The Effects of Various High Dosage of α- Tocopherol and Ascorbic Acid in Tris Egg Yolk Extender on Post-Thawed Sperm Quality in Tropical Brahman Bulls
The Effects of Various High Dosage of α- Tocopherol and Ascorbic Acid in Tris Egg Yolk Extender on Post-Thawed Sperm Quality in Tropical Brahman Bulls
Langgeng Priyanto1,2*, Herdis2, Santoso2, Pradita Iustitia Sitaresmi2,
Tri Puji Priyatno2, Rahma Isartina Anwar2, Desiana Ade Mahari2,
Florentina Bety Indah Lupitasari2, Maman Surachman2,
I Wayan Angga Darmawan2, Arfan Abrar1 and Arum Setiawan3
ABSTRACT
The primary causes of sperm quality decline during the freeze-thaw pathway are the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Alpha-tocopherol (Vitamin E) and ascorbic acid (Vitamin C) are two potent antioxidants that operate to prevent oxidation processes. The objective of this study was to analyze the effects of α- tocopherol and ascorbic acid on the motility, viability, abnormality, and plasma membrane quality of post-thawed Tropical Brahman bull sperm. Sperm samples were obtained and pooled from eight sexually mature Tropical Brahman Bull, separated into seven equal proportions, and diluted with TEY extender Semen was supplemented with 0 (C0;E0), 5 (C5;E5), 10 (C10;E10), and 15 (C15;E15) mg/mL of each α-tocopherol and ascorbic acid, respectively. Total sperm motility, viability, abnormalities, and semen membrane plasma were analyzed after thawing (%). C5 and E15 extenders resulted in higher total motility (p 0.05) than the other extenders, with the exception of C10 and C15, which were lower than that of the control. The extender E15 (P <0.05) exhibited the highest motility. Furthermore, compared with the other extenders, the C5 and E15 extenders led to better vitality and membrane plasma in post-thawed spermatozoa (p 0.05). Finally, compared with the other extenders, abnormalities were lower in the C5-and E10 extenders (p<0.05). Finally, the current findings show that C5 and E15 can increase the quality of post-thawed Brahman bull spermatozoa.
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