Xiangxing Zhu1, Junyu Nie1, Shouneng Quan1,2, Huiyan Xu1, Xiaogan Yang1, Yangqing Lu1, Kehuan Lu1 and Shengsheng Lu1*
...though a large number of transgenic mouse models carrying astrocyte-specific transgene expression of transgene have made significant contributions to understanding astrocytic structure and function in vitro and in vivo, evidence suggests that mouse models are limited and sometimes do not fully replicate the complete spectrum of neurological phenotypes seen in human diseases. On the contrary, pigs, especially mini-pigs, show exciting potential for modeling huma...
Talha Nazir1,2,*, Muhammad Dildar Gogi2, Muhammad Zeeshan Majeed1, Waheed ul Hassan2, Abdul Hanan2 and Muhammad Jalal Arif2
... has been a challenge on transgenic (Bt) cotton crop all over the world. This study assessed the field efficacy of six commercial insecticides viz.; Blaster 72.5%WP (imidacloprid + acephate), Bugatti 50%SC (imidacloprid + bifenthrin), Confidor 20%SL (imidacloprid), Jozer 202SL (imidacloprid + acetamiprid), Pouch 35%SC (pyriproxyfen + etofenprox) and Senator 41.6%EC (imidacloprid + pyriproxyfen) against sucking insect pest complex and also determined the...
Zaheer Abbas*, Shaukat Ali, Jalal-Ud-Din and Ghulam M. Ali*
...fficiency to produce new transgenic maize lines expressing desirable agronomic characteristics. ...
Imran Ali Rajput1*, Tajwer Sultana Syed1, Ghulam Hussain Abro1, Imran Khatri1 and Abdul Mubeen Lodhi2
...nce. The introduction of transgenic cotton since last decades has played a significant role in suppressing the pest population of Pectinophora gossypiella (Saund) which is the main pest of cotton in all over the world. Nevertheless, the indigenous botanical extracts have been used effectively against various sucking pests of cotton but only few studies have been attempted to observe their action against bollworms. Therefore, the present study has bee...
Sabahat Noor*1, Shaukat Ali1, Hafeez-ur-Rahman1, Farhatullah2 and Ghulam Muhammad Ali1
...on. In this study the T2 transgenic (At-DREB1A) and non-transgenic line of lasani-08 from single transformation event was exposed to 15 days drought and different levels of salt concentrations separately. Four parameters i.e. sugar contents, proline contents, chlorophyll contents and relative water contents were recorded and compared in transformed and non-transformed plants. It was observed that under drought and salt stres...
Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*
...evelopment, we generated transgenic porcine embryos by handmade cloning. We then assessed the embryonic developmental rate at cleavage and blastocyst stages. Our results showed that the HSPC117 transgenic embryos had markedly higher cleavage and blastocyst rates when compared to the embryos with pcDNA 3.1 vector, (69.7 ± 3.5% vs. 64.6 ± 1.8%, and 24.8 ± 2.2% vs. 15.9 ± 4.3%, r...
Muhammad Irfan Ullah1*, Muhammad Arshad1, Muhammad Imran Khan1, Muhammad Afzal1, Azhar Abbas Khan2, Syed Muhammad Ali Zahid1, Muhammad Saqib1, Asad Abdullah1, Saba Kousar1 and Maryam Riaz1
...and CIM-599) and one non-transgenic genotype (CIM-554) for the performance of H. armigera feeding The difference in leaf injury, relative consumption and growth rate of H. armigera was detected among experimental factors and their interactions. The leaf injury caused by H. armigera was higher (38.5 cm2 and 30.5cm2) on non-transgenic genotype at high and low moisture levels of plants respectively. Similarly, the relative grow...
Hala Rajab1, Muhammad Sayyar Khan1*, Safdar Hussain Shah1 and Syed Mehar Ali Shah2
... Successful selection of transgenic lines was achieved using selective antibiotic kanamycin 50 mgL-1. NtSAT4 gene integration into the plants genome was confirmed by PCR using the NtSAT4 gene specific primers. The positive transgenic lines were successfully acclimatized to soil and glass house conditions. The developed transgenic lines have the potential to overproduce high levels of cyste...
Betül Ayça Dönmez, Sarbesh Das Dangol and Allah Bakhsh*
...justify;">Development of transgenic potatoes is imperative to investigate various gene functions as well as to develop robust potato varieties resistant to different biotic and abiotic stresses, specially in diploid potato and to redirect the breeding program away from tetraploids to the diploids. We aimed to develop the protocol for transformation of Solanum chacoense diploid M6 potato using tetraploid S. tuberorsum cv. Desiree as a control using five differe...
Hafiz Muhammad Tahir*, Palwasha Jabeen, Chand Raza, Shaukat Ali
...ques including of making transgenic organisms.
...
Anila Latif, Zaheer Abbas, Farhatullah and Ghulam Muhammad Ali*
...ation of the BBE gene in transgenic lines was confirmed through PCR while the expression was confirmed by Reverse Transcriptase PCR. Whole plant bioassay confirmed 100% mortality in DBM within 72 hours. The present investigation concludes that the metabolic engineering of berberine in plants is an effective strategy to protect plants from herbivorous insects and this pathway should further be evaluated for possible application in agriculture. ...
Muhammad Medrar Hussain*, Asad Jan* and Sayyar Khan Kazi
...i. From these rice calli transgenic plants were regenerated and established in greenhouse. For the evaluation of OsTZF8 gene role in drought stress tolerance, two weeks old rice seedling were subjected to drought stress. OsTZF8-OX transgenic indica line A and B displayed 69% and 64% survival rates respectively compared to 33% of control. These results confirmed that due to overexpression of OsTZF8 gene,
Muhammad Jan1*, Safdar Hussain2, Muhammad Anwar ul Haq3, Javed Iqbal4, Ilyas Ahmad4, Muhammad Aslam4 and Aiman Faiz3
MA Liman1, QI Yongxiao1, Wang Wenji2* and Zhong Qianyi3*
...fli1-EGFP) transgenic zebrafish with fluorescent blood vessels were studied to evaluate vascular development defects. G-Rh2 treatment up to 84.85 μM did not induce morphological defects in zebrafish. We observed suppression effect of angiogenesis by G-Rh2 in inhibiting intersegmental vessel formation from 30 to 84.85 μM in a dose-dependent manner. Moreover, quantitative real-time PCR revealed that G-Rh2 suppressed vascular endothelial growt...
Aya El-Turkey1, A. K. El-Attar1, A. E. Aboulata1, B. Othman2 and K. A. El-dougdoug2
...nd protein levels in the transgenic tomato plants. Finally, we report here, the use of stable transformation to develop transgenic tomatoes expressing the biologically active HSV-2 gD antigen (gD2). Thus, our results may have implications for the development of plant based vaccine production systems against HSV-2....
Kai Jin1,2,3, Chen Chen 1,2,3, Xinyu Sun1,2,3, Caiye Zhu1,2,3, Mahmoud F. Ahmed1,2,3,4, Qisheng Zuo1,2,3, Jiuzhou Song4 and Bichun Li1,2,3*
...bility and expression in transgenic SCD1 mice and goats. Our results suggested the possibility of generating transgenic goats by sperm-mediated gene transfer (SMGT). The expression of SCD1 gene and fatty acid metabolism genes in goat mammary gland tissues and muscular tissues was aligned with GEO database, and tested by qRT-PCR. F1 transgenic mice were mated and then the ...
Tamoor Ali1*, Muhammad Hamid Bashir1, Bilal Saeed Khan1, Abdul Ghaffar2, Danish Riaz3, Muhammad Zia ul Haq1, Shoaib Nawaz1, Aneeb Ali1, Iftikhar ul Hassan1, Ikram Ul Haq4, Muhammad Khuram Shahzad1, Usama Saleem5 and Tamsila Nazir2
...t pest fauna against the transgenic and non-transgenic cotton varieties. To figure out the response of these pest with the meteorological factors against whitefly, thrips and jassid. For this experiment RCBD Design was used. The five varieties of cotton were used in which IUB-2015, MNH-886, FH-142 and CEMB-33 were the Bt varieties while FH 942 was the non-Bt. The highest population of whitefly (4.707/leaf), thrips (4.073/lea...
Mustafa Ozan Atasoy
...istic, relatively higher transgenic capacity, and enabling the DIVA strategy. Numerous bivalent and multivalent HVT constructs have been generated by various methods and some of these constructs have been made commercially available so far. The efficacy of HVT-vectored vaccines has been assessed either individually or in combination with other vaccine formulations; hence, the optimal competence of these vaccines against viral diseases has been reported. Based ...
Muhammad Shafiq1, Tehseen Ashraf2*, Sehrish Mushtaq1, Naveeda Anjum3, Muhammad Asim4, Muhammad Aqeel Feroze3, Malik Abdul Rehman4 and Marja Aziz3
...vealed that the putative transgenic calli were not transformed and died after 40-60 days. The experiment was repeated ten times to see the success rate of the regeneration system. In this study a protocol for chili plant regeneration was developed for callus induction, plant regeneration, and plant transformation. Because developing a viable chilli tissue culture and plant regeneration system is difficult, this study was designed to help develop one. SP was fo...
Kalsoom1, Nasir Shah2, Muhammad Ibrahim3*, Tahira Bibi1, Kazim Ali4 and Zahir Shah2
...ild (Sante wild) and two transgenic (Sante 2 event and Sante 8 event) potato varieties in in vitro condition. These varieties were grown on Murashige and Skoog (MS) media subjected to (0, 25, 50, 75 and 100 mM) of Sodium Chloride (NaCl). Potato varieties showed an adverse in vitro growth response to all levels of salt in MS media, while plants of all the tested varieties were dead at concentrations more than 50 mM of NaCl in in vitro condition (75, 100 mM). Va...
Aqleem Abbas1, Mustansar Mubeen2, Yasir Iftikhar2, Qaiser Shakeel3*, Hafiz M. Imran Arshad4, Maria del Carmen Zuñiga Romano5 and Sarfaraz Hussain6
...s including mycoviruses, transgenic approaches, gene loci/markers, gene silencing, and genome editing are also summarized. Finally, the expression of resistant-related genes in rice crops and biological agent mechanisms of action are discussed....
Fariha Qahar and Muhammad Sayyar Khan*
...ids, and mitochondria in transgenic lines, resulted in enhanced tolerance in terms of lesser wilting and pigment discoloration to induced stress compared to non-transformed plants. In terms of approximate percentage damage, under 14% H2O2stress,30-60% of the leaf area turned necrotic in the single overexpression lines compared to 95% damage in the wild-type plants. Whereas, the least amount of damage (10-20%) was observed in the double overexpression lines. Wh...
Shruti Yadav1,2*, Kamana Singh2, Pratap Adinath Divekar3 and Suhas Gorakh Karkute1,3*
...lants of six independent transgenic events. Cry2Aa gene was highly expressed in transgenic plants and its protein level was as high as 30.94 µg/g in fresh leaves and 20.57µg/g in fruits. Insect bioassay showed the BSFB larval mortality between 90% to 100%. Altogether it was observed that expression of the Cry2Aa protein in the shoots and fruit of transgenic brinjal lead to high...
Wali Muhammad Mangrio1*, Hakim Ali Sahito1, Abdul Hafeez Mastoi2, Sanaullah Sattar3, Fahmeeda Imdad Sahito4 and Shahid Ali Jakhrani1
...nsect pests on promising transgenic and non-transgenic cotton genotypes in farmer’s field condition, Naushahro Feroze. The seeds of three popularly grown genetically hybrid cotton varieties namely; KMG-1, KMG-2, KMG-3, and non-Bt NIAB-78 were sown on 8th May 2023. After 6th week of cotton sowing, the data of pest infestation were gathered through randomized complete block design on a weekly interval basis on recommende...
