Antibacterial Effect of Co-Administration of Diclofenac and Ciprofloxacin against Infection Induced by Resistance E coli (O157-H7) in Female Rabbits
Antibacterial Effect of Co-Administration of Diclofenac and Ciprofloxacin against Infection Induced by Resistance E coli (O157-H7) in Female Rabbits
Frah Razzaq Kbyeh1, Ahmed N. Abedsalih2*
ABSTRACT
The aim of this project to study the effect of the combination between ciprofloxacin and diclofenac and alone in treating urinary tract infection caused by Escherichia coli (0157):H7. Samples were collected for the purpose of isolation and diagnosis resist.E. coli 0157:H7 two hundred urine samples were collected from adult women patients suffering from UTIs during a period from February 2022 to July 2022 in Diwaniyah city Iraq . All urine samples were incubated aerobically at 37°C for 24-48 hrs on blood agar, MacConkey agar as well as biochemical tests identified special media and the isolates. The isolates were confirmed and diagnosed by Vitek assay. In this study, forty-eighth adult female rabbit aged (6-8 weeks) and weighing between (1500 and 2000) gm., was randomly divided into six equal groups(8/each) as follow positive control(PC) , negative control(NC) diclofenac in 1mg/kg(DC), ciprofloxacin in 7 mg/kg(CIP), combination (ciprofloxacin 3.5 mg/kg+diclofenac1mg/kg) COM1 and combination(ciprofloxacin 1.75 mg/kg+diclofenac 1mg/kg) com2 groups all injection 0.1 ml in 1 x 109 CFU E coli 0157 :H7 by urinary catheterization route excepting in negative control and all groups excepting negative groups resulted significant increase(P< 0.05) in white blood cells, monocyte, and neutrophils in blood after three days comparison with negative control and in all experimental period (14 days) which was increase significant (P< 0.05) positive control (P.C.). However,(DC,COM1,COM2) groups decrease significant (P< 0.05) in white blood cells, monocyte, and neutrophils values after (seven, fourteen) days comparison with the PC and CIP groups. diclofenac can be used in combination with antibiotics as an anti-virulence agent which will enhance the ability of the immune system to eradicate infection. In histopathological examination clear damage in kidney tissue specimens from positive group control (PC,DC) observed, with hyperplasia endothelial cell swelling, ulcer in the lining membrane, compared with(CIP,COM1and COM2). Altogether, we can conclude that the diclofenac has the required anti-boiflim effects to support ciprofloxacin effect and the body responses against urinary tract infection consequences of E coli o157 :H7
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