Study The Pathological, Immunological Effects After Repeated Exposure to Tetrodotoxin in Liver of Albino Male Rats
Study The Pathological, Immunological Effects After Repeated Exposure to Tetrodotoxin in Liver of Albino Male Rats
Taghreed Jabbar Humadai*, Bushra Ibrahim Al-Kaisei
ABSTRACT
The current study aimed to examine the immunological and histopathological effects after intraperitoneal injection of tetrodotoxin (TTX) on liver of male albino rats. At the age of 8–9 weeks and weighing 190–200 gm, 60 male albino rats were separated into 3 groups: First group of 20 rats received distilled water and regular pellets. Second group of 20 rats received weekly injections of 0.5 µg/kg b.w. by I/P route, which was equivalent to 1/20 LD50 of tetrodotoxin. Third group of 20 rats received weekly injections of 1 µg/kg b.w. by I/P route, which was equivalent to 1/10 LD50 of tetrodotoxin. All animals were sacrificed at the conclusion of the experiment (90 days) in order to conduct biochemical, histopathological, and Immunohistochemical analyses. The oxidative stress result showed elevation in Malon Di-Aldehyde concentration in liver tissue treated with tetrodotoxin. The pathological lesion in liver of animals exposed to toxic dose of tetrodotoxin was characterized by dilation and congestion of portal vein, Focal aggregation of mononuclear cells formation granulomatous like lesion, Various form of nuclear necrosis with number apoptotic hepatocyte, nuclear pyknosis with evidence of councilman body in liver tissue, The Immunohistochemistry study revealed an increase in the scoring of IL1 expression in liver that showed score (3) compared to control group, TNF alpha expression showed score (1) in liver tissue when compared to untreated control group . According to the findings of the current study, tetrodotoxin has adverse toxic effects on liver tissue of male albino rats.
Keywords | Tetrodotoxin, Pathological changes, Oxidative stress, IL1, TNF alpha, Rats
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May 2024
Vol. 12, Iss. 5, pp. 802-993
