Shakeel Ahmed* and Sarwat Jahan
...s was observed after NMS injection in both normal and metabolically stressed conditions. NMS administration induced a significant (P<0.01) increase in resistin levels, while suppressed leptin (P<0.05) secretion in both fed and 48-hrs fasting conditions. In conclusion our study suggested that NMS has a role in regulation of adipokines secretion. Its inhibitory effect on leptin and stimulatory effect on resistin shows an important relationship between NMS ...
Wenmin Cheng1,2*, Weirong Pan1,2, Yubo Qing1, Yingchao Liu1, Xingqin Zha1,2, Yan Huang2, Jige Xin1,2, Hongjiang Wei1 and Yangzhi Zeng2
... the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos...
Abdul Mannan Babar1* and Abdul Hannan Nagi2
...ing Growth Factor Beta 1 injection, and then skin punch excision on rabbit ear. In the present follow-up study consisting of eight refind techniques, best Keloid was produced by the technique where Transforming Growth Factor Beta 1 injection was followed by ventral skin, ventral perichondrium, cartilage, and dorsal perichondrium punch excision, leaving behind only dorsal skin....
Yi-qiang Ouyang1, Zhen-xin Liang1, Shi-wen Huang2, Ying Zhang3, Shao-shi Luo1, Jin-ning Liang1, Jia-fu Li1, Yu He4 and Song-chao Guo1,*
...transcriptome induced by injection of the three AD agents. These results open the door to new small-animal studies of AD that complement and extend studies in rodents and primates.
...
Jun-Ying Liu, He-He Liu*, Jie Kou, Yang-Mei Zhao, Ji-Wen Wang, Liang Li and Xiao-Hui Du
... implemented through LPS injection in Peking ducks. The results from this assay suggested that LPS injection significantly increased the concentration of tumor necrosis factor-α (TNF-α) in bursa of Fabricius (P<0.05), along with an increase in the concentrations of immunoglobulin-G (IgG) and interleukin-12 (IL-12) in the serum of ducks at 1 day and 7 days after LPS injection
Syeda Madiha, Zehra Batool, Saiqa Tabassum, Laraib Liaquat, Sadia Sadir, Tahira Perveen and Saida Haider*
...fore 15 days of rotenone injection. The effects of curcumin pre-treatment were evaluated by different motor behavioral parameters (pole test, Kondziela’s inverted screen test, inclined plane test, open field test, beam walking test and footprint test). Biochemical and neurochemical alterations were also monitored. Pre-treatment with curcumin reversed the motor impairments, biochemical and neurochemical alterations which were produced by rotenone. Further...
Ashraf Sayed Awaad1 and Mohamed Zaki Fathy2*
... a new site for epidural injection in goats based on the anatomy of the lumbosacropelvic region and confirmed by computed tomography (CT) as well as successful application of the needle within the epidural space guided by ultrasonography (US). This study was conducted on seven lumbosacropelvic regions of goat cadavers and five live goats of both sexes ranged between 25-30 kg body weight. Longitudinal and cross anatomical sections were done on these samples; th...
Muhammad Shaheer1*, Asad Aslam Khan2, Nasir Ahmed3, Tehseen Mahmood Mahju4, Ummarah Rasheed5
...ients that underwent pre-injection and one-month post-injection OCT for RNFL thickness. OCT was performed by researchers and findings were recorded accordingly. The retinal nerve fiber layer thickness was measured in superior, inferior halves and total average thickness. Pre- and post-injection OCT were conduced and macular thickness were documented.
Results:The mean ret...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi2*, Zillay Mariam1, Nadeem Sheikh1,2*
...y. After 24 hours of the injection, animals were euthanized and blood was drawn for further studies. Data were analyzed using one way ANOVA. Serological analysis of both Group 1 and Group 2 showed a significant decrease of various components including Cl- (P=0.0023), K+ (P=0.0002), Na+ (P=0.0001), total proteins (P<0.0001) and albumin (P<0.0001) when compared against control. Similarly, hematological analysis revealed a s...
Ashraf M. Mounir, A.N. El shahat* and A.M. Abdul Azeem
.../kg/day) prior to injection of ISO provide significant cardio-protective effects evidenced by an obvious reduction in the level of cardiac marker enzymes, inflammatory factors and lipid contents with marked improvement in the cardiac antioxidant status and reduction of lipid peroxidation relative to untreated infarcted group. The study concluded gamma irradiation could be used as an efficient method for sterilization and increasing the active contents o...
Muhammad Babar Khawar, Nadeem Sheikh*
Effect of paper industry leachate on various serological indices and serum proteins of wistar rats
... through intraperitoneal injection. After 24h of the injection, all the animals were sacrificed and blood was collected, sera were separated and processed for further analysis. Serological analysis revealed that leachate induction leads to significant positive variations in level of serum aspartate aminotransferases (AST) (P<0.0001), cholesterol (P<0.0001) and High density lipoproteins (HDL) (P<0.0001) while a signi...
Muhammad Babar Khawar1, Muddasir Hassan Abbasi1, 2, Sana Fatima3, Khawaja Abdul Mujeeb2, Nadeem Sheikh1
Alterations in proteins and transaminases activity induced by thioacetamide in albino rats
...ity
analysis. TAA injection resulted in significant alterations in serum proteins and transaminases levels. Significant
hypoproteinemia and hypoalbuminemia were observed in sera as well as liver tissue of both experimental groups
compared to control. Furthermore, transaminases activity was significantly increased for both; AST (P=0.0045) and
ALT (P=0.0015) in both experimental groups when compared to control. Taking in account these...
analysis. TAA injection resulted in significant alterations in serum proteins and transaminases levels. Significant
hypoproteinemia and hypoalbuminemia were observed in sera as well as liver tissue of both experimental groups
compared to control. Furthermore, transaminases activity was significantly increased for both; AST (P=0.0045) and
ALT (P=0.0015) in both experimental groups when compared to control. Taking in account these...
Faheem Ali1*, Muhammad Naeem Arbab1, Abdul Basit2, Muhammad Kashif Khan1, Gulzar Ahmad1 and Muhammad Amir1
...ensing, controlling, and injection of VARs based on the actual requirement. Such a solution is proposed in this paper along with simulation results. This would help agriculture-based industries in avoiding paneities, which would in turn lower the production cost of agricultural products. Also, sensitive equipment would not be encountering voltage fluctuations anymore ...
Yan-Guo Han1, Xiao-Li Peng1, Kai Li1, Yu-He-Tian Zhao1, Xun-Ping Jiang2, Guang-Xin E1, Yong-Ju Zhao1, Jun-Hua Ye3, Li Xu3, Qin-Tao Zhao3 and Yong-Fu Huang1,*
...mbs.
... 
Muhammad Aslam Rajput1,3, Imtiaz Ahmed Khan2, Rehana Naz Syed3 and Abdul Mubeen Lodhi3*
...nfestation, spraying and injection methods for the establishment of smut disease. It appears that disease expression was significantly influenced by pathogen inoculation method. The infection caused by S. scitamineum and subsequent whip smut development has also been greatly varied with the inoculation method. Injection method followed by wound+paste method and dipping method produced maximum disease incidence, highest numbe...
Dilshad Ahmad1* and Muhammad Afzal2
... feasible with financial injections in this sector. The study in hand has focused the role of credit in the cotton production of southern Punjab. Simple Random Sampling technique was used in the study for selecting the respondents in both districts of cotton area. Pre-tested questionnaire was developed for the collection of data 320 cotton farmers’ respondents for the year of 2015-16 including 160-credit user and 160 non-credit users. The study employed ...
Junli Sun1,2,3, Lin Bai1 2, Xiaogan Yang1,2, Yangqing Lu1,2, Shengsheng Lu1,2,* and Kehuan Lu1,2,*
...v>Intracytoplasmic sperm injection (ICSI), as the savior of male infertility, has been applied to artificial assisted reproduction (IVF). The oocytes are inevitably ruptured by the microinjector in the process of microoperatin. To study the effects of mechanical damage from microopetations on embryo development, we analyzed 60 the spent culture media of porcine parthenogenetic embryos experienced different types of microopetation using Raman spectroscopy. The ...
Shou-Hong Zhang1*, Ying Zhang1, Kun-Ming Wen2, Wei Wu3 and Wen-Yan Li3
...stered through tail-vein injections. The gene therapy efficacy was evaluated in vivo. Before and after treatment the rats were examined with MR-PWI. The perfusion parameters of PWl were compared with the fibrosis stage. The total fibrous tissue in the liver of rats treated with HGF was lower than that in control group. The time-signal intensity curve (TIC) peak of liver parenchyma of rats with hepatic fibrosis was characterized by a slow wash-in –...
Olufisayo Bamidele* and Safiriyu Idowu Ola
...mples were collected pre injection and at 60 and 120 minutes post insemination. Data recorded were Plasma LH levels, kindling rate, total kit born, total born alive, still born, weight of kits at birth and gestation period (days) and were analyzed using Analysis of variance of the General Linear Model procedure and t-test where appropriate. The plasma LH for the AI groups peaked at 60mins while it peaked at 120mins for the control. Kindling rates were 10%, 10%...
Jam Nazeer Ahmad1,2*,Mujahid Manzoor1, Zubair Aslam3 and Samina Jam Nazeer Ahmad1, 2
...ginous through micro-injection. Gene expression and proteomic study confirmed the down regulation of P450 gene and associated protein in dsRNA treated population. The application of ds RNA specific for CYP450 also reduced insecticide resistance in R. ferruginous. The developmental parameters were highly affected in RPW treated with dsRNA as compared to control samples treated with water or dsGFP. These results support the RNAi application as ...
Saeed Murtaza1, Abdul Sattar1*, Nasim Ahmad1, Muhammad Ijaz2, Maqsood Akhtar3 and Muhammad Shahzad4
... know effect of oxytocin injection on cervix, uterus and milk composition during postpartum interval in Nili-Ravi buffalo, a study was conducted at Buffalo Research Institute Pattoki, Pakistan from September to October. For this purpose, 25 animals were randomly divided into three groups: group-1 (Control; n=8), group-2 (Low dose 10IU; n=8) and group-3 (High dose 30IU; n=9) while one animal excluded at milk sampling from group-3 due to unavoidable circumstance...
Asim Faraz1*, Abdul Waheed1, Muhammad Mudasser Nazir2, Aneela Hameed3, Nasir Ali Tauqir4, Riaz Hussain Mirza1, Hafiz Muhammad Ishaq1, Rana Muhammad Bilal5
... ejection inhibition. OT injections cause increased OT blood levels which results in the prolonged myoepithelial and alveolar contractions that ultimately increase milk yield. Milk composition especially protein, fat, lactose and mineral concentration are influenced by exogenous OT administration. It affects the cell maintenance and mammary metabolism along with its proven physiological role in the milk ejection reflex. OT effects are not manifested through ef...
Murtala Muhammad, Ting Zhang, Siyu Gong, Jing Bai, Jiansong Ju, Baohua Zhao* and Dong Liu*
...mined by intraperitoneal injection of the etiological agent to the healthy A. sinensis. Physiological and 16s rRNA molecular analysis identified Streptococcus iniae as the causative agent of the disease outbreak. The bacteria has a unique biochemical profile compared with most of the previously isolated strains. Antimicrobial susceptibility tests revealed that S. iniae HNM-1 is resistant to most of the important antibiotics, including...
Joseph Anejo-Okopi1*, Ocheme Julius Okojokwu1 and Onyemocho Audu2
...venous drug addiction or injection drug use accounts for the majority of many cases apart from hemodialysis patients, unprotected sexual intercourse, blood transfusion and vertical transmission. Therefore, there is a need for government commitment for HCV elimination program through making the DAAs available and its affordability to general population to curb the catastrophic cost of managing the epidemic. However, the diagnosis of HCV infection is faced with ...
Younis Jamal1, Asad Naeem Shah1, Wasif Amin Butt1, Ahmad Naveed1*, Muhammad Usman1
EXPERIMENTAL INVESTIGATION OF THE EFFECTS OF FUEL INJECTION PARAMETERS ON DIESEL ENGINE PERFORMANCE AND EMISSIONS
...studies of changing fuel injection and other constraints of compression ignition (CI) engines for the abatement
of exhaust emissions. In current study, a direct injection (DI), CI engine was run on a test bench for the performance
and emission analyses using different nozzles and injection timings. During the experiments, two types of nozzles
known as sac and valve cov...
of exhaust emissions. In current study, a direct injection (DI), CI engine was run on a test bench for the performance
and emission analyses using different nozzles and injection timings. During the experiments, two types of nozzles
known as sac and valve cov...
Murad Ullah Khan1*, S. Zuhra2, M. Alam3, R. Nawaz4
SOLUTION TO BERMAN’S MODEL OF VISCOUS FLOW IN POROUS CHANNEL BY OPTIMAL HOMOTOPY ASYMPTOTIC METHOD
...r is based on Suction or injection through the wall, so for different values of Reynolds, we
obtained different types of semi-analytic solutions by OHAM. ...
obtained different types of semi-analytic solutions by OHAM. ...
Faheem Ali1, Muhammad Amir1, Muhammad Iftikhar Khan1, Muhammad Naeem Arbab1, Abdul Basit2, Muhammad Kashif Khan1, Gulzar Ahmad1
AN SVC BASED POWER QUALITY IMPROVEMENT MODEL FOR CONSUMERS CONNECTED TO WEAK BUSSES IN DISTRIBUTION SYSTEMS
...step-wise reactive power injection which does not meet the changing load requirements. Real time improvement requires constant monitoring and real-time injection of Volt-Ampere-Reactive (VAR) which is possible with the help of a Static VAR Compensator (SVC). An SVC based Power Quality Improvement (PQI) is proposed in this paper which addresses the real time varying reactive VAR requirement with reduced harmoni...
Nashitah Alwaz1, Safdar Raza1*, Sadaqat Ali1
A COMMUNICATION-FREE SIGNAL GENERATING TECHNIQUE FOR REACTIVE POWER CORRECTION IN ISLANDED MICROGRID
...anges in microgrid. This injection disturbs the actual active power sharing which is helpful for the computation of reactive power sharing errors. These errors are minimized by utilizing the proportional integral (PI) controller. The PI controller modifies the y-intercept
and the slop of Q-E (Reactive Power –Voltage) droop characteristic for the correction of reactive power sharing error. Moreover, ...
Shakir Azim1*, Sahar Noor1, Rehman Akhtar1, Qazi Salman Khalid1, Tufail Habib1, Usman Ghani2
SETTING OF PROCESS PARAMETERS AND EFFECT OF MOULD QUALITY ON SURFACE ROUGHNESS OF PHARMACEUTICAL CUP OF POLYPROPYLENE
...quality prerequisites of injection molded items have turned out to be progressively extreme. The setting and optimization of key process parameters are acknowledged as important means to deal with liven up the quality of the molded items. Inappropriate or unseemly settings of parameters normally create quality and production issues. Minute changes of significant molding parameters may give an extensive effect to the plastic product’s ...
Asad Naeem Shah1,2, GE Yun-shan1, HE Chao1,3
MACROSCOPIC SPRAY CHARACTERISTICS OF DIESEL AND BIODIESEL-DIESEL BLEND: AN EXPERIMENTAL COMPARISON
...vestigates the effect of injection duration and ambient pressure on spray development, spray tip penetration (L) and cone angle (β) of biodiesel-diesel (20%/80%: v/v) blend (B20) and diesel, and hence compares the quality of the fuels. Both ‘L’ and ‘β’ play a key role in the fuel evaporation and mixing of fuel with air in the combustion chamber. It was found that the injection...
Magbolah Salem Helal Alzahrani
...reated with subcutaneous injection of CCl4 in paraffin oil 50% V/V (2ml/kg b. wt.) twice a week for 2 weeks to induce chronic liver damage. After injection of CCl4, blood samples were obtained using the retro-orbital method to confirm liver damage and estimate liver function. Rats given CCl4 prior to feeding M. chamomilla demonstrated decrease in serum lipoprotein HDL, LDL, VLDL fracti...
Shakeel Ahmed* and Sarwat Jahan
... normal fed monkeys. NMS injection induced a significant increase (P<0.05) in T and GH concentrations compared to saline treated animals suggesting the possible involvement of GH in NMS induced secretion of testosterone. In summary our results suggest that NMS is a positive modulator of HPG axis and pituitary hormones like GH might be playing an intermediate role.
...
Yujun Zhao and Jianping Fan*
...the effects of Kanglaite injection on apoptosis and expression of apoptosis-related factors Bcl-2 and Bax protein and the activities of Caspase-3 and Caspase-8 in colon cancer SW480 cells were studied. Compared with the control group without medication, the proliferation inhibition rate of cells processed with Kanglaite injection (10, 20 and 40 μL/mL) significantly increased (p <0.05), the apoptosis rate increas...
Tafail Akbar Mughal1, Shaukat Ali1*, Hafiz Muhammad Tahir1, Shumaila Mumtaz1, Samaira Mumtaz1, Ali Hassan1 and Syed Akif Raza Kazmi2
...ced by alloxan. A single injection of Alloxan (150 mg/kg) was applied intravenously to induce diabetic phenotype. Alloxan induction raised the overall level of triglycerides, low density- lipids (LDLs), and cholesterol and reduced the level of high density lipids (HDLs). Oral administration of 200 mg/kg aqueous extract of B. lycium Royleroot bark for 28 days rescued all these changes. This study shows that the root bark extract of B. lycium Royle...
Xuya Zhou1, Ying Liu1, Deqin Xu1, Jie Bao1, Yaru Cao2 and Yong Jin3*
...ipid and intraperitoneal injection of streptozotocin. The adjuvant-induced arthritis model was established by Paraffin/BCG. We used western blot to explore the protein expression levels of COX-2 and cytochrome P450 (CYP) 4A1. The expression of CYP4A1 was detected by immunohistochemistry. Enzyme-linked immunosorbent assay (Elisa) was used to detect the prostaglandin E2 (PGE-2), 20-Hydroxyeicosatetraenoic acid (20-HETE), endothelin 1 (ET-1) and B-type natriureti...
Ahmed Weli Hussein Osman1,2, Rakibul Islam1, Md. Bazlar Rashid1, Abdiaziz Idiris Mohamud2,3* and Pravin Mishra4
Nanoparticle Tactics for the Oral Delivery of Insulin in Alloxan-Induced Hyperglycemia in Rat (Rattus norvegicus domestica) Model
... by administered alloxan injection at dose of 120mg/kg body weight intraperitoneally. Group T0 was kept for negative control while T1 was kept positive control. T2 were considered as insulin treated group at 21IU/kg and T3 as insulin with nano particles of same dose as insulin. Over the sequence of the experimental trials, observations were noted for blood glucose level, glycosylated hemoglobin, and body weight. Blood glucose levels were increased significantl...
Saima Yaqub1, Tahir Yaqub1*, Muhammad Zubair Shabbir2, Asif Nadeem3, Aziz-Ul-Rahman1, Muhammad Furqan Shahid1, Zarfishan Tahir4 and Nadia Mukhtar4
...d in practices of shared injection equipment (21.17%; 95% CI: 25.07-40.21; p<0.0001), injected previously used syringes (30.2%; 95% CI: 23.4-37.99; p=0.0016), sex with IDUs (37.78%; 95% CI: 25.11-52.37; p=0.002) and those with an age between 30-39 years (27.17%; 95% CI: 19.13-37.04; p=0.039). The pol gene-based phylogeny and subtyping classification categorized the under-study sequences representing subtype A (n = 12; 46.1...
Ying Liu1, Shankun Liu1, Hui Wang2 and Weihua Su2,*
...) single intraperitoneal injection was used for the induction of diabetes. The rats were received the various dose of caffeic acid. After the pregnancy (19 day) the blood sample was collected via puncturing the retro arbital and collecting the fetus from all the groups. The rats, fetus weight and placental weight were calculated. The blood glucose level, serum, lipids, antioxidant, cytokines and C-peptide was recorded. GDM group rats showed the lower weight of...
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
...tic feed prior and after injection (G1b, G2b). Provision of probiotics was evident to alleviate pathogen virulence in fish. The emerging multi-drug resistance and related side effects of conventional therapies have caused researchers to explore alternate strategies, such as probiotics employment for disease control in aquaculture.
...
Guangyu Wang, Xu Tian, Lintao Liu, Junyang Liu, Bo Zhang and Jingming Dong*
...eived sodium hyaluronate injection treatment, while the study group was given SJHXT combined with sodium hyaluronate. The therapeutic effects were compared between the two groups. Comparison of Lysholm knee score scale, WOMAC score, and joint VAS score between the two groups showed that the study group had a significant advantage over the reference group after treatment, p<0.05. Comparison of synovial fluid inflammatory factors before and after treatment be...
Ramadan, F. Abbas1;H.M.Salem1; M.H. Belal2, and A. M. M. Abd-Alla3*
Allam A. Megahed1; Hoda M.A. Waziri2; Khaled A. El-Dougdoug3; Badawi A. Othman3; Sirag M. Lashin1; Mohamed D. Hassanin1 and Mahmoud A. Ibrahim4
...inst BtMV and BNYVV by 5 injection doses each, using different injection types obtained from rabbit bleedings 10 days after the last injection had the titer of 1:2048 and 1:1024, respectively by indirect-enzyem linked immunosorbent assay (I-ELISA) technique. The produced antisera were evaluated and compared with foreign antisera using tissue printing immunoassay (TPIA) and dot blot immunoa...
AdlyAbd-Alla1,2, MahaNada3, Francois Cousserans1, and Max Bergoin1
A. A. Rezk1,3, K. A. Alhudaib2 and A. M. Soliman2,3
...and white rabbit using 6 injections at weekly intervals intravenously and subcutaneous. The rabbit blood was collected by bleeding and the antiserum was obtained. IgG was purified and conjugated to alkaline phosphatase. The conjugated antiserum was evaluated by indirect enzyme-linked immunosorbent assay (ELISA). The conjugated antisera that prepared in this study were succeeded to detect the virus in infected plants....
Ola Youssef 1; EL-Deeb A.H.2 Nassif S.A.1 and Ahmed A. El-Sanousi 2.
...y by 10 fold field doses injection in SPF chicks. The challenge test results
revealed 80% protection for A, B and C batches while D and E batches gave 90% protection. The
shedding of challenge virus was significantly low with mean of 2 logs 10 in the vaccinated group
compared with control group.
Conclusion: the developed evaluation protocol could depend mainly on q PCR for identity and
titration of H5...
Hanan Sayed1, Mohamed S. Madkour1, Hussein A. Hussein2, Mounir Mohamed Elsaft 3,
Marwa F. El saied3, Ahmed A El-Sanousi2
...rcial layer chicken, the injection was
repeated as a booster vaccination one month after the first vaccination. Humoral immune response was
measured by HI for both H9N2 and LaSota viruses weekly for one and a half month after the booster
vaccination, then monthly until the end of duration. Challenge trials were carried out after a month
from the first vaccination, and after two and six months from the booster vac...
Hanan Sayed 1, Mohamed S. Madkour 1, Hussein A. Hussein 2, Ahmed A El-Sanousi 2
...rcial layer chicken, the injection was
repeated as a booster vaccination after a month from the first vaccination. Humoral immune response
for IBV was measured by ELISA weekly until about one month after the booster vaccination then
periodically till the end of duration. Challenge trial for both IB strain was repeated after a month from
the first vaccination and after two and six months from the booster vaccinati...
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...identified by testicular injection. The results showed a developmental time specific SCD1 expression in the goat mammary gland tissue and muscular tissue. The rate of positive expression of exogenic gene and exogenic protein in F2 transgenic mice was 30% and 6.67%, respectively, Moreover, muscular fat content in the positive group was significantly higher than the control group both in F1 and F2 (P > 0.05). The rate of positive expression of exogenic...
Chao-Qun Shi1, Hong-Ying Lin1, Jin-Qing Zheng1, Fan Yang1, Kwame Ayisi Lartey1, Dan-Ju Kang1, Hwa-Chian Robert Wang2, Ravi Gooneratne3*and Jin-Jun Chen1*
...>i.p) E. coli injection. Serum IgM, IgA, IgG, TGF-β1 mRNA expression in duodenal villi, and histopathology of duodenum were also studied. TFCO significantly increased the IgA and IgG concentration (P<0.05), reversed the intestinal mucosal damage caused by E. coli, and increased the relative expression of TGF-β1 mRNA. Therefore, TFCO has potential to improve immune function against E. coli infection, restored the int...
Aneela Hameed1, Faqir Muhammad Anjum2, Zia ur Rehman3, Saeed Akhtar1, Asim Faraz4*, Majid Hussain1 and Amir Ismail1
...bjected to intramuscular injection of oxytocin (20 IU) and other was kept as control. Significant variations were obtained in milk composition along with lactations stages. Decrease in fat, protein, lactose, solids not fat and total solids contents and increase in ash contents were noted in oxytocin administreted milk. Minerals’ analysis of the milk samples were conducted and it was found that lactation stages have significant effect on minerals composit...
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
...gs were administered LPS injection with or without continuous adrenocorticotropine hormone (ACTH) pretreatment. Twenty-four 30-day-old Duroc × Landrace × Large White crossbred piglets (12 ± 0.5 kg) were randomly assigned to 4 treatments: control, LPS, ACTH, and ACTH+LPS groups. Each group consisted of 6 male piglets. All ACTH pigs and ACTH+LPS pigs were injected intramuscularly with ACTH (2.25 IU/kg body weight) for 7 consecutive
Abdullah Abdo Albegali1, Tayyaba Aftab1, Atiq-ur-Rehman1*, Amir Rashid1 and Mayada Mohammad1,2
...red with intraperitoneal injection of Triton X-100 to induce hyperlipidaemia. Group B was administered with distilled water and high cholesterol diet on daily basis during the whole treatment and served as hyperlipidaemia control group. Group C was kept as a standard group and received simvastatin (10 mg/kg), while group D received a methanol extract of the plant (800 mg/kg). Lipid profile was analyzed using different kits of Roche Cobas C111 chemistry analyze...
Muhammad1, Saadat Mehmood1,2, Atif Muhammad1*, Zaib Ullah3
...the occasion of surgery, injection, and blood transfusion. Furthermore, awareness campaigns regarding blood transmission and health hazards of Hepatitis should be launched for the population.
Novelty Statement | The study indicates first observations of Hepatitis B and C infections from District Swabi. It represented that the rate of disease is quite high in the district and proper control is re...
Mohammad Hussain Haidary1, Rozanaliza Radzi1*, Muhammad Waseem Aslam1, Seng Fong Lau1, Farina Mustaffa Kamal2, Ahmad Rasul Radzali1
...ralesional triamcinolone injection was able to manage FCGS in the short term. Serratiopeptidase or papain alone was not effective in the management of the FCGS.
Keywords | Feline chronic gingivostomatitis, FCGS, Caudal stomatitis, Halitosis, Dental extraction
...
Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*
...g BW via intraperitoneal injection, respectively. In both trials, the blood glucose concentration was determined at 0, 10, 20, 40, 60, 120, and 180 min through small brachial blood vessels. As a result, the blood glucose of both group was increased immediately after being treated with glucose and reached peaks at 20 min, then recovered to the normal at 60 min. Birds treated with glucose had greater blood glucose concentration and area under the curve (AUC) in ...
Doaa Sh. Mohamed1, Nema S. Shaban2 , Mai M. Labib3, Olfat Shehata4
...prior to the doxorubicin injection and two weeks along with doxorubicin injection. The effects and mechanisms of doxorubicin on superoxide dismutase activities were elucidated by molecular docking studies. Treatment with almond oil attenuated lipid peroxidation, improved superoxide dismutase (SOD) activities that are associated with doxorubicin administration. Also almond oil considerably modulated the gene expression of tol...
Arman Sayuti1, Hamdan Hamdan2, Miftahul Jannah3, Safriadi Safriadi3, Teuku Nasri3, Tongku Nizwan Siregar2*
...mL each. After the third injection, rabbits of K1 and K2 groups were mated with male local rabbits. For measurement of steroid hormones concentration using an enzyme-linked immunosorbent assay (ELISA), blood samples were collected on day 0 (the day when rabbits mated) for estrogen measurement and on day 7 and day 14 for progesterone measurement. Furthermore, pregnancy examination using ultrasound was carried out on the 19th day after rabbits were mated. The av...
Khaled El-Kholy1*, Yaser Rizk2, Mohamed El-Helbawy1, Eman El-Said1
...were the control without injection (T1), and those in 2nd and 3rd groups were injected with clove oil (25.0 “T2” and 12.5 “T3” µml/ rabbit/week), respectively. whereas, 4th and 5th groups were injected with Salbutamol (50 “T4” and 25 “T5” µml/rabbit/week). The significant (P<0.05) increased in growth performance was observed in rabbits injected with low level of clove oil (T3) compared with other g...
Amal Hamad1, Ashraf M. Abu-Seida2*, Faisal A. Torad2, Nahed S. Thabet3, Shabaan M. Gadallah1
...d blood values following injection of Nalbuphine HCl, Fentanyl citrate, Tramadol HCl and Meloxicam in dogs. Forty clinically healthy mongrel dogs were randomly divided into 4 groups (10 dogs each) according to the analgesic agent used. Doses of 0.5mg/kg Nalbuphine HCl, 5μg/kg Fentanyl citrate, 2mg/kg Tramadol HCl and 0.2mg/kg Meloxicam were given IV in groups A, B, C and D, respectively. The zero time for evaluation was 10 min for Nalbuphine HCl, 45 min for...
Fan Xu1,2*, Cheng Wu3 and Su Zeng1
...ism was easily caused by injection or small pot dripping, which may be related to the insufficient amount of solvent and the incomplete dispersion of lipid microspheres. As for the two dosage forms of lipo-PGE1 and DN-PGE1, the average particle size and PFAT5 after the preparation of DN-PGE1met the USP standard, and the stability indexes were more advantageous. The clinical treatment of alprostadil targeted injection can be ...
Wael Felefel1*, Mohamed EL-Beskawy2, M. F. El -Dakroury3, Mohamed Morsi Elkamshishi4, Eman Sayed Mohammed5
...ed to that of Doramectin injection on naturally infested dogs with hard ticks. Forty five stray dogs with age above 6 months were collected from Alexandria governorate with heavy or moderate ticks infestation. The captured dogs were divided into 3 groups, each group includes 15 dogs, and categorized as follows; group (1): (Positive control group), group (2): (Doramectin injected group), and group (3): (topical Fipronil group). It was observed that the overall ...
Qiong Xiang1, Jing-Jing Li1, Qian Zhang1,3, Rong-Bo Tian1 and Xian-Hui Li1,2*
...cause about 15 min after injection the significant up-regulated expression of the SSTR2 proteins are found in inflammatory DRG neurons compared to that of in control DRG neurons. The process is also time-dependent, because no difference is seen after one-day injection. Meanwhile, we also analysed the SSTR2 expression in other types of painful models (i.e., DR and SNI); Thus, our data suggested that changes of SSTR2 expressio...
Zhongqing Wang1, Qiuyue Wu1, Ruiling Ye1, Fazul Nabi1,4, Yangfei Shang1, Sarfaraz Ali4 and Juan Liu1,2,3*
...(5×107CFU) via i.p injection (0.5 mL/time/d) after seven days of pre-administration except in CG. After the induction of the diarrhea, the pathological signs and the quantity of iIELs in small intestine and the mRNA level of IL-6 and TNF-α in duodenum were analyzed by qRT-PCR from all groups at 6 h and 4 d, respectively. IG group shows abnormal mental state and the lack of the appetite; however, AMP pre-treatment caused the normal state and appetit...
Zhaojun Wang1, Yang Zhou2 and Xinghua Song3*
... observe the efficacy of injection of sodium hyaluronate combined with betamethasone into knuckle articular cavity in knee osteoarthritis treatment, and to analyze its effect on the prognosis of knee joint function. A total of 120 knee osteoarthritis patients admitted to our hospital from January 2019 to February 2020 were selected as the study subjects and randomly divided into control group and observation group (60 cases each). The control group was given <...
Lukman Affandhy*, Muchamad Luthfi*, Yeni Widyaningrum, Dian Ratnawati, Mutia Primananda
...ce by intramuscular (IM) injection. Semen collection was carried out for 16 weeks; divided into two periods, namely 8 and 16 weeks after GnRH administration. Parameters that were measured include volume, acidity, color, consistency, mass motility, individual motility, spermatozoa concentration, viability, and libido. Data were analyzed using the ANOVA test. The results showed that the volume and pH of semen did not differ in all treatments, but treatment A sho...
Heba S.S. Salem1, Hend M. Megahed2, Marwa M. Sarhan2, Maha M. El Alem3, Gehan N. Alagmy3*
... received a subcutaneous injection of bee venom (BV), the fourth group was injected intra-articulately with S. aureus and treated with a subcutaneous injection of BV, the fifth group was injected intra-articulately with S. aureus and treated with a subcutaneous injection of BV and gentamicin. On the 7th and 14th days, post-infection animals were examined for hematological, biochemical, and...
Dhiya Altememy1, Mohammad Darvishi2*, Samira Shokri3, Saber Abbaszadeh4
...betic by intraperitoneal injection of alloxan. After the rats were anesthetized, 2cm2 wounds were created and inoculated with bacterial suspension of S. aureus (ATCC 12600) equivalent to the 0.5 MacFarland tube. The hydroalcoholic extracts of Origanum vulgare, Hypericum perforatum, and their active ingredients including carvacrol and hypericin, were prepared, and then TiO2N.Ps. were prepared from them using isopropoxide. The antibiotic methicillin and calcium ...
Abhishek Pandit1, Suman Poudel2, Manish Gautam3* and Shambhu Shah4
...ction, number of hormone injections, number of cattle handled, and injection time. Increased labor and upfront cost of hormone treatment, standard degree of supervision, and decent handling facilities are some of the drawbacks of this technique. Successful estrus synchronization necessitates optimum nutrition, a good body condition score, the best semen quality, general health, and an efficient estrus detection technique.
Jiahua Peng1,2, Shiyao Hua3* and Qian Wu4
...kg/d). DHEA subcutaneous injection was used to construct the PCOS model of the polycystic ovary syndrome (PCOS) model of rats. The general condition of rats (body weight, ovary weight) and ovarian tissue pathology changes were observed. The changes in content of testosterone (T), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were detected. The fasting blood glucose, serum insulin and insulin sensitive index changes were analyzed. The RT-PCR m...
Md. Aminul Islam1*, SM Mostafizur Rahaman Sumon1, ANM Aminoor Rahman2, Fahima Khatun3
...stant against Ivermectin injection (100%) in all the investigated dairy farms. These findings are crucial for the selection of anthelmintics for the treatment of various types of nematodes in dairy cows and might be crucial in preventing anthelmintic resistance in dairy cows.
Keywords | Anthelmintic resistance, Dairy cows, Effectiveness, Helminth infections, Local anthelmintics
...
Eighty Mardiyan Kurniawati1*, Budi Santoso1, Fedik Abdul Rantam2, Widjiati3, Budi Iman Santoso4, Tri Hastono Setyo Hadi1, Gatut Hardianto1, Hari Paraton1
...l seeding, the stem cell injection group, and the control group. There was a significant difference between groups only in angiogenesis at day 21. Conclusion: Freeze-dried amnion plays a role in the wound healing process, especially in angiogenesis in the vesicovaginal fistula repair model. The use of freeze-dried amnion can be applied to assist the wound healing process in patients but still needs to be studied in humans.
Keywords | Freeze-dried amnio...
Shakeel Ahmed Wagan1, Akeel Ahmed Memon1, Qudratullah Kalwar2*, Asmatullah kaka1, Mohammad Farooque Hassan2, Hidayatulah Soomro2, Safia Kadhro3, Abdullah Sethar4 and Muhammad Jehangeer5
...C). In group A, 02ml I/M injection of GnRH (25µg lecirelin acetate) were injected on day 0 and 9th of the treatment. On 7th day 05ml I/M injection of PGF2α analogue (5mg/ml dinoprost tromethamine) was administered. The group B animals received same treatment but Controlled Internal Drug Releasing Device (CIDR) (containing 1.38mg progesterone) inserted into vagina on 0 day of the treatment and removed on 7th day o...
Nadia A Eltablawy1, Ibrahim El Tantawy El Sayed2, Hamed Mohamed Abdel Barry2, Marwa A. Ibrahim3*, Maha Nageib Ahmed Serag ElDein2
... 2.5 mg/kg of DOX in six injections over 2 weeks), TME was solely administered for four weeks, two weeks pre and two weeks co-administration of TME (100 mg/kg) with DOX as well as TME (100 mg/kg) and TME (200 mg /kg) were administered after two weeks of DOX injection. Results: A relatively high TPC and TFC with considerable antioxidant capacity as evaluated by DPPH, FRAP, and FRAC examinations were recorded. DOX significant...
Shah Murad Khan1, Hamayun Khan1, Younas Muhammad1, Haq Aman Ullah1*, Muhammad Tariq Tunio2, Ali Gohar2 and Fazli Rabbani3
...stered with PGF2-α injections on day -38, -24 and -3. GnRH-1 was given on day -10 and 0, along with timed artificial insemination after 16 hrs. Animals in MSS group were administered with a controlled internal drug release (CIDR) insert on day -7 with simultaneous use of prostaglandin 2-alpha (PGF2-α), GnRH-1 at day -5, removal of CIDR at day 0, concurrent with two doses of PGF2-α at 8 hr. interval, GnRH-2 on day 3, followed by TAI after 16 h...
Ferzana Bhatti1, Fiaz Hussain2, Zain ul Abdin1*, Muhammad Arshad1, Saqi Kosar Abbas2 and Muhammad Zeeshan Shabbir3
...tify;">The role of venom injection by an ectoparasitoid, Bracon hebetor (Say) (Hymenoptera: Braconidae) in inducing paralysis and developmental arrest in its host, Galleria mellonella (L.) (Pyralidae: Lepidoptera) was studied. Bioassays were performed with microinjections carrying crude and diluted venom and secretion of Dufour’s gland of B. hebetor into healthy and mature host larvae. To observe the effects of venom <...
USMAN AHMAD*1, AMTUL JAMIL SAMI1, MADEEHA KARAMAT1, MADEEHA KHALID1, FAIZAN NAEEM2 & MUHAMMAD JAMIL YOUSAF3
...t rid of the traditional injection treatment which is a painful process and can cause infections and allergy, the drug delivery through the skin cells can substitute only if the skins cells permeability for the drug is increased, which is now done by chemical, ultrasonic and electrical methods. Drug delivery through the largest organ (skin) by dermal patches of psyllium based hydrogels was observed. In this article we have fabricated different psyllium based d...
Zubair Ali, Muhammad Sohail*, Yasir Ameen, Hamidullah, Ishtiaq Ahmed and Mehwish Malik
...triaxone 4gm Intravenous injections daily for three days along with (penicilin-streptomycin combination) 10 gram intrauterine. Pyometra and Hydrometra were first treated with Cyclomate to regress the Corpus luteum. In repeat breeding issues due to nymphomenia, follicular cysts, Luteal cysts, Buserelin 0.0105 mg/lecirelin 50µg and PGF2α Analogue100µg Intramuscular (IM) were used. Further research on the efficacy of protocols that integra...
Anhar Ibrahim Elhanafy*, Amr Mohamed Mousa, Amaal Mohamed Kamal
...as control. Twice weekly injections of does were carried out along 12-weeks. Results revealed that BV treated does were significantly improved RBCs count, WBCs, serum total protein, albumin and globulin concentrations, as compared to control. Upon treatment, a significant decline in aspartate aminotransferase, alanine aminotransferase, serum total bilirubin, direct bilirubin and in direct bilirubin, serum creatinine, urea content, serum total cholesterol, tr...
Oussama Zghari*, Mouloud Lamtai, Sofia Azirar, Mohamed Yassine El-Brouzi, Hajar Benmhammed, Aboubaker El-Hessni, Ali Ouichou, Abdelhalem Mesfioui
... single intrahippocampal injection of NaCl, MEL or AlCl3. Before two weeks of intrahippocampal surgery period, MEL (4 mg/kg) was intraperitoneally injected (Group II and Group IV). Thereafter, control group (group I) received 2 µl NaCl (0.9%) and groups III and IV received 2 µl AlCl3 (2 mg/kg) intracerebrally into the right ventral hippocampus. Five days after treatment period, all the rats were subjected to the neurobehavioral tests. The animals w...
Hala A. Amin1; A. Barakat2; A.A. Abou Zeid1
...ained from rabbits after injection with the BBP-rCTV-CP fusion protein showed comparable reactivity in the serological detection of CTV. This antiserum could be used at dilution or 1: 10.000 at the detection stage in an indirect ELISA (IDAS-ELISA) and was efficient for trapping the virus in standard ELISA. These polyclonal antibodies reacted with a wide range of CTV isolates from different Egyptian geographic sources, and or different biological properties.
Waheed Khan1, Muhammad Ajmal Khan2, Bakhtawar Khan1*, Aftab Amin3, Muhsin Ali4, Awais Farid, Ali Hazrat5 and Muhammad Yahya5
...given an intraperitoneal injection of alloxan (150 mg kg-1, b.w.) to cause diabetes. There were five groups of mice (n=10): group 1 (normal control) received normal food and water, group 2 (diabetic control) received normal food and clean water, group 3 (diabetic mice) received 200 mg of methanolic plant extract, group 4 (diabetic mice) received 400 mg of methanolic extract, and group 5 (diabetic mice) received 10 mg of the medication Glibenclamide for 28 days...
Atena Azarniush1, Majid Moghbeli2*, Farshid Kafilzadeh1, Mohammad Kargar1 and Hooshang Jamali1
...m prone to bleeding. PRP injection therapy is a therapeutic method in use to relieve this condition. It may lead to infections due to the injection, as well as platelet resistance. Therefore, an attempt was made to find an alternative treatment, and the recombinant Interleukin 11 (IL-11). In this study, IL-11 gene owning two restriction enzyme sites of BamHI and XbaI with a final size of 609 bp was synthesized by GenRay (Chi...
Indira Jurinskaya1*, Tynyshtyk Kenzhebaeva2, Kairat Iskakov3, Bekzat Niyazbekov1
... underwent intramuscular injection of the cyclophosphamide drug at a dose of 7 ml/kg of body weight. The results showed one-year-old goats of the Kazakh coarse-haired and Gorno-Altai downy breeds subjected to an intramuscular injection did not show a molt of down, but the output during their brushing was, respectively, 14.3 and 7.8% higher than that of the peers in control groups due to better separation of down. The down ou...
Aamir Ali, Hafiz Muhammad Tahir*, Azizullah, Shaukat Ali, Muhammad Summer, Ali Haidar Gormani, Saira Nawaz and Ayesha Muzamil
...s induced by carrageenan injection while formalin was injected to mice paw for induction of pain. These extracts significantly decreased edema in mice paw. The level of TLC and RBCs was increased to normal. Platelets level was also increased in mice treated with mulberry leaves extracts. Furthermore, these extracts significantly reduced the inflammatory pain and licking response triggered by injection of formalin in mice paw...
Abdulaziz A. Alaqil
...ainst phytohemagglutinin injection, 44% in the anti-sheep red blood cells antibody titer, and more than 50% in T- and B-lymphocyte stimulation index, while recording a double increase in the heterophil to lymphocyte ratio. In contrast, the productive traits and immunological aspects were significantly (p < 0.05) augmented by PR supplementation to the broiler diets. Furthermore, PR supplementation successfully restored the broiler production and immune respo...
Zahra Abdulameer Al-Zayadi1, Hana Kadum Shanan1, Al Salihi Karima Akool2*
...duced by intraperitoneal injection of Polyvinyl pyrrolidone K-30 (PVP) in mice. 72 mice divided randomly into eight groups (G1, control group; G2, Sc-RE group; G3, chemotherapy alone; G4 cancer- group without treatment, G5, cancer-induced + chemotherapy; G6, cancer induced+ Sc-RE/ low dose; G7, cancer induced+ Sc-RE/ moderate dose; G8, cancer induced+ Sc-RE/ high dose). Liver and kidney samples were collected from all mice during different experiment periods, ...
Abdulaziz A. Alaqil*
...induced by intravenously injection of lipopolysaccharides (LPS). A total of one hundred twenty, 40-week-old, laying hens from the Hy-line breed were allocated at random into four groups of 30 hens each. For eight consecutive weeks (40–48 weeks of age), two groups of the layers were given a basal diet as control, while the remaining two groups were given a basal diet enhanced with a 2 g/kg diet of LA bacteria. At 47 wk of age, layers in one of the co...
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
...condition at the site of injection and return of skin to normal color and thickness. Doxycycline+ colistin combination helps in reduction of lesions in treat infected birds, with marked improvement in measured parameters. We recommended active actions to prevent causes and factors helping in including cellulitis, regular lowering bird density, enhancing restrict biosecurity, modulating the vaccination timing, improving management practices, as well as applicat...
Tahir Rasool Qamar1, Sanaullah Iqbal1*, Muhammad Nasir1 and Habib-ur- Rehman2
...ministering carcinogenic injections. After one-month, groups G2-G4 received four subcutaneous injections of carcinogen 1,2-dimethylhydrazine dihydrochloride (DMH) at dose of 40 mg/kg body weight, twice a week for 2 weeks. After DHM injections, chicory powder treatments were started to group G3 as well and group G2 was DHM control group receiving only basal diet while G1 was normal control ...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
... results showed that BPA injection led to an obvious increase in the activities of liver enzymes, elevation of hepatic oxidative stress (a significant increase in lipid peroxidation concomitant with a significant decrease in glutathione content and antioxidant enzyme activities), tumor necrotic factor-α (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage...
A.M. Abdul Azeem1, A.N. El shahat1* and Mohamed H.M. Abd el Megid2
... results showed that BPA injection led to an obvious increase in the activities of liver enzymes, elevation of hepatic oxidative stress (a significant increase in lipid peroxidation concomitant with a significant decrease in glutathione content and antioxidant enzyme activities), tumor necrotic factor-α (TNF-α) and apoptotic marker Caspase-3 compared to the control group. Rats received BPA along with SeNPs-CP showed significantly less severe damage...
Xiaowei Huang, Yajie Wang, Jia Zhou, Junxiu Liu, Zhe Lin and Ruili Li*
...model was established by injection of cyclophosphamide (CTX) intraperitoneally. 60 SD rats were randomly divided into 6 groups, control, model, positive drug (compound E-jiao slurry), SBG high-, medium- and low-dose (SBG-H, SBG-M, SBG-L) groups. After 21 days of intervention, the changes of thymus and spleen indexs were compared, the levels of white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), hematocrit (HCT) and platelet count (PLT) in peripheral...
Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2*
...1mg/kg) com2 groups all injection 0.1 ml in 1 x 109 CFU E coli 0157 :H7 by urinary catheterization route excepting in negative control and all groups excepting negative groups resulted significant increase(P< 0.05) in white blood cells, monocyte, and neutrophils in blood after three days comparison with negative control and in all experimental period (14 days) which was increase significant (P< 0.05) positive control (P.C.). However,(DC,COM1,COM2) grou...
Rimsha Ijaz, Rida Zahra, Naseer Ali Shah*, Haroon Ahmed, Nazish Bostan and Sadia Sattar
... instruments, and unsafe injections. Globally a total of about 170 million people have been afflicted with this malady per year and affects approximately (6%) of Pakistan’s population. HCV is RNA virus and there are seven primary genotypes and 67 subtypes in it. Determination of genotype is important for treatment and medication response. This study aimed to calculate the frequency of HCV infection and pattern of genotype distribution in suspected indivi...
MUHAMMAD ASLAM*, ASAD GULZAR, AMINA ASGHAR, MISBAH IRSHAD, ZAHRA NOREEN, SADAF JAVAID, ABRAR HUSSAIN, UMAIR AHMAD & SAMREEN KOUSAR
...ation of levofloxacin in injection form. The λmax for
levofloxacin was found to be 293nm using 0.1N HCl as a blank.
Calibration curve data proved that the proposed method was linear in
the concentration range of 3 to 7μg/mL. The proposed method was
validated to determine linearity, precision, accuracy, ruggedness and
robustness. The results of above analytical pa...
Ali Mosa Rashid Al-Yasari1, Zahid I. Mohammed2, Haider S. Almnehlawi3,4, Ali F Bargooth5, Nawar Jasim Alsalih1, Huda F. Hasan6*, Mohenned A Alsaadawi1
... group received one (ip) injection of streptozotocin (40 mg/kg), and the STZ-diabetes+Camels’ Milk group received a STZ injection (40 mg/kg) along with daily (ad libitum) bottled camel milk for 28 days. Zero time, one day, one week, two weeks, three weeks, and day 28 following STZ injection were used to monitor fasting blood glucose, insulin, and glucagon. Animals were sacrificed on ...
Wenxiao Jia1*, Yilinuer Yilihamu1, Yunling Wang1, Shuang Ding1, Dilinuerkezi Aihemaiti1, Hanjiaerbieke Kukun1 and Yanhui Ning2
...was completed. Following injection, the animals were fed a high-fat diet for 16 weeks. High-resolution magnetic resonance imaging (HR-MRI) and a histology-based method were used to examine the abdominal aortic lesions. The overall mortality rate of the New Zealand rabbit model of unstable atherosclerotic plaque was 17%. The MRIs indicated that the abdominal aorta in the experimental group was eccentrically thickened, while the vascular wall of the abdominal ao...
Frah R Kbyeh1*, Ahmed N. Abedsalih2
... groups(8/each) groups injection 0.1 ml in 1 x 109 CFU Escherichia coli 0157: H7 by urinary catheterization route excepting in negative control and all groups resulted in a significant increase in urea creatinine, sodium, Potassium, chloride concentration in the blood after three days comparison with negative control and in all experimental period (14 days) which was no significant change (P < 0.05) between positive control (PC) and diclofenac treated gr...
Rana Mahmood Ahmad*, Orooba M.S. Ibrahim
... Freund’s adjuvant injection with SaZnONPs and piroxicam at doses 2.5 and 5 mg/kg B.W., respectively, while (Group 1) was positive control. Blood samples were obtained using cardiac puncture at three time points during the experiment: at the beginning (zero time) and on the 12th and 21st day. These samples were collected specifically for serum collection. In the present investigation, the administration of SaZnONPs to infected rats yielded noteworthy red...
Usama Mahalel1, Barakat M. Alrashdi1, Ibrahim Abdel-Farid1, Sabry El-Naggar2, Mohamed Hassan3, Hassan Elgebaly1 and Diaa Massoud1,4*
...results showed that CCl4 injection significantly reduced the body weights of mice and increased the relative liver weight. Treatment with M. forsskalii extract after CCl4 injection restores the weights of mice to the normal range and reduced the relative weight of the liver. Mice that were injected with CCl4 exhibited elevation in the liver enzymes; aspartate aminotransferase (AST) and alanine aminotransferase (ALT), reducti...
Madbouly*, H.M.; Saif**, M.A. and Hussein* , A.S.
...at one day of age by SIC injection. Serum samples at 10, 14, 17, 21, 28, 35, and 42 of age for HI test against NDV. Heparinized blood samples at 10, 14, 17, 21, days of age for phagocytic activity of macrophages. All groups were challenged with vvNDV for detecting the protection percent. From this study it could be concluded that MDV vaccine has an immunosuppressive effect on chicks and this could be antagonized by immunostimulant as curcuma longa The surprisi...
Khatab, Eman A.H.; Zein, Salwa N. and Ahmed, Amal A.
...ree different methods of injections. The titer Ofthe prepared antiserum as determined by indirect ELISA with dilution of tissues 1/5 was 1/1024. Purification of the immunogamaglobulin G and conjugate of IgG with alkaline phosphatase were carried out to be used for ELISA detection. The concentrations of IgG and IgG conjugated with alkaline phosphatase were 1.0 mg/ml and 1:1000 respectively. Antiserum produced specific to BBTMV was used for virus detection by us...
Estabraq Hayder Khayoon, Ammar Ahmed Abdulwahid*
...n given an intramuscular injection of ketamine and xylazine to induce anesthesia. The findings revealed the exposure of Acrylamide to rats significantly reduced their tendency to gain weight, a marked rise in the concentration of malondialdehyde and decrease levels of Total antioxidant in blood serum and behavioral differences like Acrylamide greatly decreased the struggling and ascending time in the swimming test, dramatically lengthens the time needed to...
Sena’a M. Hussein1,2, Raffal A. Omar3*
...and repeated lumbosacral injection of liposome carrying lidocaine in comparison with lidocaine hydrochloride. A total of 24 male local breed dogs were divided randomly into the lidocaine group that received 4.5 mg/Kg of 2% lidocaine and liposome group which received the same dose of lidocaine carried by liposome. Pinprick and spinal reflex tests were evaluated each minute for the first five minutes following lumbosacral injection
Budianto Panjaitan1, Husnurrizal Husnurrizal2, V.Y. Edward3, D.A. Nishcaya3, Teuku Armansyah4, Tongku Nizwan Siregar2, Mulyadi Adam5, Arman Sayuti1, Amalia Sutriana4, Hafizuddin Hafizuddin2*
...ext-align: justify;">The injection of prostaglandin F2 alpha (PGF2α) has been adopted as an alternative to improve sperm quality. The difference in sample collection time after administration is assumed to have a positive effect on testosterone concentration. This research aimed to determine the effect of PGF2α injection and the optimal time for sample collection on improving semen quality and testosterone concen...
Xiangmei Chen, Burie Bao* and Mo Degema
...ned with intraperitoneal injection of STZ dissolved in a citric acid-sodium citrate buffer was used to establish a rat model of hyperglycaemia. Metformin hydrochloride tablets were used as a positive control to detect fasting blood glucose. The contents of glycated haemoglobin (HbALc), insulin (INS) and glucose (GLUC3) as well as the protein expression and mRNA levels of sodium-glucose co-transporter 2 (SGLT2) and glucose transporter 2 (GLUT2) were used to stu...
Qamer J. Jadoaa, Raffal A. Omar*
...e effect of intraosseous injection rout of 2% lidocaine hydrochloride and 3.75% Diclofenac, on bone regeneration. Forty-five adult male rabbits of the local breed were employed to create a 3.5mm hole defect in the proximal third of the medial aspect of the tibia by an electric drill with dropping isotonic normal saline to prevent thermal necrosis. The experimental animals were divided randomly into three equal groups, each group include fifteen rabbits (n=15)....
Lijuan Liu1, Jinghang Chen2*, Min Wang3 and Wei Li4
... methods such as oral or injection, inhaled medications are directly administered to the respiratory tract to exert a therapeutic impact, which has clear benefits in the treatment of respiratory illnesses. However, existing inhaled medications are difficult to fulfill clinical prescription demands, and the research and development of novel inhaled pharmaceuticals has significant hurdles due to a lack of comprehensive theoretical and practical experience guidan...
Muhammad Yusuf1*, Abd Latief Toleng1, Sahiruddin1, Masturi1, Hasrin1, Musdalifa Mansur2, Hendrikus Fatem3, Johan Koibur4
...nterval from PGF2α injection to onset of estrus was 42 and 43 h (P>0.05) in Heatsynch and Select-synch protocols, respectively. However, the mean duration of estrus in dairy heifers synchronized using Heatsynch protocol was 25.1 h, significantly higher (P=0.029) than the mean duration of estrus (19.5 h) in Select Synch protocol. It can be concluded that dairy Holstein Friesian heifers synchronized with both the Heatsynch and Select Synch protocols had...
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
...ts after intraperitoneal injection of tetrodotoxin (TTX) on liver of male albino rats. At the age of 8–9 weeks and weighing 190–200 gm, 60 male albino rats were separated into 3 groups: First group of 20 rats received distilled water and regular pellets. Second group of 20 rats received weekly injections of 0.5 µg/kg b.w. by I/P route, which was equivalent to 1/20 LD50 of tetrodotoxin. Third group of 20 rat...
Abdelghafour El-Hamzaoui*, Mouloud Lamtai, Laila Ibouzine‑Dine, Sofia Azirar, Mohamed Yassine El-Brouzi, Ayoub Rezqaoui, Aboubaker El-Hessni, Abdelhalem Mesfioui
...ering daily subcutaneous injections of four different doses 25, 50, 75, and 100 mg/kg of GBH while a control group received 0.9% NaCl. Based on behavioral tests (the open field, elevated cross maze, and forced swim), behavioral alterations were detected, specifically anxiety levels and depressive behavior. Simultaneously, hippocampal oxidative stress markers (catalase, nitric oxide, and lipid peroxidation) were assessed to elucidate the involvement of oxidativ...
Jinzhao Li1, Yawen Zhang2, Binghan Jia1, Yuqiong Zhao1, Huijuan Luo1, Xiaojie Ren1, Yuan Li3, Xiaoyan Bai1, Jing Ye3 and Junping Li1*
...by intraperitoneal (i.p) injection of loperamide hydrochloride (Lop; 5mg/kg), and was evaluated by examining the fecal properties, intestinal propulsion mobility, immunohistochemistry (IHC), western blot (WB) and ELISA for detecting levels of Tau, P-Ser396-tau, P-Ser404-tau, protein kinase C (PKC), p38 MAPK (p38), p-p38 MAPK (p-p38) and µ-opioid receptors (MORs) in the colonic samples. The gene sequencing approach was used to detect alternatively spliced...
Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*
...1st, 28th, 35th day post-injection, respectively, after extracting the total DNA from fresh tissues of immunized chickens, the 3-1E gene was amplified by PCR, the serum IgG antibody was detected by routine ELISA, peripheral blood lymphocyte proliferation response was evaluated by cell proliferation assay. The results showed that a target band of 583 bp was obtained by PCR in DNA vaccine PLGA microspheres group at 7th, 14th, 21st, 28th, 35th day post-
Eman Ali Hussein1*, Majdy Faisal Majeed2
...f a single intramuscular injection of (SZ) at a dose of 50 mg/kg body weight, In contrast, rats in groups 3 and 4 were given oral gavages of TFG herbal extracts (0.15 g/kg BW) in addition to being kept on an identical experimental protocol to that of the rats in groups 1 and 2, respectively. While group 5’s diabetic rats that received orally gavages 3.5 mg/kg of glucophage XR treatment served as positive controls. Treatment with TFG of groups 3 and 4 rec...
Majeed Ajafar1, Hashim Hadi Al-Jebory1*, Mohammed Khalil Ibrahim Al-Saeedi2
...f Lysophospholipid (LPL) injection on hatching eggs on the quality of hatched chicks. A total of nine hundred eggs were used in this study. All the eggs were set in an automatic incubator at the same time. On the 12th day of embryonic age, half of the eggs (450 eggs) were divided into 6 groups (75 eggs per group). The first egg group was kept in the incubator without treatment and was a negative control (NC). The second egg group was injected with 0.3 ml of NA...
Sony Eka Nugraha1*, Marianne Marianne2, Rony Abdi Syahputra2, Aji Najihudin3, Nikmatul Ikhrom Eka Jayani4, Shaum Shiyan5, Nabila Nabila6,7
...ablished by subcutaneous injection of heparin at 2000 IU/kg body weight (BW) for 10 days. After induction, rats were administered C. papaya leaves ethanol extract at various concentrations (50, 100, and 200 mg/kg BW) for 20 days. On day 21, the duke method was used to examine the bleeding time, and the blood clotting time was evaluated using the Lee White method. Blood samples were evaluated for platelet concentration, thrombopoietin concentration, prothrombin...
Nada Hamzah Shareef Al Shabbani1, Marwa Jamal Hussain Al Kinani2*, Tmara Qais Al-Mohammadi1
...on of corticosteroids or injection of corticosteroids to nodules but the response to treatment is less than clent and relapses are very frequent. The objective of this study was to illuminate which of these features prevail and which ones fade in scarred sites which undergo postscabietic nodule development. The time span between August 2021 to May 2022 was allowed to collect samples from patients with (1-60) years old and full of scabies burrows who visit teac...
Sajid Hussain Shah1, Akeel Ahmed Memon1*, Asmatullah Kaka1,
Ahmed Nawaz Tunio2, Abdullah Channo1, Qudratullah Kalwar4,
Muhammad Ibrahim Panhwar3, Kashif Ali Malak1 and Baban Ali Rahoo1
...d control (intramuscular injection of normal saline as placebo on day 0, 7 and 9). Animals of all group underwent fixed time insemination at 16 h of last treatment. Results showed that estrus response vary significantly among the groups (P<0.05). Overall estrus response was observed significantly higher in group B (80%), followed by A (60%) and C (10%). Intensity of estrus was determined by observing the estrus signs. Results showed significantly higher int...
Salman K.A.A1, Mohammed Khalil Ibrahim Al-Saeedi2, Hashim Hadi Al-Jebory3*
...as follows: T1: a 0.3 ml injection of NaCl, the T2, T3, and T4 treatments egg was treated with a 60, 80, and 100 ppm zinc-methionine solution, respectively. After that, 15 chicks from each treatment were selected and given a 48-hour fast. The results were: At six hours, the cholesterol levels dropped dramatically (P≤0.05) in T3 and T4 compared to T1 and T2. Triglyceride concentrations for T3 were significantly lower at 6 hours than for T1 across all exposur...
Hafizuddin1, Sri Wahyuni2, Tongku Nizwan Siregar1*, Amalia Sutriana3, Ririn Triyuliani4, Siti Hanyyah Herviani4, Muhammad Farhan Syuhada4, Nur Salimah4, Muharni Mardiah Eriswandi4, Ratu Permata Ayu4, Putroe Hayatun Nufus4
...s 28 mg. Intramuscularly injections in both groups were performed 5 times with a 12-hour interval. The administration of NaCl in R1 and BPE in R3 was 1; 0.5+0.5; 0.3+0.3 mL, respectively while the injection of FSH in R2 was 4; 4+4; 8+8 mg. Subsequently, rabbits in R2 and R3 were injected with 100 IU hCG and mated with males, while those in R1 were mated without hCG administration 24 hours after the last FSH and BPE treatment...
Husnurrizal Husnurrizal1,2, Hafizuddin Hafizuddin2*, Sri Wahyuni3, Cut Nila Thasmi2, Tongku Nizwan Siregar2,4
...utes after a 0.5 ml NaCL injection. In groups P1, P2, P3, and P4, semen and blood samples were collected 30, 60, 90, and 120 minutes, respectively, after intraperitoneal injection of 2.5 mg PGF2α/kg BW. Upon treatment completion, all the rats were euthanized with Zoletil at 40 mg/kg BW. Microscopic examination of spermatozoa quality, including motility, concentration, viability (survival), and abnormalities, was conduc...
Yugang Meng1*, Xiting Yan2 and Haiying Liang3
...he study group was given injection of goserellin on the basis of the control group. The changes of serum VEGF, EMAb, CA125 and sex hormones (FSH, LH, E2, P) were compared before and after treatment. Adverse reactions were recorded during the treatment The occurrence and recurrence were followed up for 1 year. After treatment, the total effective rate of the study group was 95.65%, which was significantly higher than the control group of 80.43% (P <0.05). Af...
Abdullah Channo1,2*, Asmatullah Kaka1, Akeel Ahmed Memon1, Mool Chand Malhi3, Muhammad Bakhsh4, Qudratullah Kalwar5, Shakeel Ahmed Tunio6 and Muhammad Ibrahim Panhwar7
...ny; Ghumanrabad with one injection prostaglandin (pgf2α) protocol and 13 animals showed estrus and were inseminated. Meanwhile, 8 were found pregnant through rectal palpation with a pregnancy rate of 61.53%....
Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Hasnaa Mallouk1, Mouloud Lamtai1, Zineb El Marzouki1, Amal Dimaoui1, Abdelhalem Mesfioui1, Aboubaker El Hessni1
...hate buffer saline (PBS) injection on postnatal day 2 (PND2). Brain regions were collected for biochemical analysis. The second cohort consisted of 20 rats, with equal gender distribution, divided into PBS- and LPS-injected groups. At PND60, these rats underwent behavioral tests (Open Field, Elevated Plus Maze, and Forced Swim), followed by biochemical analysis of brain structures. The findings showed a prolonged elevation of NO following the early immune chal...
Idrissi Ouedrhiri Housna1, Bikri Samir1,2*, Benloughmari Douae1, Aboussaleh Youssef1
...a single intraperitoneal injection of streptozotocin (STZ). Stress symptoms were induced using the chronic immobilization stress paradigm. Anxiety and depression-related behaviors were evaluated using three behavioral tests after treatment periods of 30 days (T1) and 60 days (T2), respectively. Blood samples collected post-sacrifice were analyzed to assess lipid parameters and corticosterone (CORT) levels. Additionally, organs (pancreas and brain) were dissect...
Rawya Sh. Mohammed*, Baraa N. Al-Okaily
...received intraperitoneal injections of doxorubicin (4.40 mg/kg B.W), Group G2 was administered CurSeNPs (10.47 μg/kg B.W), and Group G3 received both doxorubicin and CurSeNPs at the same doses. Blood samples were collected from anesthetized rats; serum was analyzed to assess levels of FSH, estrogen, TGF-β, and NF-κB. Additionally, ovarian tissue was extracted to measure levels of malondialdehyde (MDA) and superoxide dismutase (SOD) concentrations...
Emad M. Gad1, Haidy G. Abdel-Rahman2, Mohy Eldin Abd-El-Fattah1, Merna M. Kamal1, Ahmed Shaker Eltahan3, Amina A. Dessouki3*
...eived an intraperitoneal injection of a low single dose of STZ (40 mg/kg) after being fasted for the whole night. Sixty albino rats were separated into six equivalent groups. Group I control rats administrated 0.5ml distilled water by gavage for six weeks, group II untreated diabetic rats, group III–VI are diabetic groups; received dapagliflozin (1 mg/Kg daily) by gavage for 6 weeks, mulberry fruit extract (300mg/kg b.wt) by gavage, mulberry leaves extra...
Bingzhao Yan1, Guiping Chen2, Lulu Ding1, Ruxue Huang1, Wenjing Yu1 and Jicang Wang1*
...by daily intraperitoneal injection of CdCl2 solution and/or daily Que solution by gavage, respectively. Body weight, testis organ coefficient, and morphological changes of testis were measured. The related genes and protein expression levels of the PERK-CHOP pathway were also detected. The results showed that body weight and testicular organ coefficient decreased in cadmium-exposed rats compared with the control group, and pathological tissue sections showed a...
Adel Sobhy1, Ahmed El-khamary1, Ahmed M. Rashwan2,3, Ashraf Shamaa4, Mostafa Kassem5, Mohamed M. A. Abumandour6*, Ahmed El-Mansi7, 8 and Ahmed G. Nomir2
...involved brachial plexus injections at both sides of p1 and p2 (standing position). Our results from the cadaveric US showed that the brachial plexus root was sufficiently stained at site p1 as opposed to site p2. In the live experimental study, the brachial plexus appeared as a hyperechoic cluster at the p1 site, while the large and small rounded regions at the p2 site were hypoechoic. The distance at site P1 was 4.8±0.2 cm using 15 ml of Lidocaine loc...
Manar Mousa Alhussein*, Eman Faisal Albghdady
...ts received subcutaneous injections of 50 µg/rat of estradiol benzoate dissolved in 0.25 ml of olive oil for 15 successive days. After 24hrs from the last treatment session, rats in both groups were weighed, put under general anesthesia, and blood samples were taken to estimate testosterone and estrogen serum concentrations using competitive ELISA kits. The scrotum was dissected, the epididymis detached from the testis, and the weight and length were tak...
Nguyen Phi Bang1,2*, Nguyen Thi Hanh Chi1,2, Ngo Thuy Bao Tran1,2, Nguyen Thi Bich Hanh1,2, Nguyen Ba Trung1,2, Le Thi Thuy Hang1,2
...deg;C, 1 ml subcutaneous injection each dosage). Storage conditions and vaccine administration are carefully monitored to maintain consistent vaccine quality. However, factors related to dogs’ health in this area have significantly affected the vaccination’s effectiveness. This study was conducted to determine the factors affecting the efficacy of the Rabisin vaccine in preventing rabies in dogs in An Giang province (Vietnam). The study surveyed 34...
Kai Wang, Weifeng Xu, Pengyuan Dai and Chunmei Li*
... or 13 g/kg Arg) and PNP injection (0 or 100 mg/kg b.w.). Dietary Arg and subcutaneous injection PNP were performed simultaneously. Rats were sacrificed on d 8. Treatment of rats with 13 μg/ kg Arg resulted in a significant increase in mean daily weight gain (ADG), liver weight, and liver coefficient. However, Arg treatment in the PNP group showed an elevated trend relative to the rats treated with PNP alone, and the resu...
Pakistan Journal of Zoology
October
Featuring
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Molecular Detection and Genetic Characterization of Peste des Petits Ruminant Virus in Punjab, Pakistan
Javeria, Mudasser Habib and Muhammad Salahuddin Shah
Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2997-3000
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TGF-β Mediates the Role of P53 and microRNA-Related Signaling Pathways in the Pathogenesis of Diabetic Nephropathy in Rats
Yonghong Ma, Jinlan Ma, Qifu Zhang, Huajie Zou and Shenghua Ma
Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2993-2996
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Latrine Site Selection by the Asian Badger (Meles leucurus) in a Temperate Forest of South Korea
Ho-Kyoung Bae, Jae-Kang Lee, Tae-Kyung Eom, Dong-Ho Lee, Hyeongyu Ko, Joo-Hee Kim, Sung-Hyun Ahn and Shin-Jae Rhim
Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2989-2992
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Growth Performance of Labeo rohita Fingerlings Fed with Lactobacillus rhamnosus Supplemented Canola Meal and Corn Gluten-Based Feeds
Sadaf Aman, Yung Fu Chang, Javed Iqbal Qazi, Rahman Ullah and Farah Aman
Pakistan J. Zool., Vol. 56, Iss. 6, pp. 2985-2988
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