre in Group 1 was significantly higher (P<0.001) than in Group 2. BBWR, IBBWR, and BLS values in Groups 1 and 2 indicated infection leading to bursa atrophy. In conclusion, the IBD 098 Bogor ‘19 is safe and immunogenic which make it a promising candidate for an IBD intermediate-plus vaccine master seed.
Keywords | Infectious bursal disease (IBD), Pathogenicity, Immunogenicity, Live vaccine, Poultry vaccination, Intermediate-plus
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