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Analysis of Proximate Body Composition under the Influence of Different Ammonia Concentration in Confined Condition of Pangasius pangasius

Analysis of Proximate Body Composition under the Influence of Different Ammonia Concentration in Confined Condition of Pangasius pangasius

Shoaib Hassan and Muhammad Naeem*

Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan 60800, Pakistan.

 
*Correspondence | Muhammad Naeem, Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan 60800, Pakistan; Email: [email protected] 

ABSTRACT

Proximate body composition was analyzed to determine the effect of ammonia exposure on various body constituents (water, ash, fat, protein, and organic content) of P. pangasius. Oven dried method was used for estimation of water content; muffle furnace for ashing, chloroform: methanol method for fat content and protein content was estimated by the difference in mass of other constituents. Variation in the percentage of water and protein content was observed, and minimum water content and maximum protein content were observed in control (To). Regression analysis was done by a relationship of various body constituents with body size (weight and length), all of the constituents showed non-significant except relation with protein content as the control group showed a highly significant correlation (P<0.001; r=0.897) with percent water indicating inverse relation. While, condition factor showed non-significant correlation (P>0.05) with other body constituents except % ash with condition factor in T4. It is, therefore, concluded that body composition of P. pangasius can be estimated directly from water content, body weight or length of fish using predictive regression models developed in this work with a reasonable amount of accuracy.

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Pakistan Journal of Zoology

November

Pakistan J. Zool., Vol. 56

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