Addition of Tomato Juice as Additive in Diluent of Egg Yolk Citrate on the Quality of Pesisir Cattle Semen
Addition of Tomato Juice as Additive in Diluent of Egg Yolk Citrate on the Quality of Pesisir Cattle Semen
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
ABSTRACT
This study aims to determine the effect of adding tomato juice as an additive in egg yolk citrate diluent on the quality of Pesisir cattle semen stored at 5oC. The study was conducted on freshly collected Pesisir cattle semen diluted in egg yolk citrate with or without tomato juice viz., P0 (100% egg yolk citrate), P1 (90% egg yolk citrate + 10% tomato juice) and P2 (80% egg yolk citrate + 20% tomato juice). After diluting the semen samples were stored at 5oC for 4 hours. The observed variables included spermatozoa motility, spermatozoa survival, spermatozoa abnormalities and intact plasma membranes of spermatozoa. The obtained data was analyzed using the Analysis of Variance (ANOVA). The results showed that the addition of tomato juice extract into egg yolk citrate diluent exhibited a 20% increase in sperm motility percentage, 26.3% in sperm survival rate and 22.5% in intact plasma membrane percentage as compared to the control group (P < 0.01). However, the sperms abnormality data did not show any effect (P>0.05) of tomato juice addition. The addition of 20% tomato juice extract in egg yolk citrate diluent showed better results as compared to the 10% tomato juice addition. In conclusion, tomato juice addition in egg yolk citrate diluent is beneficial to improve the sperm quality of Pesisir cattle semen stored at 5oC.
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