Antagonistic Probioticity of Novel Bacterial Isolates from Pakistan against Fish Pathogen Pseudomonas fluorescens in Labeo rohita Fingerlings
Antagonistic Probioticity of Novel Bacterial Isolates from Pakistan against Fish Pathogen Pseudomonas fluorescens in Labeo rohita Fingerlings
Asma Chaudhary1, Qurat-ul-Ain Ahmad1, Afia Muhammad Akram1, Sadia Roshan2 and Javed Iqbal Qazi3*
Effect of inocula sizes on growth of the probiotic isolates Sphingomonas sp. AsCh-P3 (a) and Bacillus aerius AsCh-A7 (b) in fish feed extract (20% w/v) at room temperature for different incubation days.
Symptoms of Pseudomonas infection i-e swelling of belly (a), swelling of anus (b), reddening of belly (c) and damage of scales (d) following i-p administration of P. fluorescens (268×103 C.F.U.ml-1).
Appearance of surviving fishes (asymptomatic) of negative control (a), positive control challenged with P. fluorescens (b), treated by Sphingomonas sp. AsCh-P3 (c-e) and Bacillus aerius AsCh-A7 (f-h) representing for different experimental groups G1a, G1b, G1c, G2a, G2b, G2c, respectively.
Surviving fishes with varying degrees of caudal fin deformation by P. fluorescens in G2 group (a, b) treated with B. aerius AsCh-A7 and muscle degeneration by P. fluorescens in positive control C3 (c) and experimental groups G2b, G3b (d, e) treated with Sphingomonas sp. AsCh-P3 and B. aerius AsCh-A7, respectively.
