Bacterial Expression and Purification of Antigenic Recombinant Protein Encoded by Hepatitis Delta Virus Antigen of Pakistani Isolate
Bacterial Expression and Purification of Antigenic Recombinant Protein Encoded by Hepatitis Delta Virus Antigen of Pakistani Isolate
Iram Amin1,2*, Shazia Rafique1, Nadeem Ahmed1, Muhammad Shahid1, Samia Afzal1, Tahir Rehman Samiullah1, Mohsin Ahmed Khan1 and Muhammad Idrees1,3
Fig. 1.
PCR amplification of HDV gene fragment, Lane 1-9, HDV DNA positive of 405bp and Lane 10 showing negative control, Lane 11, DNA marker of 50bp.
Fig. 2.
Restriction digestion analysis of clone in expression vector: Lane A, DNA marker of size 100bp; Lane B, first band digested of peT28a vector of size 5369bp and second band of HDAg-An gene fragment of size 402bp. Lane C, undigested clone; Lane D, ladder 1Kb.
Fig. 3.
A, SDS-PAGE of purified protein showing expression of 14KDa protein fragment. B, Western Blot representing 14KDa protein fragment of HDAg-An purified protein.
December 2022
Vol. 54, Iss. 6, Pages 2501-3000
