Asma-ul-Husna1, Muhammad Sajjad Ansari2, Bushra Allah Rakha3, Rabea Ejaz1, Nemat Ullah1 and Shamim Akhter1*
Asmatullah Kaka2, Wahid Haron1,*, Rosnina Yusoff 1, Nurhusien Yimer1, A.M Khumran1, Akeel Ahmed Memon2, Kazhal Sarsaifi1 andMahdi Ebrahimi3
... 24 hours. Successively, thawing and evaluation was performed for sperm motility, viability, membrane integrity, morphology, malondialdehyde (MDA), fatty acid composition and super oxide dismutase (SOD). Sperm characteristics such as sperm motility, viability, morphology, membrane integrity, and acrosome integrity were decreased in treated groups. DHA concentration was increased in all supplemented groups. Whereas ALA decreased in all groups except at 5ng/ml D...
Zakirullah1, Hamayun Khan1, Ali Gohar2, Haq Amanullah1,*, Muhammad Younas1, Muhammad Tarique Tunio3, Muhammad Saleem Khan4, Ibrar Muhammad Khan1 and Noushad Khan5
....
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Amit Sharma1* and Pankaj Sood2
...ation time, freezing and thawing rate. All these factors should be given due credit for achieving better post-thaw post thaw quality of semen to be utilized in different advanced reproductive techniques. ...
Noor Bad Shah Khattak1, Hamayun Khan1, Muhammad Tarique Tunio2, Shakoor Ahmad1, Nazir Ahmad3, Syed Murtaza Hassan Andrabi4, Obaidullah5, Ihsan Ali1,5,* and Nan-Zhu Fang5,*
...a following freezing and thawing. It is suggested that the addition of 1.5% green tea extract (4 g in 200 ml methanol) can be safely used for enhanced effectiveness of fertility after thawing.
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Muhammad Tariq1, Imtiaz Rabbani2, Muhammad Shahbaz Yousaf2, Imdad Ullah Khan1*, Hafsa Zaneb2, Sajid Iqbal3, Alam Zeb Khan1, Shakirullah1, Muhammad Shuaib Khan1, Atta Ur Rehman1 and Mumtaz Ali Khan2
...ilibration, freezing and thawing stages. They damage functional and morphological characteristics of sperms. Beside these exogenous stresses there is an oxidative stress which damages the spermatozoa endogenously. These stresses can be controlled with the inclusion of antioxidants in semen extender at the time of cryopreservation. In the current study, semen from four (n=4) healthy Nili Ravi buffalo bulls was collected through artificial vagina and Gallic acid...
Muhammad Hammad Fayyaz*, S. Murtaza Hassan Andrabi and Muhammad Shafiq Haider
...YS and Control). At post-thawing, progressive motility, plasma membrane integrity (PMI), and livability of buck spermatozoa were higher (P< 0.05) with 1mM GLU and 10mM CYS as compared to control. In the second experiment, post-thaw progressive motility, PMI, and livability were higher (P< 0.05) with 1mM GLU + 10mM CYS and 1mM GLU + 5mM CYS compared to other doses of GLU+CYS and control. We concluded that optimized supplementation of Glutathione and L-cys...
Ezz Ibrahim Khalifa, Ahmed Loly Ibrahim Desoky, Adel AbdElaziz Elbadawy, Gamal Ibrahim El-Emam, Tarek Messlam Mahmoud Mahdy*
... Also, E3 indicated post-thawing sperm parameters as motility, live, normal, and integrity acrosome up to 47.50, 65.10, 65.66, and 69.67%, but it was 37.22, 49.78, 55.89, and 59.22% relative to E0 extender, respectively. Furthermore, ATP, BC, and LPO levels post-thawing were 0.35µM, 255.40 CFU/ml, and 2.61 µM with E3 extender however, 0.19 µM, 276.00 CFU/ml, and 2.89 µM with E0 extender, respectively....
Nurul Isnaini1*, Ervin Kusuma Dewi Reksadinata1, Faizal Andri1, Tri Harsi2, Ida Zahidah Irfan2
...y, before freezing, post-thawing, and during recovery across age (p > 0.05). Semen volume, total sperm, total motile sperm, and frozen semen production increased in bulls from 6 to 7 years of age and then gradually reduced from 7 to 10 years of age. It could be concluded that the optimum semen productivity of Aceh cattle is when they are 7 years old.
Keywords | Artificial insemination, Frozen semen production, Indonesian local cattle, Reproductive p...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
...05) increased, while the thawing and cooking loss of meat were decreased (p < 0.05) in response to the increase in the dietary MWM levels, up to 6%. Furthermore, some physiological aspects of the broilers, such as total protein, albumin, globulin, triiodothyronine hormone, triglycerides, and cholesterol profile concentrations in the serum were significantly (p < 0.05) improved by increasing the level of MWM into broiler diets up to 6%. In contrast, some ...
Athhar Manabi Diansyah1, Muhammad Yusuf2*, Abdul Latief Toleng2, Muhammad Ihsan Andi Dagong2, Tulus Maulana3, Hasrin4, Abdullah Baharun5
... to identify sperms post-thawing quality and proteomic plasma semen as fertility performance in Bali-polled bull. The utilization of Bali-horned bulls as a point of reference was necessary to achieve this aim. The semen samples from Bali-horned and Bali-polled bulls were obtained twice weekly using an artificial vagina. The semen samples were immediately sent to the laboratory for processing. Parameters measured were sperm motility, abnormality, viability, acr...
Tarique Ahmed Khokhar1*, Atta Hussain Shah1, Muneer Ahmed Jamali1 and Asghar Ali Kamboh2
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...24 hours before the post-thawing examination. Results showed that spermatozoa diluted with egg yolk citrate supplemented with 3% lycopene (P3) had better quality than that found in the other four treatments, namely motility before freezing (70.86%) and post thawing (53.00%); viability before freezing (76.00%) and post thawing (60. 14%); plasma membrane integrity before freezing (71.29%) an...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of variance patterns in One-Way ANOVA. 0 µM, 10 µM, 30 µM and 50 µM genistein treatment in the basic diluent tris aminomethane–egg yolk. Progressive motility post-thawing at 1 hour and 2 hours was highest at genistein concentration 30 µM (P<0.05). The effect...
Abdel-Khalek El-Sayed Abdel-Khalek1*, Abdelaziz M. Sakr2, Wael M. Nagy2, Hesham S. Abou-Serie2, Entesar Z. Eliraqy2
...ilibrated semen and post-thawing in semen thawed in a water bath at 37oC for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total antioxidant capacity and superoxide dismutase activity, and decreased percentage...
Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3
... four weeks, followed by thawing at 4°C for 24 hours. The assessment covers thaw, cooking, and drip losses, along with shear force measurements. Muscle fibers in the breast and thigh were studied via Scanning Electron Microscopy. Using a Completely Randomized Design, meat quality records underwent analysis with four treatments: 10 weeks age of CBN (10CBN), 12 weeks age of CBN (12CBN), 10 weeks age of YWN (10YWT), and 12 weeks age of YWN (12YWT). Findings h...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...lity of spermatozoa post-thawing. This study aimed to assess the impact of incorporating lycopene into both the skim milk and egg yolk extender at varying doses on pre-freeze and post-thawed Kalang buffalo semen. Four Kalang buffalo, aged between 6 to 7 years and weighing 500-550 kg, were involved in this investigation. Semen collection was conducted weekly a 12- week period using an artificial vagina. Lycopene was added to the skim milk-egg yolk diluent at co...
Kenyum Lollen1, Judith Betsy C1*, Cheryl Antony2 and Stephen Sampath Kumar J3
...lity sperm. Freezing and thawing rate are the most critical factor in the cryopreservation of milt. In the present study, variability between spermatological parameters of induced and non-induced C. carpio was documented for a period of 7 months. Further, the non-induced milt was stored for 24 h at three dilution ratio (1:10, 1:20 and 1:40) and spermatological parameters were analysed. Then the milt was cryopreserved with the best dilution ratio using 3 freezi...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...tion time and post thawing cryopreservation in gaga chicken semen. The research material was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopical...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...ling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District Animal Health Center from August to September 2023. Semen diluted to a minimum of 25 million/mL is packaged in 0.25 mL str...
Makruf Arif1, Asmarani Kusumawati2*, Kurniawan Dwi Prihantoko3, Agustina Dwi Wijayanti4, Sri Gustari2
...tion, equilibration, and thawing. The evaluation criteria included sperm motility, recovery rate, viability, membrane integrity, and DNA fragmentation. In the post-dilution and post-equilibration phases, there was no significant effect on motility, viability, and membrane integrity (P > 0.05). However, supplementing genistein and glutathione improved the post-thawing results significantly (P < 0.05). The addition of 10...
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
...asma were analyzed after thawing (%). C5 and E15 extenders resulted in higher total motility (p 0.05) than the other extenders, with the exception of C10 and C15, which were lower than that of the control. The extender E15 (P <0.05) exhibited the highest motility. Furthermore, compared with the other extenders, the C5 and E15 extenders led to better vitality and membrane plasma in post-thawed spermatozoa (p 0.05). Finally, compared with the other extenders,...
Sumaya Loay Mohamed Shams Al-Dean1*, Sura Shakir Hammoud2, Mohammedali J. Ghafil3
...cally significant. After thawing, the sperm cells’ motility dramatically increased with the addition of 2 mM (56.8±1.16), 4 mM (57.6±1.36), and 7 mM (62.1±1.67). The sperms viability is improved post-thawimg and at 5C after addition of anti-oxidants vitamin C. When compared to the control values, the fresh, 5C, and post-thaw sperm motility improved significantly for each concentration of vitamin E. The p-value for 2mM(0.030), 4mM(0.0...
Siew Ing Nguang, Nur Syafiqah Binti Zainal, Ahmad Mukhlis Bin Hafas, Hou Chew Ha, Connie Fay Komilus, and Asmad Kari*
...tial during freezing and thawing. Each fish species requires a specific extender because of diverse sperm characteristics. Tilapia (Oreochromis sp.) popular in Malaysia for its hardiness and rapid growth is an excellent choice for aquaculture. Aloe vera (Aloe barbadensis) (Av) shows promise in spermatogenesis, but its use as a cryoprotectant in semen extenders is still underexplored. This study investigates the effects of antibiotic- and sugar-fr...
