Evaluating the Performance of Genetically Engineered Serine acetyltransferase 4 (NtSAT4) Overexpression Brassica napus L. Lines under Xenobiotics Exposure
Evaluating the Performance of Genetically Engineered Serine acetyltransferase 4 (NtSAT4) Overexpression Brassica napus L. Lines under Xenobiotics Exposure
Fariha Qahar and Muhammad Sayyar Khan*
ABSTRACT
Glutathione (GSH) is a powerful antioxidant thiol compound that is critical for the detoxification of xenobiotics in plants. The genetic manipulation of GSH biosynthesis-related genes is considered a prime strategy to achieve higher in planta GSH contents. In this study, stably transformed Brassica napus lines harboring the feedback-insensitive isoform of Serine acetyltransferase (SAT), a rate-limiting enzyme for cysteine (Cys), and GSH biosynthesis, were subjected to H2O2, metolachlor, and atrazine-induced oxidative stress. The overexpression of the NtSAT4 gene from Nicotiana tobacco under 35S promoters in various compartments of the cell, which includes cytosol, plastids, and mitochondria in transgenic lines, resulted in enhanced tolerance in terms of lesser wilting and pigment discoloration to induced stress compared to non-transformed plants. In terms of approximate percentage damage, under 14% H2O2stress,30-60% of the leaf area turned necrotic in the single overexpression lines compared to 95% damage in the wild-type plants. Whereas, the least amount of damage (10-20%) was observed in the double overexpression lines. When subjected to 24 µM metolachlor, the wild-type leaf discs were fully necrotic, whereas the single overexpression lines exhibited 20-60%, and the double overexpression lines showed only 15-20% necrosis. The data suggested that overexpression of NtSAT4 is a promising strategy for improved stress tolerance against these xenobiotics in B. napus.
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