Evaluating the Performance of Genetically Engineered Serine acetyltransferase 4 (NtSAT4) Overexpression Brassica napus L. Lines under Xenobiotics Exposure
Evaluating the Performance of Genetically Engineered Serine acetyltransferase 4 (NtSAT4) Overexpression Brassica napus L. Lines under Xenobiotics Exposure
Fariha Qahar and Muhammad Sayyar Khan*
Effect of 14% H₂O₂ on the leaf discs of NtSAT4 single and double overexpression lines. Leaf discs from three compartment-specific single overexpressor lines cytosolic (C), mitochondrial (M), and plastidic (P) and double overexpressor lines mitochondrial×plastidic (M×P) and mitochondrial×cytosolic (M×C) lines and wild-type plants treated with 14% H₂O₂ for 24 hours. Scale bar = 1 cm.
Effect of metolachlor on the leaf discs of NtSAT4 single and double overexpression lines. Leaf discs of single overexpressor lines cytosolic (C), mitochondrial (M), and plastidic (P) and double overexpressor lines mitochondrial × plastidic (M×P) and mitochondrial × cytosolic (M×C) lines and wild-type plants exposed to 24µl metolachlor. Leaf discs were kept under continuous light for 24 hours at room temperature. Scale bar = 1 cm.
Effect of the herbicide atrazine on NtSAT4 single and double overexpressor lines and wild-type. One-month-old non-transformed (Wt), NtSAT4 single overexpressor lines mitochondrial (M) and plastidic (P) and double overexpressor lines mitochondrial × cytosolic (M×C) and mitochondrial × plastidic (M×P) plantlets grown on 1/4-strength MS hydroponic media. Plants were treated with control (1/4-strength MS), 12 µl atrazine for 48 hrs, and 24 µl for 24 hours. These were kept under control conditions in the growth room under continuous light. Scale bar = 8cm.
